Comprehensive Genome-Wide Analysis of Histone Acetylation Genes in Roses and Expression Analyses in Response to Heat Stress

Abstract

Roses have high economic values as garden plants and for cut-flower and cosmetics industries. The growth and development of rose plants is affected by exposure to high temperature. Histone acetylation plays an important role in plant development and responses to various stresses. It is a dynamic and reversible process mediated by histone deacetylases (HDAC) and histone acetyltransferases (HAT). However, information on HDAC and HAT genes of roses is scarce. Here, 23 HDAC genes and 10 HAT genes were identified in the Rosa chinensis ‘Old Blush’ genome. Their gene structures, conserved motifs, physicochemical properties, phylogeny, and synteny were assessed. Analyses of the expression of HDAC and HAT genes using available RNAseq data showed that these genes exhibit different expression patterns in different organs of the three analyzed rose cultivars. After heat stress, while the expression of most HDAC genes tend to be down-regulated, that of HAT genes was up-regulated when rose plants were grown at high-temperature conditions. These data suggest that rose likely respond to high-temperature exposure via modification in histone acetylation, and, thus, paves the way to more studies in order to elucidate in roses the molecular mechanisms underlying rose plants development and flowering.

Plant responses to abiotic stress regulated by histone acetylation

“…Plants have evolved mechanisms to adapt and attenuate the hazards of temperature stress, with HAT/HDACs playing a significant role. Heat stress increases the expression of HAT genes in rose, suggesting histone acetylation adjustments in response ( Wu et al., 2022 ). GCN5 regulated heat stress responsive genes HSFA3 and UVH6 by facilitating H3K9ac and H3K14ac to maintain thermotolerance in Arabidopsis ( Hu et al., 2015 ).…”