Comprehensive Liquid Chromatography and Other Liquid-Based Comprehensive Techniques Coupled to Mass Spectrometry in Food Analysis

Cacciola, Francesco; Donato, Paola; Sciarrone, Danilo; Dugo, Paola; Mondello, Luigi

doi:10.1021/acs.analchem.6b04370

Cited by 48 publications

(20 citation statements)

References 130 publications

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“…Unlike other previously investigated samples where a phenolic group of compounds was clearly predominant [23][24][25] demonstrated that they can also be run under HILIC conditions with satisfactory results [26]. For this reason, in this work, the performance of the PEG column was studied under both separation modes, as RP × RP has also previously shown relatively good performance in phenolic compounds analysis [13,17]. conditions, the PEG column did not produce satisfactory retention of the studied compounds.…”

Section: Separation Methods Optimizationmentioning

confidence: 78%

“…LC × LC is based on the coupling of two independent separation mechanisms that allow significant improvements on resolving power and peak capacity [12]. By using this on-line approach, the entire sample is subjected to two independent separation mechanisms continuously; although different combinations between separation mechanisms may be applied, the one involving hydrophilic interaction chromatography (HILIC) coupled to reversed phase (RP) separations has shown a very good potential for polyphenols analysis [13]. In any case, the application of this coupling is not straightforward due to multiple factors that should be optimized [14][15][16], being one of the most important the transfer from the first dimension ( 1 D) eluent to the second dimension ( 2 D) continuously, due to solvent incompatibility.…”

Section: Introductionmentioning

confidence: 99%

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Profiling of Vitis vinifera L. canes (poly)phenolic compounds using comprehensive two-dimensional liquid chromatography

Montero

Sáez

Baer

et al. 2018

Journal of Chromatography A

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Grapevine canes, a pruning-derived by-product, possess a great amount of bioactive (poly)phenolic compounds belonging to different chemical classes, thus, having a good potential for further valorization. However, in order to properly design valorization strategies, the precise chemical composition of this material has to be known. Up to now, this chemical characterization has been based on analysis of different groups of components individually, due to difficulties related to their huge chemical variability. In this work, a comprehensive two-dimensional liquid chromatography-based method (LC×LC) is developed to obtain the profiles of (poly)phenolic compounds present in grapevine canes from several varieties. Three different set-ups have been tested and compared; the combination of diol and C columns produced the best results, allowing the characterization of the (poly)phenolic profile in around 80min. This way, 81 different components were detected in the samples; most of them could be tentatively assigned using the information provided by the DAD and MS detectors employed. Indeed, it has been possible to detect in a single run components belonging to stilbenoids, procyanidins and prodelphinidins of varying degrees of polymerization, some of them not formerly described in this natural source. The method has shown extremely good separation capabilities, and is characterized by high effective peak capacity (842) and orthogonality (A=78%). The obtained results demonstrate that Vitis vinifera L. canes may retain a great potential to be used as an underexploited natural source of bioactive compounds, with potential applications in different fields.

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Section: Separation Methods Optimizationmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Profiling of Vitis vinifera L. canes (poly)phenolic compounds using comprehensive two-dimensional liquid chromatography

Montero

Sáez

Baer

et al. 2018

Journal of Chromatography A

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“…As viable alternative for enhancing the overall separation capability, comprehensive 2D‐LC (LC × LC) has been far employed for phenol determination in many food and natural products as witnessed by several recent research and review articles [21–34]. It was already reported that LC × LC minimize matrix interferences at the detector, because of the higher resolving power, viz .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of matrix effect in one‐dimensional and comprehensive two‐dimensional liquid chromatography for the determination of the phenolic fraction in extra virgin olive oils

