Comprehensive evaluation of SCFA production in the intestinal bacteria regulated by berberine using gas-chromatography combined with polymerase chain reaction

Wang, Lulu; Guo, Huihui; Huang, Shuai; Feng, Chen‐Lin; Han, Yan‐Xing; Jiang, Jian‐Dong

doi:10.1016/j.jchromb.2017.05.004

Cited by 45 publications

(26 citation statements)

References 62 publications

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“…Cecal and colon fecal samples for SCFAs analysis was prepared by centrifuging the suspension of 0.2 g feces and 1 mL PBS (pH 7.4) at 12 000 g for 10 min. Then, the concentrations of acetate, propionate, and butyrate of plasma and fecal samples were detected by gas chromatographic (GC) method described previously …”

Section: Methodsmentioning

confidence: 99%

Dual‐Stimuli‐Responsive Gut Microbiota‐Targeting Berberine‐CS/PT‐NPs Improved Metabolic Status in Obese Hamsters

Guo

Zheng

et al. 2019

Adv Funct Materials

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Gut dysbiosis has been found to be involved in the pathogenesis of energy metabolic disorders and might be a new strategy for these ailments. Berberine (BBR), a botanical medicine, shows therapeutic efficacy in patients with metabolic diseases. Numerous reports have shown BBR's modulating effect on gut microbiota, opening a new avenue to understand BBR's mechanism. In this study, a colon‐specific delivery system, BBR‐CS/PT‐NP, is investigated by the assembly of pH/gut microflora dual stimuli‐responsive nanoparticles for enhancing the interaction between BBR and gut microbiota. After oral administration, the delivering system remains stable in the stomach and small intestine, followed by a burst release of BBR after reaching the colon segment rich in intestinal bacteria. The enzymes produced by bacteria degrade the nanoparticle, causing direct exposure of BBR to gut microbiota. In the high fat diet‐induced obese hamsters, BBR‐CS/PT‐NP intervention inhibits weight‐gain and fat deposition, decreases plasma lipids and glucose levels, improves inflammation condition and insulin resistance, alleviates hepatic steatosis, at a level significantly higher than the pure BBR does. The mechanisms might be attributable to the enhanced interaction between BBR and the gut flora. The results provide a novel proof‐of‐concept for drug delivery targeting gut microbiota to ameliorate metabolic disorders.

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Section: Methodsmentioning

confidence: 99%

Dual‐Stimuli‐Responsive Gut Microbiota‐Targeting Berberine‐CS/PT‐NPs Improved Metabolic Status in Obese Hamsters

Guo

Zheng

et al. 2019

Adv Funct Materials

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“…This could be due to the fact that acidification decreases the interactions between SCFAs and other substances present in the sample (Tomaino, Parker, & Larick, 2001). Previous studies using formic acid for SCFAs analyses were applied on the samples of feces (Chen et al., 2019; García‐Villalba et al., 2012; Liebisch et al., 2019; Zhao, Nyman, & Åke Jönsson, 2006) and bacteria (Wang et al., 2017). Our research is the first report to apply formic acid for SCFAs analyses on the plasma samples in which SCFAs are very important components for use as health indices.…”

Section: Resultsmentioning

confidence: 99%

“…Short‐chain fatty acids (SCFAs) are mainly fermentation products produced by gut microbiota. These are a class of carboxylic acids with short two to six carbons alkyl chains, which influence metabolism and immune responses (Wang et al., 2017). In recent years, SCFAs have become a popular research topic, mainly because the concentration of SCFAs in the organism is closely related to diseases such as obesity, cardiovascular disease, and metabolic syndrome.…”

Section: Introductionmentioning

confidence: 99%

“…Gas chromatography (GC) combined with polymerase chain reaction (Wang et al., 2017), liquid chromatography‐tandem mass spectrometry (Zeng & Cao 2018), gas chromatography/mass spectrometry (GC/MS) (Mulat & Feilberg 2015; Amer et al., 2015), Gas chromatography‐flame ionization detection (GC‐FID) (Zhang, Wang & Liu 2015), and the solid‐phase micro extraction (SPME)‐GC‐MS (Douny et al., 2019) methods have been used either directly or after derivatization to analyze SCFAs. Prior to GC analysis, the sample preparation processes as described in the previous studies commonly included solvent extraction, phase separation, evaporation to complete dryness, saponification under heat, and the addition of internal standards (Gao et al., 2018; Lu et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

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Fast quantification of short‐chain fatty acids in rat plasma by gas chromatography

