Comprehensive mapping of neurotransmitter networks by MALDI–MS imaging

Shariatgorji, Mohammadreza; Nilsson, Anna; Fridjonsdottir, Elva; Vallianatou, Theodosia; Källback, Patrik; Katan, Luay; Sävmarker, Jonas; Mantas, Ioannis; Zhang, Xiaoqun; Bézard, Erwan; Svenningsson, Per; Odell, Luke R.; Andrén, Per E.

doi:10.1038/s41592-019-0551-3

Cited by 170 publications

(189 citation statements)

References 45 publications

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“…Regarding the MSI data, we identified all the previously characterized metabolites (33). We were able to pinpoint the peak of TCP itself, which did not differ significantly between the two genotypes ( Figure S3; Table S4).…”

Section: Statistical Analysis J O U R N a L P R E -P R O O Fmentioning

confidence: 94%

“…Fresh frozen brain tissue sections collected for MALDI-MSI were thaw-mounted on indium tin oxide coated glass slides (Bruker Daltonics, Bremen, Germany). For MALDI-MSI of neurotransmitters and metabolites on-tissue derivatization was performed according to a previously published protocol (33). For details, see SI.…”

Section: J O U R N a L P R E -P R O O F Maldi-msi Of Neurotransmittermentioning

confidence: 99%

“…We therefore explored whether the behavioral genotype difference was dependent upon alterations in the levels of tyramine, monoamines and/or their metabolites. We used a recently developed MALDI-MSI method to quantify tyramine, monoamines and their metabolites after TCP in WT and TAAR1-KO mice (33). To avoid possible fluctuations of monoamine metabolites after MAO inhibition, we administered TCP chronically (Figure 2a).…”

Section: Statistical Analysis J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

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TAAR1-Dependent and -Independent Actions of Tyramine in Interaction With Glutamate Underlie Central Effects of Monoamine Oxidase Inhibition

Mantas

Vallianatou

Yang

et al. 2021

Biological Psychiatry

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Section: Statistical Analysis J O U R N a L P R E -P R O O Fmentioning

confidence: 94%

Section: J O U R N a L P R E -P R O O F Maldi-msi Of Neurotransmittermentioning

confidence: 99%

Section: Statistical Analysis J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

See 1 more Smart Citation

TAAR1-Dependent and -Independent Actions of Tyramine in Interaction With Glutamate Underlie Central Effects of Monoamine Oxidase Inhibition

Mantas

Vallianatou

Yang

et al. 2021

Biological Psychiatry

Self Cite

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“…Additional derivatization methods have been developed since the initial report on primary amines only. For example, fluoromethylpyridinium-based materials Mass Spectrometry Imaging of Neurotransmitters DOI: http://dx.doi.org /10.5772/intechopen.93835 are reactive with phenolic hydroxyl and/or primary or secondary amines, which expand the potential range of NTs that can be detected [87]. Charge-tagging using 2-(4-bromophenyl)-4,6-diphenylpyranylium (Br-TPP) results in distinctive isotopic distributions in the mass spectrum, making it easier to identify derivatized species from other potential species [88].…”

Section: Derivatization Strategiesmentioning

confidence: 99%

“…The method used an in-house synthesized stable isotope and correlated the results with LC-MS/MS [98]. Lastly, there is one example that specifically focused on comprehensive mapping of NTs in Parkinson's disease lesioned mouse brain and demonstrated quantitation of dopamine using a stable isotope [87]. All of the drug molecules listed in this paragraph are above the general size range that NTs and metabolites fall within, ranging from 400 to 800 Da, but present possible future avenues of research for the NT-focused methods discussed in this chapter.…”

Section: Quantitationmentioning

confidence: 99%

Mass Spectrometry Imaging of Neurotransmitters

Stumpo¹

2021

Mass Spectrometry in Life Sciences and Clinical Laboratory

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Mass spectrometry imaging (MSI) is a powerful analytical method for the simultaneous analysis of hundreds of compounds within a biological sample. Despite the broad applicability of this technique, there is a critical need for advancements in methods for small molecule detection. Some molecular classes of small molecules are more difficult than others to ionize, e.g., neurotransmitters (NTs). The chemical structure of NTs (i.e., primary, secondary, and tertiary amines) affects ionization and has been a noted difficulty in the literature. In order to achieve detection of NTs using MSI, strategies must focus on either changing the chemistry of target molecules to aid in detection or focus on new methods of ionization. Additionally, even with new strategies, the issues of delocalization, chemical background noise, and ability to achieve high throughput (HTP) must be considered. This chapter will explore previous and up-and-coming techniques for maximizing the detection of NTs.

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Advances in multimodal mass spectrometry for single‐cell analysis and imaging enhancement

Croslow,

Trinklein,

Sweedler

2024

FEBS Letters

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Multimodal mass spectrometry (MMS) incorporates an imaging modality with probe‐based mass spectrometry (MS) to enable precise, targeted data acquisition and provide additional biological and chemical data not available by MS alone. Two categories of MMS are covered; in the first, an imaging modality guides the MS probe to target individual cells and to reduce acquisition time by automatically defining regions of interest. In the second category, imaging and MS data are coupled in the data analysis pipeline to increase the effective spatial resolution using a higher resolution imaging method, correct for tissue deformation, and incorporate fine morphological features in an MS imaging dataset. Recent methodological and computational developments are covered along with their application to single‐cell and imaging analyses.

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Comprehensive mapping of neurotransmitter networks by MALDI–MS imaging

Cited by 170 publications

References 45 publications

TAAR1-Dependent and -Independent Actions of Tyramine in Interaction With Glutamate Underlie Central Effects of Monoamine Oxidase Inhibition

TAAR1-Dependent and -Independent Actions of Tyramine in Interaction With Glutamate Underlie Central Effects of Monoamine Oxidase Inhibition

Mass Spectrometry Imaging of Neurotransmitters

Advances in multimodal mass spectrometry for single‐cell analysis and imaging enhancement

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