Comprehensive mechanistic insights into the citrus genetics, breeding challenges, biotechnological implications, and omics-based interventions

Gill, Komaljeet; Kumar, Pankaj; Kumar, Anshul; Kapoor, Bhuvnesh; Sharma, Rajnish; Joshi, Ajay Kumar

doi:10.1007/s11295-022-01544-z

Cited by 11 publications

(6 citation statements)

References 167 publications

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“…Anyway, the studying genome is not a wild type. All of these mandarins have considerable introgression of segments from pummelo which creates regions of high heterozygosity compared to regions of mandarin only origin (Ollitrault et al 2011; Gill et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing and Variant Discovery ofCitrus reticulata‘Kinnow’ from Pakistan

Jabeen

Saif

Haq

et al. 2022

Preprint

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Citrus is a source of many nutritional and medicinal advantages, which is cultivated worldwide with major citrus groups of sweet oranges, mandarins, grapefruits, kumquats, lemons and limes. Pakistan produces all of its major citrus groups with mandarin (Citrus reticulata) being the prominent group that includes local commercial cultivars such as Feutral's Early, Dancy, Honey and Kinnow. The present study was designed to understand the genetic architecture of this unique variety of Citrus reticulata -'Kinnow'. The whole-genome resequencing and variant calling was performed to map the genomic variability that might be responsible for its particular characteristics like taste, seedlessness, juice content, thickness of peel and shelf-life. A total of 139,436,350 raw sequence reads using Illumina platform were generated with 20.9 Gb data in Fastq format having 98% effectiveness and 0.2% base call error rate. Overall, a total of 3,503,033 SNPs, 176,949 MNPs, 323,287 INS and 333,083 DEL were identified using the GATK4 variant calling pipeline against Citrus clementina as a reference genome. Further, g:Profiler bioinformatics tool was applied for annotating the newly found variants, harbor genes/transcripts and their involved pathways. A total of 73,864 transcripts harbors 4,336,352 variants, most of the observed variants were predicted in non-coding regions and 1,009 transcripts were found well annotated by different databases. Out of the total aforementioned transcripts, 588 involved in biological processes, 234 in molecular functions and 167 transcripts involved in cellular components in Citrus reticulata. In a nutshell, 18,153 high-impact variants and 216 genic-variants found in the current study which may be used for marker-assisted breeding programs of 'Kinnow' to identify this particular variety among others and to propagate its valued traits to improve the contemporary citrus varieties as well.

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Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing and Variant Discovery ofCitrus reticulata‘Kinnow’ from Pakistan

Jabeen

Saif

Haq

et al. 2022

Preprint

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“…Hoy en día los más importantes incluyen la naranja dulce (C. sinensis), naranja agría (C. aurantium L.), toronja (C. paradisi), naranja pomelo (C. máxima), mandarina (C. reticulata), lima (C. aurantifolia), y limón (C. lemon) [9] .…”

Section: Especies De Cítricos De Importanciaunclassified

“…Predominan variedades cultivadas obtenidas por cruzamientos tradicionales y hay algunas que han sido modificadas genéticamente que resultaron exitosas en la expresión de características deseables en la fruta, fenotipo de la planta y resistencia. Sin embargo, estos procesos de mejoramiento son complejos y largos en tiempo pues el género presenta: alta heterogeneidad genética, incompatibilidad propia y cruzada, poliembrionía, esterilidad y una fase juvenil larga [9] . Aun así, la transformación genética funciona para la mejora genética de los cítricos ya que se basa en la introducción de rasgos específicos en genotipos conocidos, sin alterar los antecedentes genéticos de elite [10] y, sobre todo, controlando las limitantes descritas anteriormente y por ende mejorando la eficiencia de las transformaciones y la posibilidad de regenerar plantas completas al lograr combinar las técnicas clásicas de botánica y citogenética con los avances de la genética, la biología molecular y la biotecnología.…”

Section: Variedades Mejoradasunclassified

El impacto de Huanglongbing, HLB, en la producción mundial de cítricos

Pineda Cagua,

Hernández Mendoza,

Hernández Delgado

et al. 2024

Rev. Bol. Quim.

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El cultivo de naranja se originó en el hemisferio oriental, principalmente en China, y luego se extendió por todo el mundo como fruta comercial. En 2022, la producción mundial de naranjas aumentó a un total de 49 millones de toneladas métricas. Los principales productores incluyen Brasil, China, la UE, México y Estados Unidos. Sin embargo, la industria de los cítricos enfrenta desafíos debido a la enfermedad de Huanglongbing (HLB). Los investigadores están explorando modificaciones genéticas para lograr tolerancia. Esta revisión explora el impacto de la enfermedad, las especies de cítricos de importancia, las variedades mejoradas y la evolución global del HLB.

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“…Oryza sativa L. (rice) and Citrus reticulata Blanco (citrus) are globally significant food crops and economic crops with wide distribution across hundreds of countries [ 1 , 2 ]. They have a rich history of cultivation and consumption in China.…”

Section: Introductionmentioning

confidence: 99%

Residues and Dietary Risk Assessment of Prohexadione-Ca and Uniconazole in Oryza sativa L. and Citrus reticulata Blanco by Liquid Chromatography-Tandem Mass Spectrometry

Luo

et al. 2023

Molecules

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A simple and sensitive method for the simultaneous quantitation of prohexadione-Ca and uniconazole in the field experiment of Oryza sativa L. and Citrus reticulata Blanco was established using solid-phase extraction (SPE) with polymer anion exchange (PAX) and Florisil followed by LC-MS/MS. The method demonstrated excellent linearity (R2 > 0.999 0), trueness (recoveries between 95~105%), precision (CVs between 0.8~12%), sensitivity, and repeatability (LOQ of 0.05 and 0.01 mg/kg, respectively). Residue tests were conducted in the field at 12 representative sites in China, revealing final concentrations of prohexadione-Ca and uniconazole in brown rice, rice hull, and rice straw to be below 0.05 mg/kg, while in whole citrus fruit and citrus pulp, they were below 0.01 mg/kg. These were below the maximum residue limits specified in China. The chronic dietary risks of prohexadione-Ca and uniconazole in rice crops and citrus fruits were calculated to be 0.48% and 0.91%, respectively. Our research suggests that the chronic risk associated with the daily consumption of rice crops and citrus fruit at the recommended dosage is acceptable.

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Comprehensive mechanistic insights into the citrus genetics, breeding challenges, biotechnological implications, and omics-based interventions

Cited by 11 publications

References 167 publications

Whole-Genome Sequencing and Variant Discovery ofCitrus reticulata‘Kinnow’ from Pakistan

Whole-Genome Sequencing and Variant Discovery ofCitrus reticulata‘Kinnow’ from Pakistan

El impacto de Huanglongbing, HLB, en la producción mundial de cítricos

Residues and Dietary Risk Assessment of Prohexadione-Ca and Uniconazole in Oryza sativa L. and Citrus reticulata Blanco by Liquid Chromatography-Tandem Mass Spectrometry

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