Comprehensive lipid profiling in the Mediterranean mussel (Mytilus galloprovincialis) using hyphenated and multidimensional chromatography techniques coupled to mass spectrometry detection

Donato, Paola; Micalizzi, Giuseppe; Oteri, Marianna; Rigano, Francesca; Sciarrone, Danilo; Dugo, Paola; Mondello, Luigi

doi:10.1007/s00216-018-1045-3

Cited by 36 publications

(16 citation statements)

References 43 publications

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“…However, working at a lipid molecular species level by untargeted lipidomics showed changes in the composition of polar lipids of the spat, offering the most complete overview of the spat lipidome. PC and PE are the main polar lipid components in bivalves [62,64,65,67,88,100] principally observed in the OUT samples (Fig 5B), possibly as a result of seasonality, as an increase of plasmalogens on mussels between winter/spring and summer conditions was found by Facchini, Losito [62]. When spat were fed I. galbana, PC(38:6) was the most abundant PC, as this might have incorporated a DHA molecule in the sn-2 position.…”

Section: Influence Of Dietary Treatments On Membrane and Polar Lipidsmentioning

confidence: 78%

“…searching the lipid dataset versus LIPID MAPS (www.lipidmaps.org), HMDB (www.hmdb. ca), Metlin (www.metlin.scripps.edu) and an "in house" bivalve lipid database built from recent bivalve lipidomics studies [60][61][62][63][64][65][66][67][68][69]. Contaminants were manually removed from the peak intensity table (PIT) generated from this process.…”

Section: A(%) B(%)mentioning

confidence: 99%

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Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

et al. 2020

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Blue mussels (Mytilus edulis L. 1758) are important components of coastal ecosystems and in the economy of rural and coastal areas. The understanding of their physiological processes at key life stages is important both within food production systems and in the management of wild populations. Lipids are crucial molecules for bivalve growth, but their diversity and roles have not been fully characterised. In this study, traditional lipid profiling techniques, such as fatty acid (FA) and lipid class analysis, are combined to untargeted lipidomics to elucidate the lipid metabolism in newly settled spat fed on a range of diets. The evaluated diets included single strains treatments (Cylindrotheca fusiformis CCAP 1017/2 -CYL, Isochrysis galbana CCAP 927/1-ISO, Monodopsis subterranean CCAP 848/1 -MONO, Nannochloropsis oceanica CCAP 849/10-NANNO) and a commercial algae paste (SP). Spat growth was influenced by the diets, which, according to their efficacy were ranked as follows: ISO>NANNO/CYL>SP>MONO. A higher triacylglycerols (TG) content, ranging from 4.23±0.82 μg mg ashfree Dry weight (DW) -1 at the beginning of the trial (T0) to 51 OPEN ACCESS Citation: Laudicella VA, Beveridge C, Carboni S, Franco SC, Doherty MK, Long N, et al. (2020) Lipidomics analysis of juveniles' blue mussels (Mytilus edulis L. 1758), a key economic and ecological species. PLoS ONE 15(2): e0223031.

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Section: Influence Of Dietary Treatments On Membrane and Polar Lipidsmentioning

confidence: 78%

Section: A(%) B(%)mentioning

confidence: 99%

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

et al. 2020

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“…The observed changes in lipid isomer compositions in diseased states potentially indicate altered expression or activities of enzymes involved in lipid metabolism. In human cells, for instance, the biosynthesis of mono-unsaturated fatty acids (MUFAs) was catalyzed by two 16:1_18:1(9) 16:1_18:1 (11) 16:0_18:1(9) 16:0_18:1 1118:2_18:1(9) 18:2_18:1 1118:1_18:1(9) 18:1_18:1 1118:0_18:1(9) 18:0_18:1(11) 20:3_18:1(9) 20:3_18:1(11)…”

