Comprehensive Quality Evaluation of Polygonatum cyrtonema and Its Processed Product: Chemical Fingerprinting, Determination and Bioactivity

Abstract

Processing of Chinese herbal medicines (CHMs) is a traditional pharmaceutical technology in Chinese medicine. Traditionally, proper processing of CHMs is necessary to meet the specific clinical requirements of different syndromes. Processing with black bean juice is considered one of the most important techniques in traditional Chinese pharmaceutical technology. Despite the long-standing practice of processing Polygonatum cyrtonema Hua (PCH), there is little research on the changes in chemical constituents and bioactivity before and after processing. This study investigated the influence of black bean juice processing on the chemical composition and bioactivity of PCH. The results revealed significant changes in both composition and contents during processing. Saccharide and saponin content significantly increased after processing. Moreover, the processed samples exhibited considerably stronger DPPH and ABTS radical scavenging capacity, as well as FRAP-reducing capacity, compared to the raw samples. The IC50 values for DPPH were 1.0 ± 0.12 mg/mL and 0.65 ± 0.10 mg/mL for the raw and processed samples, respectively. For ABTS, the IC50 values were 0.65 ± 0.07 mg/mL and 0.25 ± 0.04 mg/mL, respectively. Additionally, the processed sample demonstrated significantly higher inhibitory activity against α-glucosidase and α-amylase (IC50 = 1.29 ± 0.12 mg/mL and 0.48 ± 0.04 mg/mL) compared to the raw sample (IC50 = 5.58 ± 0.22 mg/mL and 0.80 ± 0.09 mg/mL). These findings underscore the significance of black bean processing in enhancing the properties of PCH and lay the foundation for its further development as a functional food. The study elucidates the role of black bean processing in PCH and offers valuable insights for its application.

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

“…Our research group conducted a comprehensive evaluation of the quality of PCH medicinal materials in the early studies. The results indicate significant differences in the active ingredients and pharmacological effects of PCH medicinal materials from different growing areas, especially in the Sichuan and Guangxi provinces (Zhang et al, 2023).…”

Utilizing Integrated UHPLC-Q-Exactive Orbitrap-MS, Multivariate Analysis, and Bioactive Evaluation to Distinguish between Wild and Cultivated Niudali (Millettia speciosa Champ.)

“…According to the conditions, wild and cultivated MSCP samples were comprehen- Ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS) has the advantages of excellent sensitivity, wide analytical range, better separation, and reliable qualitative analysis without elaborate sample preparatory procedures, which has been extensively used in identification of herbal medicines [10,11]. It can also be widely applied to identify components from different base resources and parts of traditional Chinese medicine [12][13][14][15]. Moreover, it can also further improve the resolution of unknown compounds, especially for poor chromatographic separation.…”

Characterization of Phenolic Compounds of Arnica montana Conventional Extracts

“…Finally, peaks 8 and 9 correspond to 6-methoxykaempferol (m/z 315) [7] and hispidulin (m/z 299). Considering the good results achieved when identifying the phenolic compounds, a more in-depth study to validate the present HPLC-MS/MS method in terms of linearity, precision, repeatability, stability, and recovery has been planned [8]. Considering the good results achieved when identifying the phenolic compounds, a more in-depth study to validate the present HPLC-MS/MS method in terms of linearity, precision, repeatability, stability, and recovery has been planned [8].…”