Comprehensive Evaluation of the Anti-Helicobacter pylori Activity of Scutellariae Radix

Abstract

− The aim of this study was to evaluate the anti-Helicobacter pylori activity of fractions and major aglycon compounds (baicalein, chrysin, oroxylin A, wogonin) of Scutellariae Radix. Minimum inhibitory concentration (MIC) measurement, DPPH radical-scavenging assay, DNA protection assay, and urease inhibition analysis were performed. The ethyl acetate (EtOAc) fraction showed the potent anti-Helicobacter activity, and therefore, compounds in the EtOAc fraction were subjected to further assay. The MICs of chrysin, oroxylin A, and wogonin against Helicobacter pylori 26695 were 6.25, 12.5 and 25 µg/mL, respectively. Baicalein exhibited the most effective DPPH radical-scavenging activity. DNA protection using Fenton reaction, chrysin, oroxylin A, and wogonin showed effective DNA protective effect. This result was also confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Regarding Jack bean urease (0.5 mg/mL, 50 unit/mg) inhibition, 20 mM ofbaicalein and chrysin inhibited urease activity by 88.2% and 72.5%, respectively.

Recent advances in design of new urease inhibitors: A review

“…5 , compounds 16 and 17 ). Baicalin was found to be a competitive, slow-binding and concentration-dependent inhibitor of jack bean and H. pylori ureases [66] , [67] , [68] . Kaempferol-3-O-β-D-glucopyranoside (compound 18 ) and kaempferol-3-O-α-L-rhamnopyranoside ( Fig.…”

Flavonoids and related privileged scaffolds as potential urease inhibitors: a review

“…12 , compounds 85 and 86). 95 It was discovered that baicalin acts as a competitive, slow-binding, and concentration-dependent blocker of ureases produced by both jack bean and H. pylori . 96 Sequestered from the fruits of Syzygium alternifolium , glucopyranoside 87 and rhamnopyranoside 88 ( Fig.…”