Comprehensive metabolomics identified lipid peroxidation as a prominent feature in human plasma of patients with coronary heart diseases

Abstract

Coronary heart disease (CHD) is a complex human disease associated with inflammation and oxidative stress. The underlying mechanisms and diagnostic biomarkers for the different types of CHD remain poorly defined. Metabolomics has been increasingly recognized as an enabling technique with the potential to identify key metabolomic features in an attempt to understand the pathophysiology and differentiate different stages of CHD. We performed comprehensive metabolomic analysis in human plasma from 28 human subjects with stable angina (SA), myocardial infarction (MI), and healthy control (HC). Subsequent analysis demonstrated a uniquely altered metabolic profile in these CHD: a total of 18, 37 and 36 differential metabolites were identified to distinguish SA from HC, MI from SA, and MI from HC groups respectively. Among these metabolites, glycerophospholipid (GPL) metabolism emerged as the most significantly disturbed pathway. Next, we used a targeted metabolomic approach to systematically analyze GPL, oxidized phospholipid (oxPL), and downstream metabolites derived from polyunsaturated fatty acids (PUFAs), such as arachidonic acid and linoleic acid. Surprisingly, lipids associated with lipid peroxidation (LPO) pathways including oxidized PL and isoprostanes, isomers of prostaglandins, were significantly elevated in plasma of MI patients comparing to HC and SA, consistent with the notion that oxidative stress-induced LPO is a prominent feature in CHD. Our studies using the state-of-the-art metabolomics help to understand the underlying biological mechanisms involved in the pathogenesis of CHD; LPO metabolites may serve as potential biomarkers to differentiation MI from SA and HC.

Metabolomics Study of the Biochemical Changes in the Plasma of Myocardial Infarction Patients

“…Interestingly, we observed glycerophospholipid metabolism emerged as the most significantly disturbed pathway, which was consistent with previous findings (Lu et al, 2017). Consequently, the identified metabolites, especially phosphatidylserine, linoleamidoglycerophosphate choline, Lyso-PC (C18:2), Lyso-PC (C16:0), and Lyso-PC (C18:1), may be better metabolites to forecast the risk for MI patients.…”

Phospholipids and L-Carnitine Screening in Children with Congenital Heart Diseases Undergoing Surgical Correction

“…However, Ohkawaa et al Found that marked increase in Sphingomyelin level with acute myocardial infarction in comparison to normal population (21). Also, Lu J and colleagues, noticed that marked increase in plasma lipids level in acute coronary syndrome patients in comparison to normal population (22). The present study showed, statistically significant lower serum L-carnitine levels in CHD when compared with the normal children.…”

Plasma metabolites mediate the effect of HbA1c on incident cardiovascular disease

“…DeRatt et al found that a high plasma cystathionine concentration in stable angina pectoris was associated with higher glucose, and phenylalanine concentrations due to greater catabolism . Furthermore, glycerophospholipid metabolism and butanoate metabolism are also relevant to oxidative stress, and glycerophospholipid metabolism has been confirmed to be associated with CVD risk …”