Comprehensive in vitro polymer type, concentration, and size correlation analysis to microplastic toxicity and inflammation

Brito, Walison Augusto da Silva; Singer, Debora; Miebach, Lea; Saadati, Fariba; Wende, Kristian; Schmidt, Anke; Bekeschus, Sander

doi:10.1016/j.scitotenv.2022.158731

Cited by 28 publications

(24 citation statements)

References 62 publications

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“…Cytoplasmic and intracellular membrane structures were stained with a blue fluorescent dye (CellBriteBlue; Biotium, San Fransisco, USA) and visualized along with fluorescent NMP uptake into the skin cells using a confocal laser scanning high-content imaging system and several z-stack image series integrated into maximum intensity projections for improved visibility (Operetta CLS; PerkinElmer, Germany). Quantification, however, was based on true 3D object quantification, a recent feature added to Harmony 4.9 software (PerkinElmer, Germany) package, as we had described recently [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

“…Environmentally relevant concentrations of NMP are between 1.6 µg/mL, found in human blood samples [29], and 100 to 1000 µg/mL to facilitate studies on biological effects [30]. In line with the literature and own experimental findings [31], 10 × concentrated NMP suspensions were added, reaching 1 × final concentration of 100 µg/mL in PBS. Skin cell monolayers (70% confluence) were incubated with NMP in corresponding cell culture dishes ranging from 6 cm 2 -dishes for generating RNA and protein lysates (5 × 10 5 cells) over 12-well plates (1.5 × 10 5 cells) for immunofluorescence-imaging to 96-well plates (5 × 10 3 cells) for metabolic activity and viability measurements.…”

Section: Polymer Particle Design and Exposure To Biological Materialsmentioning

confidence: 99%

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Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

et al. 2023

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Nano- and microplastic particles (NMP) are strong environmental contaminants affecting marine ecosystems and human health. The negligible use of biodegradable plastics and the lack of knowledge about plastic uptake, accumulation, and functional consequences led us to investigate the short- and long-term effects in freshly isolated skin cells from mice. Using fluorescent NMP of several sizes (200 nm to 6 µm), efficient cellular uptake was observed, causing, however, only minor acute toxicity as metabolic activity and apoptosis data suggested, albeit changes in intracellular reactive species and thiol levels were observed. The internalized NMP induced an altered expression of various targets of the nuclear factor-2-related transcription factor 2 pathway and were accompanied by changed antioxidant and oxidative stress signaling responses, as suggested by altered heme oxygenase 1 and glutathione peroxide 2 levels. A highly increased beta-catenin expression under acute but not chronic NMP exposure was concomitant with a strong translocation from membrane to the nucleus and subsequent transcription activation of Wnt signaling target genes after both single-dose and chronic long-term NMP exposure. Moreover, fibroblast-to-myofibroblast transdifferentiation accompanied by an increase of α smooth muscle actin and collagen expression was observed. Together with several NMP-induced changes in junctional and adherence protein expression, our study for the first time elucidates the acute and chronic effects of NMP of different sizes in primary skin cells' signaling and functional biology, contributing to a better understanding of nano- and microplastic to health risks in higher vertebrates.

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Section: Methodsmentioning

confidence: 99%

Section: Polymer Particle Design and Exposure To Biological Materialsmentioning

confidence: 99%

Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

et al. 2023

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“…In contrast, the 1 μm PS-NH 2 did not show a concentration-depending effect on any of the investigated cell lines, even at longer exposure times of 72 h. The 50 nm PS-NH 2 particles are described to induce early apoptosis, while cellular systems seem less affected by larger-sized PS-NH 2 . 60,61 Due to the particle size and the charge distribution on the surface of the particles, endocytic processes are more likely for smaller particles than for larger ones. The small PS-NH 2 are reported to induce oxidative stress in the cells during acute MNPP exposure.…”

Section: Protein Corona Around Mnpps Alters Metabolism Of Hacatmentioning

confidence: 99%

Nanoplastic Size and Surface Chemistry Dictate Decoration by Human Saliva Proteins

