Comprehensive sequence and expression profile analysis of the phosphate transporter gene family in soybean

Abstract

The family of phosphate transporters (PHTs) mediates the uptake and translocation of Pi inside the plants. However, little is known about transporters in soybean. Therefore, Searched the Genome Database for Soybean, 57 GmPHTs family members were identified in soybean, Phylogenetic analysis suggested that members of the PHTs gene family can be divided into six clades. Collinearity analysis revealed that most of the GmPHT genes shared syntenic relationships with PHTs members in Arabidopsis thaliana and that large segment duplication played a major driving force for GmPHTs evolution in addition to tandem duplication. Further analysis of the promoter revealed that light-responsive elements and abiotic stress-responsive elements were widely distributed within the promoter regions of GmPHT genes. Based on RNA-seq data, GmPHTs showed different expression patterns in roots and leaves of soybean treated with long-term low phosphorus and short-term low phosphorus, in addition, the expression levels of GmPHT genes can be regulated by drought stresses, it was implied that the induced expression of GmPHTs could promote phosphorus uptake and transport in soybean and thus adapt to low phosphorus and drought stress, which is the first step dissection of Pi transport system and probably refers to new roles of PHTs genes in soybean.

Involvement of OsS40-14 in ROS and plastid organization related regulatory networks of dark-induced leaf senescence in rice

“…CUT&Tag is an enzyme-tethering method based on in situ chromatin tag mentation and used mainly for efficient profiling of epigenetic modification states in cultured animal cells (Kaya Okur et al, 2019;. Recently, CUT&Tag protocol has also been applied in plant tissues or cells for epigenomic analysis or determining binding landscape of transcription factors (Ouyang et al, 2022;Wu et al, 2022;Zhang et al, 2023). Thus, we developed the CUT&Tag manner combined with protoplast-based transient expression system for constructing the OsS40-14-bound DNA sequencing library and identified three targeted genes by ChIP-qPCR as well as 2311 potential bound genes according to the obtained CUT&Tag-seq data (Figure 6, Supplementary Figure S4).…”

“…In addition, nutrient remobilization from senescing leaves to developing tissues is important, so that precious nutrients, such as phosphorus and carbohydrates, are not lost to the environment upon abscission of the fully-senescent leaf. Three phosphate transporter genes OsPT5, OsPT19 and OsPT20, are upregulated during dark stress condition (Ye et al, 2015;Wei et al, 2022) and increased expression of these genes in flag leaf contributes to remobilization of phosphorus from senescing leaves to developing grains (Jeong et al, 2017). Another phosphate translocator glucose 6-phosphate (GPT) which is a plastid translocator gene importing glucose-6-phosphate (Glc6P) into plastids for starch synthesis.…”

Effects of Soybean Phosphate Transporter Gene GmPHT2 on Pi Transport and Plant Growth under Limited Pi Supply Condition

“…Globally, many soils are deficient in Pi, and the concentration of Pi inside plant cells is typically 1000 times higher than outside [ 7 , 8 ]. To counteract the large concentration gradient at the root–soil interface, plants must have specialized transport systems that transport Pi from the soil to the cells [ 9 ]. The active phosphorus in the soil and the phosphorus used in various metabolic processes eventually require direct uptake and transport through the “phosphate transporter protein (PHT)”, a highly efficient plant root uptake and transport system that is responsible for the transport of inorganic phosphorus in plants under transcription factor regulation [ 10 ].…”

NopC/T/L Signal Crosstalk Gene GmPHT1-4

“…The PHR-PHT1 module is integral to Pi stability, and high expression levels of GmPHT1 in root nodules contribute to increased Pi accumulation and nodule growth, in addition to enhancing nitroxidase activity [34,35]. GmPHT1-4, a member of the PHT family with 14 homologous genes, is highly expressed in the roots, similar to AtPHT1 in Arabidopsis, playing a critical role in phosphate uptake under both low and high phosphate conditions [36].…”