Wang

Chen

Tain

et al. 2020

Journal of Food Science

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Short‐chain fatty acids (SCFAs) are the main metabolites of the intestinal flora and play an important role in the interaction between the intestinal flora and host metabolism. Therefore, reliable methods are needed to accurately measure SCFAs concentrations. SCFAs are commonly analyzed by gas chromatography–mass spectrometry (GC‐MS), which requires lengthy sample treatments and a long run time. This study aimed to develop a fast GC method with formic acid pretreatment for SCFAs quantification in the plasma of rat. Baseline chromatographic resolution was achieved for three SCFAs (acetic, propionic, and butyric) within an analysis time of 10.5 min. The method exhibited good recovery for a wide range of concentrations with a low limit of detection for each compound. The relative standard deviations (RSDs) of all targeted compounds showed good intra‐ and interday precision (<10%). We used our method to measure SCFAs levels in plasma samples from rats fed with a high fructose diet (HFD) to test the accuracy of the developed method. It was shown that SCFAs are indeed affected negatively by a HFD (60% fructose). This method was successfully employed to accurately determine SCFAs in the rat plasma with minimum sample preparation. Results showed potential damage of HFD, which produced lower SCFAs. Practical Application Increasingly, microbiota and gut health research are being conducted by many food scientists to elucidate the relationships among the factors of food components, particularly the nondigestible carbohydrates, food processing conditions, and potential health impact. This research provides a useful, rapid, and accurate method that can save time in the analysis of short‐chain fatty acids, which are commonly analyzed in gut health research.

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“…The suspension was filtered through a 1‐mm sieve. The growth medium was as follows: arabinogalactan (1 g/L), pectin (2 g/L), xylan (1 g/L), starch (4.2 g/L), glucose (0.4 g/L), yeast extract (3 g/L), peptone (1 g/L), mucin (4 g/L), and cysteine (0.5 g/L) (35).…”

Section: Methodsmentioning

confidence: 99%

The gut microbiota modulator berberine ameliorates collagen‐induced arthritis in rats by facilitating the generation of butyrate and adjusting the intestinal hypoxia and nitrate supply

Yue

Yu²,

Fang³

et al. 2019

The FASEB Journal

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The commensal microbiota is one of the environmental triggers of rheumatoid arthritis (RA). Recent studies have identified the characteristics of the gut microbiota in patients with RA. However, it is still unclear how the microbiota can be modulated to slow down disease progression. In the present study, berberine, a modulator of gut microbiota with substantial anti‐RA effect, was chosen to explore the mechanisms by which the microbiota modulators ameliorate RA. The results showed that oral administration of berberine alleviated collagen‐induced arthritis (CIA) in rats in a gut microbiota‐dependent manner. Berberine down‐regulated the diversity and richness of the gut bacteria, reduced the abundance of Prevotella, and elevated the abundance of butyrate‐producing bacteria in CIA rats as determined by the 16S rRNA gene sequence, which might function through limiting the generation of nitrate and stabilizing the physiologic hypoxia in the intestine. Moreover, berberine treatment significantly increased the intestinal butyrate level and promoted the expression and activity of butyryl‐CoA:acetate‐CoA transferase (BUT). The coadministration of a BUT inhibitor largely diminished the adjustment of intestinal environment and the antiarthritic effect of berberine. In conclusion, modulators of the gut microbiota might serve as therapeutic agents for RA by inducing the butyrate generation through promoting the expression and activity of BUT.—Yue, M., Tao, Y., Fang, Y., Lian, X., Zhang, Q., Xia, Y., Wei, Z., Dai, Y. The gut microbiota modulator berberine ameliorates collagen‐induced arthritis in rats by facilitating the generation of butyrate and adjusting the intestinal hypoxia and nitrate supply. FASEB J. 33,12311‐12323 (2019). http://www.fasebj.org

show abstract

Comprehensive evaluation of SCFA production in the intestinal bacteria regulated by berberine using gas-chromatography combined with polymerase chain reaction

Cited by 45 publications

References 62 publications

Dual‐Stimuli‐Responsive Gut Microbiota‐Targeting Berberine‐CS/PT‐NPs Improved Metabolic Status in Obese Hamsters

Dual‐Stimuli‐Responsive Gut Microbiota‐Targeting Berberine‐CS/PT‐NPs Improved Metabolic Status in Obese Hamsters

Fast quantification of short‐chain fatty acids in rat plasma by gas chromatography

The gut microbiota modulator berberine ameliorates collagen‐induced arthritis in rats by facilitating the generation of butyrate and adjusting the intestinal hypoxia and nitrate supply

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