Comprehensive Profiling of Lysine Acetylome in Baculovirus Infected Silkworm (<i>Bombyx mori</i>) Cells

Hu, Dongbing; Xue, Shifeng; Zhao, Chen; Wei, Ming; Yan, Huihui; Quan, Yanping; Yu, Wengong

doi:10.1002/pmic.201700133

Cited by 21 publications

(31 citation statements)

References 74 publications

(82 reference statements)

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“…The presence of PTMs on viral proteins adds another layer of complexity to the role of acetylation during infection. Although recent work in insect and fish species revealed viral acetylomes as critical contributors to infection 67 , 68 , acetylation of viral proteins during human pathogenic infections remains understudied. Most of our understanding of viral protein acetylation comes from work on individual proteins, such as influenza A NP, HIV Tat, and papillomavirus E2 6 , 8 , 10 .…”

Section: Discussionmentioning

confidence: 99%

Orchestration of protein acetylation as a toggle for cellular defense and virus replication

2018

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Emerging evidence highlights protein acetylation, a prevalent lysine posttranslational modification, as a regulatory mechanism and promising therapeutic target in human viral infections. However, how infections dynamically alter global cellular acetylation or whether viral proteins are acetylated remains virtually unexplored. Here, we establish acetylation as a highly-regulated molecular toggle of protein function integral to the herpesvirus human cytomegalovirus (HCMV) replication. We offer temporal resolution of cellular and viral acetylations. By interrogating dynamic protein acetylation with both protein abundance and subcellular localization, we discover finely tuned spatial acetylations across infection time. We determine that lamin acetylation at the nuclear periphery protects against virus production by inhibiting capsid nuclear egress. Further studies within infectious viral particles identify numerous acetylations, including on the viral transcriptional activator pUL26, which we show represses virus production. Altogether, this study provides specific insights into functions of cellular and viral protein acetylations and a valuable resource of dynamic acetylation events.

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Section: Discussionmentioning

confidence: 99%

Orchestration of protein acetylation as a toggle for cellular defense and virus replication

2018

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“…Using KEGG online service tools KAAS (KEGG Automatic Annotation Server) (https://www.kegg.jp/kegg/kegg2.html) to annotated protein's KEGG database description. Mapping of the annotation results on the KEGG pathway database was performed using KEGG online service tools KEGG mapper (Hu et al ., ).…”

Section: Methodsmentioning

confidence: 97%

“…The GO with a corrected value of p <0.05 was considered significantly. The encyclopaedia of Genes and Genomes (KEGG) database (Hu et al ., ) was used to identify enriched pathways by a two‐tailed Fisher's exact test to test the enrichment of the differentially expressed individual proteins against all identified proteins. Significant enrichment was determined at the value of p < 0.05.…”

Section: Methodsmentioning

confidence: 99%

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

Fang

Klosterman

Tian

et al. 2019

Environmental Microbiology

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Summary The fungus Verticillium dahliae causes vascular wilt disease on more than 200 plant species worldwide. This fungus can survive for years in soil as melanized microsclerotia. We found that VdCmr1, a transcription factor, is required for the melanin production and increased survival following UV irradiation in V. dahliae but not for microsclerotia production or virulence. Here, we provided evidence how VdCmr1 protects against high temperature (HT) and UV irradiation in V. dahliae. The results indicate that VdCmr1 mediates entry to the diapause period in V. dahliae in response to HT and contributes to the expression of proteins to minimize protein misfolding and denaturation. VdCmr1 deletion results in the misregulation of DNA repair machinery, suggestive of reduced DNA repair capacity following UV irradiation and in correlation with the low survival rate of UV‐treated VdCmr1 mutants. We discovered a putative VdCmr1‐dependent gene cluster associated with secondary metabolism and stress responses. We also functionally characterized two VdCmr1‐responsive genes participating in HT and UV response. These results shed further light on the roles of VdCmr1 in protection from HT or UV irradiation, and the additional insights into the mechanisms of this protection may be useful to exploit for more effective disease control.

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“…Significantly expressed genes with a |log 2 (fold‐change)| > 1 and P ≤ 0.05 were selected. The encyclopedia of Genes and Genomes (KEGG) database (Hu et al , 2018) was used to identify enriched pathways by a two‐tailed Fisher’s exact test to test the enrichment of the differentially expressed individual gene products against all those identified. Significant enrichment was determined at P < 0.05.…”

Section: Methodsmentioning

confidence: 99%

The bZIP transcription factor VdAtf1 regulates virulence by mediating nitrogen metabolism in Verticillium dahliae

Tang

Klosterman

et al. 2020

New Phytologist

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The fungus Verticillium dahliae causes vascular wilt disease on hundreds of plant species. Homologs of the bZIP transcription factor Atf1 are required for virulence in most pathogenic fungi, but the molecular basis for their involvement is largely unknown.We performed targeted gene deletion, expression analysis, biochemistry and pathogenicity assays to demonstrate that VdAtf1 governs pathogenesis via the regulation of nitrosative resistance and nitrogen metabolism in V. dahliae.VdAtf1 controls pathogenesis via the regulation of nitric oxide (NO) resistance and inorganic nitrogen metabolism rather than oxidative resistance and is important for penetration peg formation in V. dahliae. VdAtf1 affects ammonium and nitrate assimilation in response to various nitrogen sources. VdAtf1 may be involved in regulating the expression of VdNut1. VdAtf1 responds to NO stress by strengthening the fungal cell wall, and by causing over-accumulation of methylglyoxal and glycerol, which in turn impacts NO detoxification. We also verified that the VdAtf1 ortholog in Fusarium graminearum mediates nitrogen metabolism, suggesting conservation of this function in related plant pathogenic fungi.Our findings revealed new functions of VdAtf1 in pathogenesis, response to nitrosative stress and nitrogen metabolism in V. dahliae. The results provide novel insights into the regulatory mechanisms of the transcription factor VdAtf1 in virulence.

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Comprehensive Profiling of Lysine Acetylome in Baculovirus Infected Silkworm (Bombyx mori) Cells

Cited by 21 publications

References 74 publications

Orchestration of protein acetylation as a toggle for cellular defense and virus replication

Orchestration of protein acetylation as a toggle for cellular defense and virus replication

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

The bZIP transcription factor VdAtf1 regulates virulence by mediating nitrogen metabolism in Verticillium dahliae

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