Comprehensive genome analyses ofSellimonas intestinalis, a potential biomarker of homeostasis gut recovery

Abstract

Comprehensive genome analyses of Sellimonas intestinalis, a potential 19 biomarker of homeostasis gut recovery 20 Sellimonas intestinalis is a Gram positive and anaerobic bacterial species 21 previously considered as uncultivable. Although little is known about this 22 Lachnospiraceae family member, its increased abundance has been reported in 23 patients who recovered intestinal homeostasis after dysbiosis events. In this 24 context, the aim of this work was taken advantage of a culturomics protocol that 25 allowed the recovery species extremely oxygen-sensitive from faecal samples, 26 which led to the establishment of an S. intestinalis isolate. Whole genome 27 sequencing and taxonomic allocation confirmation were the base to develop 28 comparative analyses including 11 public genomes closely related. 29 Phylogeographic analysis revealed the existence of three lineages (linage-I 30 including isolates from Chile and France, linage-II from South Korea and Finland, 31 and linage-III from China and one isolate from USA). Pangenome analysis on the 32 established dataset revealed that although S. intestinalis seems to have a highly 33 conserved genome (with 50.1% of its coding potential being part of the 34 coregenome), some recombination signals were evidenced. The identification of 35 cluster of orthologous groups revealed a high number of genes involved in 36 metabolism, including amino acid and carbohydrate transport as well as energy 37 production and conversion, which matches with the metabolic profile previously 38 reported for healthy microbiota. Additionally, virulence factors and antimicrobial 39 resistance genes were found (mainly in linage-III), which could favour their 40 survival during antibiotic-induced dysbiosis. These findings provide the basis of 41 knowledge about this species with potential as a bioindicator of intestinal 42 homeostasis recovery and contribute to advance in the characterization of gut 43 microbiota members with beneficial potential.44

Examining the effects of Salmonella phage on the caecal microbiota and metabolome features in Salmonella-free broilers

“…However, higher abundance of this genus may not be of interest, as it could cause diseases in broilers and has been negatively correlated with body weight ( Thibodeau et al, 2015 ; Lundberg et al, 2021 ). Conversely, a higher abundance of Sellimonas has been reported to be involved in recovered intestinal homeostasis after dysbiosis events ( Muñoz et al, 2020 ). The abundance rates of Lactobacillus , Anaeroplasma and Clostridia_vadinBB60_group were higher when phages were included in the feed.…”

Exploration of the Characteristics of Intestinal Microbiota and Metabolomics in Different Rat Models of Mongolian Medicine

“…There were 11, 18, and 8 bacterial biomarkers that were increased only in Heyi, Xila, and Badagan rats, respectively. Some of these bacterial genera have been associated with diseases, such as Faecalibacterium [ 34 ], Prevotella_1 [ 35 ], Prevotellaceae [ 36 ], Alistipes [ 37 ], Bilophila [ 38 ], Hungatella [ 39 ], and Sellimonas [ 40 ], which might contribute to diseases in Mongolian medicine. In addition, the differential KEGG pathways were found between Mongolian medicine rat models and control group rats.…”