Comprehensive genomic analysis and expression profiling of diacylglycerol kinase gene family in Malus prunifolia (Willd.) Borkh

Li, Yali; Tan, Yanxiao; Shao, Yun; Li, Mingjun; Ma, Fengwang

doi:10.1016/j.gene.2015.02.029

Cited by 26 publications

(48 citation statements)

References 38 publications

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“…DGKs are widely distributed in eukaryotes. In silico identi cation has been used for the functional prediction of DGKs family members in Arabidopsis, rice, maize, apple, and soybean [5,[9][10][11]13]. Research assessing the response of DGKs to osmotic stress has mainly been performed through analysis of transcriptome and examination of mutants.…”

Section: Discussionmentioning

confidence: 99%

“…As in other studied plants, TaDGKs have the classical generalized structure as seen in other studied plants ( Fig.5). Additionally, the two C1 domains harbor the sequences HX 14 [11,13,14]. By sequence alignment, we found the upstream basic region and extCRD-like domain were substantially conserved, with only slight variation: in the basic region, conserved KA residues were replaced by KVin TaDGK1 (A/B/D), and the anking residue V of extCRD-like was replaced by L in TaDGK4A (Fig.…”

Section: Protein Domains and Sequence Characterization Of Tadgk Genesmentioning

confidence: 91%

“…We isolated 20 TaDGKs in hexaploid wheat (T. aestivum) using a genome-wide approach. The number of DGKs in wheat is approximately three-fold higher than that in Arabidopsis (i.e., seven DGKs) [7], rice (eight DGKs) [10], and other plant species [9,11,13]. This is because the ancestor of allohexaploid bread wheat (T. aestivum) underwent three polyploidizations [34].…”

Section: Expansion Of the Dgk Gene Family In Hexaploid Wheatmentioning

confidence: 99%

“…Plant DGKs have been cloned from many plant species, including Arabidopsis thaliana [6][7], tomato [8], maize (Zea mays) [9], rice (Oryza sativa) [10], Malus prunifolia [11], tobacco [12], and soybean (Glycine max) [13]. Based on their gene architectures, evolutionary relationships, and sequences, plants DGKs have been classi ed into three distinct phylogenetic clusters [5].…”

Section: Introductionmentioning

confidence: 99%

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Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat

Jia

et al. 2020

Preprint

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Background The inositol phospholipid signaling system, which is based on the metabolism of phosphoinositide (PI), mediates plant growth, development, and responses to adversity. Diacylglycerol kinase (DGK) is one of the key enzymes in the PI-cycle, which catalyzes the phosphorylation of diacylglycerol (DAG) to form phosphatidic acid (PA). To date, comprehensive genomic and functional analyses of DGK genes have not been reported in wheat. Results In this study, 20 DGK gene family members from the heterologous hexaploid wheat genome (TaDGKs) were identified and analyzed. Each putative protein was found to consist of a DGK catalytic domain and a accessory domain. The analyses of phylogenetic and gene structure revealed that each TaDGK gene could be grouped to clusters I, II, or III. In each phylogenetic subgroup, the TaDGKs demonstrated high conservation in functional domains, for example gene structure and amino acid sequences. By cloning, four coding sequences were ascertained from Chinese spring wheat. Expression analysis of these four genes revealed that each had a unique spatial and developmental expression pattern, indicating their functional diversification in wheat growth and development processes. Additionally, TaDGKs were also prominently up-regulated express under salt and drought stresses, suggesting their possible roles in dealing with adversity environment. Further cis-regulatory elements analysis elucidated transcriptional regulation and potential biological functions. Conclusions These results provide valuable information for understanding the putative functions of DGK genes in wheat, and conduce to ulterior functional analysis of this pivotal gene family. The 20 TaDGKs identified and analyzed in this study provide a strong foundation for further exploration of the biological function and regulatory mechanisms of TaDGKs in response to environmental stimuli.

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Section: Discussionmentioning

confidence: 99%

Section: Protein Domains and Sequence Characterization Of Tadgk Genesmentioning

confidence: 91%

Section: Expansion Of the Dgk Gene Family In Hexaploid Wheatmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat

Jia

et al. 2020

Preprint

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“…OsOXO4 was reported to contribute to apoplastic ROS accumulation under drought conditions (Voothuluru and Sharp, 2013;. OsDGK8 plays a role in generating phosphatidic acid (PA) in response to biotic and abiotic stresses (Li et al, 2015), suggesting that it may participate in PA-dependent ROS production. OsCBSX10, UDP-glycosyltransferase, Yr10-like protein, OsRING-1 and their Arabidopsis homologs were documented to perform functions in mitochondrial ROS accumulation, pathogen-induced oxidative burst as well as hypersensitive responses (Meng et al, 2006;Coram et al, 2010;Shin et al, 2020).…”

Section: Transcriptome Analyses Of Drought-stressed Idr1-1 Mutants Rementioning

confidence: 99%

Mutation of IDR1 enhances drought tolerance by reducing ROS production and activating ROS scavenging in rice

Wang

et al. 2020

Preprint

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To discover new mutant alleles conferring enhanced tolerance to drought stress, we screened a mutagenized rice population (cv. IAPAR9) and identified a mutant, named idr1-1 (for increased drought resistance 1-1), with obviously increased drought tolerance under upland field conditions. The idr1-1 mutant possessed a significantly enhanced ability to tolerate high-drought stress in different trials. Map-based cloning revealed that the gene LOC_Os05g26890 (corresponding to D1 or RGA1 gene), residing in the mapping region of IDR1 locus, carried a single-base deletion in the idr1-1 mutant, which caused a frameshift and premature translation termination. Complementation tests indicated that such a mutation was indeed responsible for the elevated drought tolerance in idr1-1 mutant. IDR1 protein was localized in nucleus and to plasma membrane or cell periphery. Further investigations indicated that the significantly increased drought tolerance in idr1-1 mutant stemmed from a range of physiological and morphological changes occurring in such a mutant, including greater leaf potentials, increased proline contents, heightened leaf thickness, and upregulation of antioxidant-synthesizing and drought-induced genes, etc., under drought-stressed conditions. Especially, ROS production from NADPH oxidases and chloroplasts might be remarkably impaired, while ROS-scavenging ability appeared to be markedly enhanced as a result of significantly elevated expression of a dozen ROS-scavenging enzyme genes in idr1-1 mutant under drought-stressed conditions. Besides, IDR1 physically interacted with TUD1, and idr1-1 mutant showed impaired EBR responsiveness. Altogether, these results suggest that mutation of IDR1 leads to alterations of multiple layers of regulations, which ultimately confers obviously enhanced drought tolerance to the idr1-1 mutant.One-sentence summaryMutation of IDR1 significantly enhances drought tolerance in an upland cultivar IAPAR9 by decreasing apoplastic and chloroplastic ROS production and increasing ROS-scavenging ability

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Genome-Wide Identification and Expression Analysis of DGK (Diacylglycerol Kinase) Genes in Common Bean

Yeken

Özer

Çi̇ftçi̇

2022

J Plant Growth Regul

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Comprehensive genomic analysis and expression profiling of diacylglycerol kinase gene family in Malus prunifolia (Willd.) Borkh

Cited by 26 publications

References 38 publications

Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat

Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat

Mutation of IDR1 enhances drought tolerance by reducing ROS production and activating ROS scavenging in rice

Genome-Wide Identification and Expression Analysis of DGK (Diacylglycerol Kinase) Genes in Common Bean

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