Arena

Cacciola

Rigano

et al. 2020

J of Separation Science

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Olea europaea, meaning “European olive,” is a small tree belonging to the family Oleaceae, occurring in the Mediterranean Basin. Olive oil is an essential component of a balanced diet because of its nutritional value. Among micronutrients, phenolic compounds did show important beneficial effects for human health. The majority of the research studies on the phenol content are carried out by liquid chromatography combined to photodiode array and/or mass spectrometry detection; however, because of matrix complexity, one‐dimensional liquid chromatography cannot be sometimes sufficient to obtain rewarding separations, requiring more advanced analytical techniques. In this work, comprehensive two‐dimensional liquid chromatography, incorporating RP‐Amide and C18 stationary phases, in the first and second dimension, respectively, both under reversed phase conditions, was investigated for the determination of the phenolic fraction in extra virgin olive oil samples. As far as detection is concerned, triple quadrupole mass spectrometry was employed under multi reaction monitoring mode offering superior selectivity and sensitivity. The reduction of matrix effects, when using comprehensive two‐dimensional liquid chromatography with respect to conventional one‐dimensional liquid chromatography, was assessed by comparing the slopes of calibration curves built from standard solutions and spiked olive oil samples.

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“…The emergence of comprehensive two‐dimensional LC (LC × LC) that combines two separation mechanisms has provided an alternative platform for high resolution analysis of complex real‐world samples . In comparison with conventional 1D‐LC, LC × LC provides higher resolving power (as a result of a multiplicative increase in peak capacity), in addition to structured chromatogram where components are spread out in 2D space according to chemical specificity of retention patterns .…”

Section: Introductionmentioning

confidence: 99%

“…hydrophobicity, polarity, size and charge) to achieve a potentially “orthogonal” separation in LC × LC possess some technical difficulties. For instance, a combination of normal phase (NP) and reversed‐phase (RP) can result in mobile phase immiscibility and lead to precipitation of buffers or salts . Off‐line LC × LC techniques which offered more simplicity have been frequently exploited.…”

Section: Introductionmentioning

confidence: 99%

Untargeted profiling of Glycyrrhiza glabra extract with comprehensive two‐dimensional liquid chromatography‐mass spectrometry using multi‐segmented shift gradients in the second dimension: Expanding the metabolic coverage

et al. 2018

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Metabolic profiling of Glycyrrhiza glabra using comprehensive two-dimensional liquid chromatography (LC × LC) coupled with photodiode array (PDA) and mass spectrometry (MS) detection is described. The separation was conducted under reversed-phase conditions, using a combination of first dimension ( D) 150 mm microbore cyano column utilising 2.7 μm diameter (d ) particles, and second dimension ( D) 50 mm superficially porous octadecylsilica column with 2.7 μm d particles. A multi-segmented shift gradient (MSG) for the D separation was developed, and the orthogonality achieved was compared with other modes of gradients, such as full in-fraction, and shift gradient systems. Results demonstrated a significant expansion of metabolic coverage using MSG in D, providing the highest measure of orthogonality compared to other gradient modes. Compound identifications were performed by employing complementary data from PDA and MS detection, with reference to structural group-type distribution in 2D space. A total of ca. 120 compounds were detected, and among them 37 were tentatively identified, distributed over the chemical families of glycosylated flavanones, triterpene saponins, and others. In comparison with one-dimensional LC, the total number of compounds detected was ca. 2-fold greater when LC × LC was employed. To the best of our knowledge, this is the first demonstration of the MSG mode in LC × LC, representing a powerful strategy to expand the metabolic coverage for analysis of plant-derived extracts, containing a multitude of different phytochemical classes.

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Comprehensive Liquid Chromatography and Other Liquid-Based Comprehensive Techniques Coupled to Mass Spectrometry in Food Analysis

Cited by 48 publications

References 130 publications

Profiling of Vitis vinifera L. canes (poly)phenolic compounds using comprehensive two-dimensional liquid chromatography

Profiling of Vitis vinifera L. canes (poly)phenolic compounds using comprehensive two-dimensional liquid chromatography

Evaluation of matrix effect in one‐dimensional and comprehensive two‐dimensional liquid chromatography for the determination of the phenolic fraction in extra virgin olive oils

Untargeted profiling of Glycyrrhiza glabra extract with comprehensive two‐dimensional liquid chromatography‐mass spectrometry using multi‐segmented shift gradients in the second dimension: Expanding the metabolic coverage

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