Comprehensive evaluation of two Astragalus species (A. campylosema and A. hirsutus) based on biological, toxicological properties and chemical profiling

Kurt-Celep, İnci; Zengin, Gökhan; Sinan, Kouadio Ibrahime; Ak, Güneş; Elbasan, Fevzi; Yıldıztugay, Evren; Maggi, Filippo; Caprioli, Giovanni; Angeloni, Simone; Sharmeen, Jugreet B.; Mahomoodally, Mohamad Fawzi

doi:10.1016/j.fct.2021.112330

Cited by 13 publications

(24 citation statements)

References 47 publications

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“…In the formula of Modified Buzhong Yiqi Decoction, Astragalus enters the spleen and lung meridians, can invigorate the middle and benefit qi, raise yang and ascend sag, and can solidify the surface and stop sweating, so it is reused as the king ( 11 ). Ginseng is great for nourishing vitality, fried Atractylodes nourishes Qi and spleen, Licorice nourishes the middle and invigorating Qi, and helps Astragalus to nourish Qi and strengthen the spleen, all of which are ministerial medicines ( 12 , 13 ) and the patient's qi deficiency for a long time often damages the blood.…”

Section: Discussionmentioning

confidence: 99%

Clinical Study on the Treatment of Female Stress Urinary Incontinence With Modified Buzhong Yiqi Decoction

et al. 2022

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PurposeTo study the clinical application value of Modified Buzhong Yiqi Decoction in the treatment of female stress urinary incontinence (SUI).MethodsA total of 103 female patients with SUI were included in this study, 13 were lost to follow-up, and the final number of studies was 90. General information about the patients, including age, years of menopause, body mass index (BMI), reproductive history, chronic respiratory disease, hypertension, and diabetes, were recorded. All the patients were treated with Modified Buzhong Yiqi Decoction alone for 4 weeks. The Patient Global Impression of Improvement (PGI-I), the International Consultation on Incontinence Questionnaire-Urinary Incontinence Short Form (ICI-QSF) and 72-h voiding diary were used to evaluate the patients' subjective symptoms and urinary incontinence degree before treatment, 1 month after treatment and 1 year after treatment, the efficacy and efficacy-related factors of Modified Buzhong Yiqi Decoction in the treatment of female SUI were analyzed.ResultsOne month after Modified Buzhong Yiqi Decoction treatment, compared with before treatment, the PGI-I questionnaire was very much better (68.89%), much better (8.89%), a little better (12.33%), no change (8.89%), the ICI-QSF score decreased (P < 0.05), and 72-h urine leakage frequency decreased (P < 0.05); One year after treatment compared with before treatment, the PGI-I questionnaire was very much better (40.00%), much better (17.78%), a little better (12.22%), no change (30.00%), the ICI-QSF score decreased (P < 0.05), and 72-h urine leakage frequency decreased (P < 0.05); and 1 year after treatment compared with 1 month after treatment, the ratio of very much better at 1 year after treatment was significantly decreased (P < 0.05), the score of the ICI-QSF was significantly increased (P < 0.05), and 72-h urine leakage frequency was significantly increased (P < 0.05). The correlation analysis showed that the efficacy at 1 month after treatment was negatively correlated with the severity of SUI and chronic respiratory diseases, but was not significantly correlated with age, menopause status, BMI, number of pregnancies, and number of births. The efficacy at 1 year after treatment was negatively correlated with the severity of SUI, chronic respiratory disease, age, and number of births and was positively correlated with BMI, but not significantly correlated with menopause status and number of pregnancies.ConclusionModified Buzhong Yiqi Decoction can effectively treat SUI in women. The efficacy is related to the severity of SUI and chronic abdominal hypertension, but the long-term efficacy decreases.

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Section: Discussionmentioning

confidence: 99%

Clinical Study on the Treatment of Female Stress Urinary Incontinence With Modified Buzhong Yiqi Decoction

et al. 2022

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“…The medium was also supplemented with 10% fetal bovine serum (FBS), 1% Penicillin (10 U/mL)/Streptomycin (10 mg/mL), 4.5 g/L of L-glutamine, and 3.7 g/L of sodium pyruvate [ 31 , 32 ]. The HT-29 cells were incubated at 37 °C in humidified 5% CO 2 , which is the optimal growth condition for mammalian cells [ 33 ]. When the cells were 90% confluent in the growth dishes, they were passaged by removing them from the culture vessel with 0.05% trypsin/EDTA, and they were stocked in the liquid nitrogen tank for further experiments.…”