Dorsch,

Förschner,

Ravandeh

et al. 2024

ACS Appl. Mater. Interfaces

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Environmental pollution with plastic polymers has become a global problem, leaving no continent and habitat unaffected. Plastic waste is broken down into smaller parts by environmental factors, which generate micro- and nanoplastic particles (MNPPs), ultimately ending up in the human food chain. Before entering the human body, MNPPs make their first contact with saliva in the human mouth. However, it is unknown what proteins attach to plastic particles and whether such protein corona formation is affected by the particle’s biophysical properties. To this end, we employed polystyrene MNPPs of two different sizes and three different charges and incubated them individually with saliva donated by healthy human volunteers. Particle zeta potential and size analyses were performed using dynamic light scattering complemented by nanoliquid chromatography high-resolution mass spectrometry (nLC/HRMS) to qualitatively and quantitatively reveal the protein soft and hard corona for each particle type. Notably, protein profiles and relative quantities were dictated by plastic particle size and charge, which in turn affected their hydrodynamic size, polydispersity, and zeta potential. Strikingly, we provide evidence of the latter to be dynamic processes depending on exposure times. Smaller particles seemed to be more reactive with the surrounding proteins, and cultures of the particles with five different cell lines (HeLa, HEK293, A549, HepG2, and HaCaT) indicated protein corona effects on cellular metabolic activity and genotoxicity. In summary, our data suggest nanoplastic size and surface chemistry dictate the decoration by human saliva proteins, with important implications for MNPP uptake in humans.

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“…17 The cellular internalization of MNPs can have various biological effects that impact normal functioning of the body, including oxidative stress, inflammatory responses, metabolic changes, and toxic effects. 18,19 One of the first cells that respond when MNPs enter the body are macrophages, which are important components of the immune system and found in almost all tissues of the body. 20 Macrophages are derived from monocytes that migrate from the bloodstream to the site of infection or inflammation 21 and orchestrate a rapid immune response.…”

Section: Introductionmentioning

confidence: 99%

“…MNPs have been detected in urine, breast milk, embryo, and, for the first time recently in 2022, in human blood at an average concentration of 1.6 μg/mL . The cellular internalization of MNPs can have various biological effects that impact normal functioning of the body, including oxidative stress, inflammatory responses, metabolic changes, and toxic effects. , …”

Section: Introductionmentioning

confidence: 99%

Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells

Liu,

Wang,

Sheng

et al. 2024

Environ. Sci. Technol.

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Micro(nano)plastics (MNPs) are emerging pollutants that can adsorb pollutants in the environment and biological molecules and ultimately affect human health. However, the aspects of adsorption of intracellular proteins onto MNPs and its biological effects in cells have not been investigated to date. The present study revealed that 100 nm polystyrene nanoplastics (NPs) could be internalized by THP-1 cells and specifically adsorbed intracellular proteins. In total, 773 proteins adsorbed onto NPs with high reliability were identified using the proteomics approach and analyzed via bioinformatics to predict the route and distribution of NPs following cellular internalization. The representative proteins identified via the Kyoto Encyclopedia of Genes and Genomes pathway analysis were further investigated to characterize protein adsorption onto NPs and its biological effects. The analysis revealed that NPs affect glycolysis through pyruvate kinase M (PKM) adsorption, trigger the unfolded protein response through the adsorption of ribophorin 1 (RPN1) and heat shock 70 protein 8 (HSPA8), and are chiefly internalized into cells through clathrin-mediated endocytosis with concomitant clathrin heavy chain (CLTC) adsorption. Therefore, this work provides new insights and research strategies for the study of the biological effects caused by NPs.

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Comprehensive in vitro polymer type, concentration, and size correlation analysis to microplastic toxicity and inflammation

Cited by 28 publications

References 62 publications

Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

Short- and long-term polystyrene nano- and microplastic exposure promotes oxidative stress and divergently affects skin cell architecture and Wnt/beta-catenin signaling

Nanoplastic Size and Surface Chemistry Dictate Decoration by Human Saliva Proteins

Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells

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