Comprehensive lipidomic analysis of milk polar lipids using ultraperformance supercritical fluid chromatography-mass spectrometry

Buffalo is the second source of milk in the world, and its milk is rich in nutritive components. It is well-known that breed influences milk composition. This work aimed to compare the detailed milk composition of three buffalo breeds (Murrah, Nili-Ravi, and Mediterranean) housed under the same environmental conditions. Mediterranean buffalo milk showed a significantly higher content of fat, protein, and some fatty acids. Moreover, the milk from the Mediterranean breed was characterized by the highest content of sphingomyelin (SM), cholesterol, and lanosterol. However, the Murrah buffalo milk contained the highest amount of total unsaturated fatty acids, phosphatidylinositol, and whey proteins. Furthermore, the Nili-Ravi buffalo milk was characterized by the highest content of total saturated fatty acids, phosphatidylglycerol, squalene, lathosterol, stigmasterol, beta-sitosterol, and casein fractions. Nevertheless, the lactose and amino acid profiles of the milk remained almost similar across the three buffalo breeds. The generated results in this study enable a comprehensive understanding of the milk constituent variability that is linked to buffalo breeds, which may support the acquirement of essential scientific knowledge on milk ingredient–processing interactions that will offer a foundation of knowledge for Chinese dairy processors in terms of milk processability and innovation.

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“…This result is different from that of Holstein milk [ 31 ], which may be related to the breed and feeding method.…”

Section: Resultsmentioning

confidence: 61%

Effect of Buffalo Breed on the Detailed Milk Composition in Guangxi, China

Abdel-Hamid

Huang

et al. 2023

Foods

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“…The Acquity UPSFC system (Waters, Milford, USA) was equipped with an HPLC pump and coupled to a Xevo G2-S Q-TOF-MS mass spectrometer, according to our previous study. 25 The results of the m / z values and fragment information with calculated extract masses were identified by using the software of Progenesis QI (Nonlinear Dynamics, Waters, Milford, MA, USA) equipped with two databases.…”

Section: Methodsmentioning

confidence: 99%

“…Phospholipids of human and cow's milk samples were extracted and purified by solid phase extraction following our previous study. 25 The phospholipids were separated from an Acquity UPC 2 ™ BEH Column (1.7 μm, 3 mm × 150 mm, Waters, Milford, USA) at 55 °C. The Acquity UPSFC system (Waters, Milford, USA) was equipped with an HPLC pump and coupled to a Xevo G2-S Q-TOF-MS mass spectrometer, according to our previous study.…”

Section: Phospholipid Compositions Analysismentioning

confidence: 99%

Comparison of lipid structure and composition in human or cow's milk with different fat globules by homogenization

Zhao

Zhang

et al. 2023

Food Funct.

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“…More recently, Jiang et al. [143] employed a novel method for the high‐throughput analysis of gangliosides in milk samples, using ultra‐performance supercritical fluid chromatography equipped with QTOF MS. In almost all previously described articles, standards of GM3 and GD3 were used.…”

Section: Milk Gangliosidesmentioning

confidence: 99%

“…More recently, modern techniques based on LC [127,128,142], HPLC [126], or UPLC [125,133,136] hyphenated to MS [125,126,129,142] or tandem MS [125,133,136,143] were used for their advantage of generating structural information on the ceramide moiety, polysaccharide chains and fatty acids. Hence, an LC MS/MS with a pneumatic ESI technique was developed and validated for the first time by Sørensen et al [127].…”

Section: Milk Gangliosidesmentioning

confidence: 99%

Ganglioside analysis in body fluids by liquid‐phase separation techniques hyphenated to mass spectrometry

et al. 2022

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The expression of gangliosides in central nervous system is a few times higher than in the extraneural tissue, a characteristic highlighting their major role at this level. Although in very low amounts, gangliosides are ubiquitously distributed in body fluids too, where, depending on many factors, including pathological states, their composition fluctuates, thus having diagnostic value. Ganglioside investigation in biological fluids, which, except for cerebrospinal fluid (CSF), may be sampled noninvasively, was for years impeded by the limited sensitivity of the analytical instrumentation available in glycomics. However, because the last decade has witnessed significant developments in biological mass spectrometry (MS) and the hyphenated separation techniques, marked by a major increase in sensitivity, reproducibility, and data reliability, ganglioside research started to be focused on biofluid analysis by separation techniques coupled to MS. In this context, our review presents the achievements in this emerging field of gangliosidomics, with a particular emphasis on modern liquid chromatography (LC), thin-layer chromatography, hydrophilic interaction LC, and ion mobility

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Comprehensive lipidomic analysis of milk polar lipids using ultraperformance supercritical fluid chromatography-mass spectrometry

Cited by 21 publications

References 36 publications

Effect of Buffalo Breed on the Detailed Milk Composition in Guangxi, China

Effect of Buffalo Breed on the Detailed Milk Composition in Guangxi, China

Comparison of lipid structure and composition in human or cow's milk with different fat globules by homogenization

Ganglioside analysis in body fluids by liquid‐phase separation techniques hyphenated to mass spectrometry

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