Comprehensive multiplexed superfusion system enables physiological emulation in cell culture: exemplification by persistent circadian entrainment

Abstract

A multiplexed superfusion system enables dynamic in vitro physiological emulation, as demonstrated by persistent circadian entrainment.

A new tissue-agnostic microfluidic device to model physiology and disease: the lattice platform

“…14,15,17 Lastly, by integrating a pump mechanism with the base station, we forgo the need for external pneumatic infrastructure and various types of custom and off-the-shelf peristaltic pumps. 15,[38][39][40] This creates a 3D system that is more accessible, scalable, and compatible for various assays and user workflows. The culture plate is assembled from injection-molded plastics while most elements of the base station are made from commercially available components.…”

“…Affordable liquid handling and MPS solutions have been proposed but are not usable out of the box, 44 incompatible with current robotic handling setups, and require external tubing. 38,39 Currently, we are developing the LATTICE system to be compatible with device-agnostic automated laboratory scheduling software to increase the device's flexibility.…”