Section: Methodsmentioning

confidence: 99%

An In-Depth Study on the Metabolite Profile and Biological Properties of Primula auriculata Extracts: A Fascinating Sparkle on the Way from Nature to Functional Applications

et al. 2022

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The biological activity of the aerial part and rhizomes of Primula auriculata were assessed for the first time. The biological activities (antioxidant properties, enzyme inhibition, and AGE inhibition) as well as the phenolic and flavonoid contents of the ethyl acetate, ethanol, hydro-ethanol and water extracts of P. auriculata aerial parts and rhizomes were determined. Cell viability assays and gelatin zymography were also performed for MMP-2/-9 to determine the molecular mechanisms of action. The gene expression for MMPs was described with RT-PCR. The levels of various proteins, including phospho-Nf-κB, BCL-2, BAX, p-53, and cyclin D1 as well as RAGE were measured using Western blot analysis. The hydro-ethanol extract of the aerial part possessed the highest phenolic (56.81 mg GAE/g) and flavonoid (63.92 mg RE/g) contents. In-depth profiling of the specialized metabolites by ultra-high-performance liquid chromatography—high-resolution mass spectrometry (UHPLC-HRMS) allowed for the identification and annotation of 65 compounds, including phenolic acids and glycosides, flavones, flavonols, chalcones, dihydrochalcones, and saponins. The hydro-ethanol extract of the aerial parts (132.65, 180.87, 172.46, and 108.37 mg TE/g, for the DPPH, ABTS, CUPRAC, and FRAP assays, respectively) and the ethanol extract of the rhizomes (415.06, 638.30, 477.77, and 301.02 mg TE/g, for the DPPH, ABTS, CUPRAC, and FRAP assays, respectively) exhibited the highest free radical scavenging and reducing activities. The ethanol and hydro-ethanol extracts of both the P. auriculata aerial part and rhizomes exhibited higher inhibitory activity against acetylcholinesterase, while the hydro-ethanol extracts (1.16 mmol ACAE/g, for both the aerial part and rhizomes extracts) were more active in the inhibition of α-glucosidase. After the treatment of an HT-29 colorectal cancer cell line with the extracts, the apoptosis mechanism was initiated, the integrity of the ECM was remodeled, and cell proliferation was also taken under control. In this way, Primula extracts were shown to be potential drug sources in the treatment of colorectal cancer. They were also detected as natural MMP inhibitors. The findings presented in the present study appraise the bioactivity of P. auriculata, an understudied species. Additional assessment is required to evaluate the cytotoxicity of P. auriculata as well as its activity in ex vivo systems.

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Section: Methodsmentioning

confidence: 99%

“…The H. humile extracts were analyzed for the 25 biologically active compounds such as phenolic acids, iridoids, chlorogenic acids, flavonoids and xanthones by following a developed procedure before but with some modifications. [73] In summary, the dried extracts were dissolved in methanol (5 mg/mL) helping with 10 min of sonication and before high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS) analysis, a 0.2 μm filter was used for sample filtering. The system used for the present research was an Agilent 1290 Infinity series and a Triple Quadrupole 6420 from Agilent Technology (Santa Clara, CA) with an electrospray ionization (ESI) source working in negative ionization mode.…”

Section: Bioactive Compound Quantitation By Hplc/ms/ms Systemmentioning

confidence: 99%

Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound‐Assisted Extraction Method

Öçal

Altunoğlu

Angeloni

et al. 2022

Chemistry & Biodiversity

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As the safety and effectiveness of synthetic drugs remain in doubt, researchers are trying to develop natural medicines from medicinal plants. Herein, ethyl acetate, methanol and water extracts from the Heracleum humile plant were obtained by an ultrasonic‐assisted extraction process and the aim was to evaluate some biological effects of the extracts due to the limited data on the pharmacological properties of Heracleum humile in the literature. Weak antibacterial activity was observed on tested bacterial species. The minimum inhibitory concentration and the minimum bactericidal concentration values ranged from 250 to 500 μg/mL. In addition, cytotoxic activity was determined using the MTT test. The strongest findings were determined for ethyl acetate extract on the MDA‐MB‐231 cell lines at the 48th hour (IC50:97.94 μg/mL), followed by the MCF‐7 cell lines at the 24th hour (IC50:103.9 μg/mL). All extracts of Heracleum humile contained mainly flavonoids, phenolic acids and their derivatives, i. e., well‐known compounds that possess numerous biological activities such as antioxidant, anti‐inflammatory, anticancer, antimicrobial etc. The study results could provide important information that Heracleum humile could be a potential candidate as a natural enzyme inhibitor. It can be concluded that these extracts could be useful in the elementary step of improving novel plant‐derived multifunctional pharmaceuticals.

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Comprehensive evaluation of two Astragalus species (A. campylosema and A. hirsutus) based on biological, toxicological properties and chemical profiling

Cited by 13 publications

References 47 publications

Clinical Study on the Treatment of Female Stress Urinary Incontinence With Modified Buzhong Yiqi Decoction

Clinical Study on the Treatment of Female Stress Urinary Incontinence With Modified Buzhong Yiqi Decoction

An In-Depth Study on the Metabolite Profile and Biological Properties of Primula auriculata Extracts: A Fascinating Sparkle on the Way from Nature to Functional Applications

Comparative Content, Biological and Anticancer Activities of Heracleum humile Extracts Obtained by Ultrasound‐Assisted Extraction Method

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