Comprehensive metabolomic, proteomic and physiological analyses of grain yield reduction in rice under abrupt drought–flood alternation stress

Abstract

Abrupt drought-flood alternation (T1) is a meteorological disaster that frequently occurs during summer in southern China and the Yangtze river basin, often causing a significant loss of rice production. In this study, the response mechanism of yield decline under abrupt drought-flood alternation stress at the panicle differentiation stage was analyzed by looking at the metabolome, proteome as well as yield and physiological and biochemical indexes. The results showed that drought and flood stress caused a decrease in the yield of rice at the panicle differentiation stage, and abrupt drought-flood alternation stress created a synergistic effect for the reduction of yield. The main reason for the decrease of yield per plant under abrupt drought-flood alternation was the decrease of seed setting rate. Compared with CK0 (no drought and no flood), the net photosynthetic rate and soluble sugar content of T1 decreased significantly and its hydrogen peroxidase, superoxide dismutase, peroxidase activity increased significantly. The identified differential metabolites and differentially expressed proteins indicated that photosynthesis metabolism, energy metabolism pathway and reactive oxygen species response have changed strongly under abrupt drought-flood alteration stress, which are factors that leads to the rice grain yield reduction.

Proteomic Profiling Reveals Differentially Expressed Proteins Associated with Amylose Accumulation During Rice Grain Filling

“…Proteomics pro ling has been approved as a powerful molecular strategy that has been widely implemented in dissecting the molecular basis of various biological processes in living organisms including plants. However, the experimental system and procedure of the employed proteomics pro ling approach greatly affect the powerful and e ciency of proteomic pro ling in dissecting the molecular mechanisms of a biological process [18][19][20][21][22][23][24][25][26]. In rice, seed development is a complex biological process that is greatly affect grain yield and quality and is governed by complex regulatory networks comprising numerous transcription factors [27].…”

Comparative Quantitative Proteomic Analysis Reveals Differentially Expressed Proteins Involved in Grain-Filling Rate of Rice at the Early Ripening Stage

“…Analysis of proteomics pro les has been demonstrated as a powerful and e cient molecular tool in exploring molecular mechanisms underlying various biological processes in living organisms. However, the e ciency of proteomic analysis in identi cation of proteins underlying a given biological process greatly depends on the applied proteomics analysis procedure and the experimental system [28][29][30][31][32][33]. Although proteomic analyses have been widely employed to dissect the molecular basis of variable biological processes in plants, in particular stress tolerance, [29,32,[34][35][36][37][38][39]), to the best of our knowledge this is the rst comprehensive study conducted to dissect proteins involved in grain lling rate of rice.…”

“…However, the e ciency of proteomic analysis in identi cation of proteins underlying a given biological process greatly depends on the applied proteomics analysis procedure and the experimental system [28][29][30][31][32][33]. Although proteomic analyses have been widely employed to dissect the molecular basis of variable biological processes in plants, in particular stress tolerance, [29,32,[34][35][36][37][38][39]), to the best of our knowledge this is the rst comprehensive study conducted to dissect proteins involved in grain lling rate of rice. Seed development in rice is a complex biological process that directly impact potential yield and is regulated by complex regulatory networks involving several transcription factors [40].In the current study, two rice cultivars, i.e., Xiangzaoxian 24 and Xiangzaoxian 42, that possess contrasting phenotypes in grain lling rate during the early ripening period were employed in identi cation of grain lling differentially expressed proteins.…”

Proteomic profiling reveals differentially expressed proteins associated with amylose accumulation during rice grain filling

“…Proteomics pro ling has been approved as a powerful molecular strategy that has been widely implemented in dissecting the molecular basis of various biological processes in living organisms including plants. However, the experimental system and procedure of the employed proteomics pro ling approach greatly affect the power and e ciency of proteomic pro ling in dissecting the molecular mechanisms of a biological process [19][20][21][22][23][24][25][26][27][28]. In rice, seed development is a complex biological process that greatly affects grain yield and quality and is governed by complex regulatory networks comprising numerous transcription factors [29].…”