Comprehensive metabolomics profiling of seminal plasma in asthenozoospermia caused by different etiologies

Hou, Yuyang; He, Yuanping; Wang, Jingjie; Zhou, Yanfen; Ma, Bo; Li, Xin; Shi, Liang; Zhang, Qi

doi:10.1016/j.cca.2023.117530

Cited by 3 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 134 ], 26 proteins were significantly different in the seminal plasma of fertile donors when compared to men from couples with recurrent miscarriage, asthenozoospermic (AZS) men, and AZS men with varicocele. Comprehensive metabolomic profiling of seminal plasma in AZS men with varied etiologies associated metabolic anomalies of lactic acid, fructose, citric acid, glutamine, and pyruvic acid metabolic abnormalities with all the AZS groups, while each AZS etiology group had some unique metabolic changes [ 135 ]. Negative correlations of several metabolites including isopentenyl pyrophosphate, 2-phosphoglyceric acid, and γ-glutamyl-Se-methylselenocysteine with sperm deformities; and creatine riboside with sperm concentration were also reported [ 136 ].…”

Section: Sperm Defects Of Post-testicular Originmentioning

confidence: 99%

Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

Sutovsky,

Hamilton,

Zigo

et al. 2024

Biology of Reproduction

View full text Add to dashboard Cite

Conventional, brightfield-microscopic semen analysis provides important baseline information about sperm quality of an individual; however, it falls short of identifying subtle subcellular and molecular defects in cohorts of “bad”, defective human and animal spermatozoa with seemingly normal phenotypes. To bridge this gap, it is desirable to increase the precision of andrological evaluation in humans and livestock animals by pursuing advanced biomarker-based imaging methods. This review, spiced up with occasional classic movie references but seriously scholastic at the same time, focuses mainly on the biomarkers of altered male germ cell proteostasis resulting in post-testicular carryovers of proteins associated with ubiquitin-proteasome system. Also addressed are sperm redox homeostasis, epididymal sperm maturation, sperm-seminal plasma interactions and sperm surface glycosylation. Zinc ion homeostasis-associated biomarkers and sperm-borne components, including the elements of neurodegenerative pathways such as Huntington’s and Alzheimer’s disease, are discussed. Such spectrum of biomarkers, imaged by highly specific vital fluorescent molecular probes, lectins, and antibodies, reveals both obvious and subtle defects of sperm chromatin, DNA and accessory structures of the sperm head and tail. Introduction of next generation image-based flow cytometry into research and clinical andrology will soon enable the incorporation of machine and deep learning algorithms with the end point of developing simple, label-free methods for clinical diagnostics and high throughput phenotyping of spermatozoa in humans and economically important livestock animals.

show abstract

Section: Sperm Defects Of Post-testicular Originmentioning

confidence: 99%

Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

Sutovsky,

Hamilton,

Zigo

et al. 2024

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

DNA methylation patterns in patients with asthenospermia and oligoasthenospermia

Zhang,

Li,

Wang

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Background Spermatogenesis is a highly regulated and complex process in which DNA methylation plays a crucial role. This study aimed to explore the differential methylation profiles in sperm DNA between patients with asthenospermia (AS) and healthy controls (HCs), those with oligoasthenospermia (OAS) and HCs, and patients with AS and those with OAS. Results Semen samples and clinical data were collected from five patients with AS, five patients with OAS, and six age-matched HCs. Reduced representation bisulfite sequencing (RRBS) was performed to identify differentially methylated regions (DMRs) in sperm cells among the different types of patients and HCs. A total of 6520, 28,019, and 16,432 DMRs were detected between AS and HC, OAS and HC, and AS and OAS groups, respectively. These DMRs were predominantly located within gene bodies and mapped to 2868, 9296, and 9090 genes in the respective groups. Of note, 12, 9, and 8 DMRs in each group were closely associated with spermatogenesis and male infertility. Furthermore, BDNF, SMARCB1, PIK3CA, and DDX27; RBMX and SPATA17; ASZ1, CDH1, and CHDH were identified as strong differentially methylated candidate genes in each group, respectively. Meanwhile, the GO analysis of DMR-associated genes in the AS vs. HC groups revealed that protein binding, cytoplasm, and transcription (DNA-templated) were the most enriched terms in the biological process (BP), cellular component (CC), and molecular function (MF), respectively. Likewise, in both the OAS vs. HC and AS vs. OAS groups, GO analysis revealed protein binding, nucleus, and transcription (DNA-templated) as the most enriched terms in BP, CC, and MF, respectively. Finally, the KEGG analysis of DMR-annotated genes and these genes at promoters suggested that metabolic pathways were the most significantly associated across all three groups. Conclusions The current study results revealed distinctive sperm DNA methylation patterns in the AS vs. HC and OAS vs. HC groups, particularly between patients with AS and those with OAS. The identification of key genes associated with spermatogenesis and male infertility in addition to the differentially enriched metabolic pathways may contribute to uncovering the potential pathogenesis in different types of abnormal sperm parameters.

show abstract

Advancements and future trends in sperm detection techniques and male fertility assessment

WU,

WEI,

et al. 2024

Sci. Sin.-Vitae

View full text Add to dashboard Cite

Comprehensive metabolomics profiling of seminal plasma in asthenozoospermia caused by different etiologies

Cited by 3 publications

References 43 publications

Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly

DNA methylation patterns in patients with asthenospermia and oligoasthenospermia

Advancements and future trends in sperm detection techniques and male fertility assessment

Contact Info

Product

Resources

About