Comprehensive HPLC fingerprint analysis based on a two-step extraction method for quality evaluation of Perilla frutescens (L.) Britt

Abstract

Abundant chemical components are the key to ensure the evaluation accuracy of the fingerprint analysis of traditional Chinese medicines (TCMs). A two-step extraction method combining supercritical fluid extraction (SFE) and...

Lycopodium Alkaloids from Huperzia goebelii

“…Among them, compounds 2, 3, 5, and 6 were isolated from H. goebelii for the first time. HPLC fingerprint analysis, a comprehensive quality evaluation tool based on chemical composition of traditional medicine, [16] was employed to assess whether huperzine A has a high content in the alkaloidal extract of H. goebelii. The analysis was performed with MeCNÀ H 2 OÀ NH 4 OH (0 min, 10 : 90:0.05; 40 min, 100 : 0 : 0.05; 50 min, 100 : 0 : 0.05; v/v/v, 0.8 mL/min), and the result indicated a high content of huperzine A (t R = 13.543 min) in H. goebelii alkaloidal extract (Figure 4).…”

Phytochemical Profile of Alkaloid Extract from Dendrobium huoshanense and Inhibitory Effects against Oxidative Stress in H2O2‐Induced PC12 Cells

“…HPLC fingerprint analysis is a comprehensive quality evaluation tool based on chemical composition of TCM, [33] for example the feature of D. officinale from different areas. [34] To further discover the alkaloid composition, a HPLC method with great separation efficiency and reproducibility was firstly established for fingerprint assessment of DHAE in three different extraction solvents, and the corresponding chromatograms were shown in Figure 1.…”