Comprehensive identification of 125 multifarious constituents in Shuang–huang–lian powder injection by HPLC-DAD-ESI-IT-TOF-MS

Sun, Hongyang; Liu, Meixian; Lin, Zongtao; Jiang, Haixiu; Niu, Yanyan; Wang, Hong; Chen, Shizhong

doi:10.1016/j.jpba.2015.06.013

Cited by 59 publications

(54 citation statements)

References 22 publications

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“…The ions at m/z 179 and 191 in negative mode were deprotonated ions of caffeic acid ([CA-H] − ) and quinic acid ([QA-H] − ). Compared with standard compounds and previous reports [25, 28–32], peaks 3 , 4 , and 5 were thus identified as isomers of dicaffeoylquinic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C, respectively.…”

Section: Resultsmentioning

confidence: 51%

Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots ofIlex pubescensHook. et Arn. Using a Combination of Ultrafiltration and LC–MS

Liu

Lin

Chen

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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The cyclic nucleotide phosphodiesterase (PDE) plays an important role in regulating the levels of second messenger molecules cAMP and cGMP. Various PDE inhibitors have been successfully developed into drugs for targeted diseases. In addition, PDE inhibitors can also be found in different foods and natural medicines. In this study, ultrafiltration liquid chromatography–diode-array detector–electrospray ionization–ion-trap–time-of-flight–mass spectrometry (ultrafiltration LC–DAD–ESI–IT–TOF–MS) was applied to screen PDE inhibitors from the roots of Ilex pubescens Hook. et Arn. As a result, 11 major compounds were identified in I. pubescens roots, with nine compounds as potential PDE inhibitors, among which five were further confirmed to be active against PDEI and PDE5A dose-dependently in vitro, with ilexsaponin A1 and ilexsaponin B2 being the strongest. HPLC quantification of these bioactive compounds suggested that they are major components in the plant. The results demonstrate that ultrafiltration LC–DAD–ESI–IT–TOF–MS is an efficient method for rapid screening of PDE inhibitors from natural medicines.

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Section: Resultsmentioning

confidence: 51%

Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots ofIlex pubescensHook. et Arn. Using a Combination of Ultrafiltration and LC–MS

Liu

Lin

Chen

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…The MS 2 spectrum of m/z 191 presented the fragments of m/z 127, 85 and 59, the base peak at m/z 127 corresponding to the loss of [M − H − H 2 O − HCOOH] − . Hence, it was identified as quinic acid by comparison with the published data (de la Luz Cádiz‐Gurrea et al, ; Santos et al, ; Sun et al, ).…”

Section: Resultsmentioning

confidence: 85%

“…Peaks 1 – 3 , 5 , 32 , 33 and 37 were identified as quinic acid, citric acid, succinic acid, vanillic acid, carnosic acid, linolenic acid and palmitic acid, respectively, by comparing their MS/MS profiles with the published data (de la Luz Cádiz‐Gurrea, Fernández‐Arroyo, Joven, & Segura‐Carretero, ; Maringer, Ibáñez, Buchberger, Klampfl, & Causon, ; Riffault et al, ; Santos, Freire, Domingues, Silvestre, & Pascoal, ; Sun et al, ; Zeng, Xiao, Liu, & Liang, ). For these peaks with one or two carboxyl groups, the characteristic fragments were supported by their common losses of CO 2 (44 Da).…”

Section: Resultsmentioning

confidence: 99%

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Chemical identification and quality evaluation of Lycopus lucidus Turcz by UHPLC‐Q‐TOF‐MS and HPLC‐MS/MS and hierarchical clustering analysis

Ren

Ding

Sun

et al. 2016

Biomedical Chromatography

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Lycopus lucidus Turcz has been used as a traditional phytomedicine for menstrual disorder, amenorrhea, menstrual cramps, inflammation and cardiovascular diseases. However, there is not enough information about identification and quantification for the chemical constituents of L. lucidus Turcz. In this work, a simple, rapid and sensitive UHPLC-Q-TOF-MS method was developed for characterization and identification of the phytochemical compositions in L. lucidus Turcz in negative ion mode. A total of 37 compounds, including 15 phenolic acids, 12 flavonoids, three triterpenoids and seven organic acids were tentatively characterized and identified by means of the retention time, accurate mass and characteristic fragment ions. Thirteen compounds were reported for the first time in L. lucidus Turcz. Among of them, 11 compounds were further quantified by multiple reactions monitoring. The results showed good performance with respect to linearity (r > 0.9959), repeatability (RSD < 2.6%), intra- and inter-day precision (RSD < 3.2%), recovery (93.1-104.9%), and lower limit of quantification (5-50 ng/mL). Subsequently, the results were analyzed and classified by hierarchical cluster analysis. The research could be applied for identification and quality evaluation for L. lucidus Turcz.

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“…11 compounds were unambiguously identified by comparing with reference standards. For other compounds, the structures were tentatively identified by comparing with previous studies , according to accurate mass, MS/MS data, fragmentation rules and chromatographic behavior. However, the trace peaks in the single herbs might be missed in the TCM formula.…”

Section: Resultsmentioning

confidence: 99%

Identification of chemical constituents in traditional Chinese medicine formula using HPLC coupled with linear ion trap-Orbitrap MS from high doses of medicinal materials to equivalent doses of formula: Study on Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules

et al. 2016

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High-resolution mass spectrometry has been a powerful tool for the research of chemical constituents in traditional Chinese medicine (TCM) formulas. However, the chromatographic peaks were difficult to discriminate clearly in data collection or analysis because of the complexity and the greatly different content of the constituents in TCM formula, which increased the difficulty of identification. In this study, a high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry based strategy focused on the comprehensive identification of TCM formula constituents was developed. Identification was carried out from a high dose of medicinal materials to equivalent dose of formula. Meanwhile, combined with mass spectrometry data, chromatographic behaviors, reference standards and previous reports, the identification of constituents in Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules was described. 169 compounds were unambiguously or tentatively characterized, mainly including flavonoids, alkaloids, triterpenic acids, triterpene saponins, lactones, sesquiterpenoids and some other compounds. Among them, 11 compounds were unambiguously confirmed by comparing with reference standards. These results demonstrated that the method was effective and reliable for comprehensive identification of constituents of Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules extracts and reveal the material basis of its therapeutic effects. This strategy might propose a research idea for the characterization of multi-constituents in TCM formula.

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Comprehensive identification of 125 multifarious constituents in Shuang–huang–lian powder injection by HPLC-DAD-ESI-IT-TOF-MS

Cited by 59 publications

References 22 publications

Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots ofIlex pubescensHook. et Arn. Using a Combination of Ultrafiltration and LC–MS

Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots ofIlex pubescensHook. et Arn. Using a Combination of Ultrafiltration and LC–MS

Chemical identification and quality evaluation of Lycopus lucidus Turcz by UHPLC‐Q‐TOF‐MS and HPLC‐MS/MS and hierarchical clustering analysis

Identification of chemical constituents in traditional Chinese medicine formula using HPLC coupled with linear ion trap-Orbitrap MS from high doses of medicinal materials to equivalent doses of formula: Study on Xiang-Sha-Liu-Jun-Zi-Jia-Jian granules

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