Comprehensive Genome-Wide Analysis of the Catalase Enzyme Toolbox in Potato (Solanum tuberosum L.)

Abstract

As antioxidant enzymes, catalase (CAT) protects organisms from oxidative stress via the production of reactive oxygen species (ROS). These enzymes play important roles in diverse biological processes. However, little is known about the CAT genes in potato plants despite its important economical rank of this crop in the world. Yet, abiotic and biotic stresses severely hinder growth and development of the plants which affects the production and quality of the crop. To de ne the possible roles of CAT genes under various stresses, a genome-wide analysis of CAT gene family has been performed in potato plant.In this study, the StCAT gene's structure, secondary and 3D protein structure, physicochemical properties, synteny analysis, phylogenetic tree and also expression pro ling under various developmental and environmental cues were predicted using bioinformatics tools. The expression analysis by RT-PCR was performed using commercial potato cultivar.Three genes encoding StCAT that code for three proteins each of size 492 aa, interrupted by seven introns have been identi ed in potatoes. StCAT proteins were found to be localized in the peroxisome which is judged as the main H 2 O 2 cell production site during different processes. Many regulating cis-elements related to stress responses and plant hormones signaling were found in the promoter sequence of each gene. The analysis of motifs and phylogenetic trees showed that StCAT are closer to their homologous in S. lycopersicum and share a 41% -95% identity with other plants' CATs. Expression pro ling revealed that StCAT1 is the constitutively expressive member; while StCAT2 and StCAT3 are the stress-responsive members. Key MessageThe genome-wide analysis of the catalase genes in S. tuberosum provides new information that can help characterize the molecular mechanisms, and that StCAT1 would be an interesting candidate for potato breeding technologies.

Heat responsive gene StGATA2 functions in plant growth, photosynthesis and antioxidant defense under heat stress conditions

“…Catalase functions as antioxidant enzyme, dissolving hydrogen peroxide into water and oxygen peroxisomes, of which encoding genes were found with cis-elements related to stress responses and plant hormones signaling in potato plants ( Jbir Koubaa et al., 2023 ). Superoxide dismutase encoded by StCuZnSODs , StFeSOD3 and StMnSOD play a significant role in catalyzing the conversion or dismutation of toxic superoxide anion radical into H 2 O 2 and O 2 , representing the first line of antioxidant defense against ROS induced by heat stress ( Rudić et al., 2022 ).…”