Section: Discussionmentioning

confidence: 99%

“…An increasing number of researches have revealed a correlation between lipid alterations and various diseases due to lipid reprogramming [4][5][6][7][8][9] . Currently, mass spectrometry (MS) has become the method of choice for lipid analysis, offering label-free detection at high sensitivity and structural characterization capability [10][11][12][13][14][15][16][17][18] . However, large-scale lipid analysis with a comprehensive capability of revealing all levels of structure information still represents a significant analytical challenge for lipidomics [19][20][21] .…”

mentioning

confidence: 99%

Large-scale lipid analysis with C=C location and sn-position isomer resolving power

Cao¹,

Cheng²,

Yang³

et al. 2020

Nat Commun

129

183

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Lipids play a pivotal role in biological processes and lipid analysis by mass spectrometry (MS) has significantly advanced lipidomic studies. While the structure specificity of lipid analysis proves to be critical for studying the biological functions of lipids, current mainstream methods for large-scale lipid analysis can only identify the lipid classes and fatty acyl chains, leaving the C=C location and sn-position unidentified. In this study, combining photochemistry and tandem MS we develop a simple but effective workflow to enable large-scale and near-complete lipid structure characterization with a powerful capability of identifying C=C location(s) and sn-position(s) simultaneously. Quantitation of lipid structure isomers at multiple levels of specificity is achieved and different subtypes of human breast cancer cells are successfully discriminated. Remarkably, human lung cancer tissues can only be distinguished from adjacent normal tissues using quantitative results of both lipid C=C location and sn-position isomers.

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“…Working at single lipid species level allowed to capture changes in the composition of polar lipids of the spat, which were not observed from lipid class analysis due to the different separation employed in the two techniques. PC and PE are the main polar lipid components in bivalves [62, 64, 65, 67, 87, 99]. Bivalves have well-conserved PC structures, with a SAFA (16:0 and minor 18:0) in sn-1 position and an unsaturated PUFA (EPA or DHA) on sn-2 [99].…”

Section: Discussionmentioning

confidence: 99%

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulisL. 1758), a key economic and ecological species

Laudicella

Beveridge

Carboni

et al. 2019

Preprint

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Blue mussels (Mytilus edulis L.) are important components of coastal ecosystems functioning through benthopelagic coupling and ecosystem engineering. At the same time, mussel production is central in the economy of coastal areas. Therefore, understanding their nutritional, physiological and metabolic processes at key life stages is important for their management, both within food production systems and in wild populations.Lipids are crucial molecules for bivalve growth, but their diversity and roles have been considered from fatty acid (FA) perspective. In this paper, we applied lipidomics to bivalve nutrition. Lipidomics provides a holistic perspective on lipid patterns; by examining the lipidome, important physiological information can be acquired. Here, we use controlled laboratory experiments to elucidate the responses to changes in the diet of newly settled mussels juveniles, one of the most critical life stages. The diets considered in this study are single strains diet of Cylindrotheca fusiformis CCAP 1017/2 – CYL, Isochrysis galbana CCAP 927/1– ISO, Monodopsis subterranean CCAP 848/1 – MONO, Nannochloropsis oceanica CCAP 849/10– NANNO and a commercial algae paste –SP.The diets had a significant effect on spat GR and WI, and according to their efficacy resulted ranked as follows: ISO>NANNO/CYL>SP>MONO. Spat FA composition and neutral lipid content (principally triacylglycerols - TG), were influenced by the diets. Furthermore, untargeted lipidomics also showed shifts in several phospholipid species, with changes related to the essential PUFA available from the diet. TG content, neutral lipids and several TG and FA species were correlated (Spearman R2>0.8 FDR p<0.05) with spat WI, suggesting their possible application as markers of mussel juvenile condition. The availability of dietary essential PUFA deeply modified the spat lipidome both for neutral and for polar lipids. This change in the lipidome could have major impacts on their ecology and their production for food.

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Comprehensive lipid profiling in the Mediterranean mussel (Mytilus galloprovincialis) using hyphenated and multidimensional chromatography techniques coupled to mass spectrometry detection

Cited by 36 publications

References 43 publications

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulis L. 1758), a key economic and ecological species

Large-scale lipid analysis with C=C location and sn-position isomer resolving power

Lipidomics analysis of juveniles’ blue mussels (Mytilus edulisL. 1758), a key economic and ecological species

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