Comprehensive estrogenic/anti-estrogenic, anticancer, mutagenic/anti-mutagenic, and genotoxic/anti-genotoxic activity studies on chemically characterized black poplar and Eurasian aspen propolis types

Güzelmeriç, Etil; Sipahi, Hande; Özhan, Yağmur; Hamitoğlu, Muhammed; Helvacıoğlu, Sinem; Düz, Gamze; Akyıldız, İsmail Emir; Yaman, Beril Kadıoğlu; Hazar, Merve; Dilsiz, Sevtap Aydın; Aydın, Ahmet; Yeşilada, Erdem

doi:10.1016/j.jpba.2023.115241

Cited by 7 publications

(8 citation statements)

References 29 publications

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“…Chromatographic identification of the ingredients showed the presence of the same active compounds as in propolis from urban apiaries. In Turkish propolis, which is also of the poplar type, the presence of the same chemical components as in Polish urban propolis, i.e., pinocembrin, chrysin, pinobanksin, and galangin, as the dominant flavonoids, as well as, ferulic acid, p-coumaric acid, and trans-cinnamic acid as the dominant phenolic acids [ 27 ], which confirms that propolis from urban apiaries does not differ significantly from propolis from apiaries located in agricultural areas. As a result of aerobic cellular respiration, by-products are generated in the form of free radicals.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Chemical Composition and Antioxidant Properties of Propolis from Urban Apiaries

Pobiega,

Kot,

Przybył

et al. 2023

Molecules

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Bee products from urban apiaries are increasingly used. They are mainly used to promote local apiaries and cities in which they are located. The aim of the study was to compare the chemical composition and antioxidant activity of propolis from 6 Polish apiaries located in cities (Legionowo, Torun, Cracow, Warsaw, Katowice, Lodz). The chemical composition was analyzed using liquid chromatography (HPLC-DAD) and the analysis of antioxidant activity by scavenging free radicals (ABTS and DPPH) and FRAP. The obtained results showed the presence of 24 phenolic compounds in propolis extracts. The tested samples showed differentiation in terms of the content of individual chemical components, however, cinnamic acid and its derivatives were dominant. High antioxidant activity of the tested extracts was demonstrated (ABTS was in the range of 16.80–51.53 mg Te/mL, DPPH was in the range of 7.54–22.13 mg Te/mL, while FRAP reduction was in the range of 10.93–29.55 mg Te/mL). The obtained results compared with literature data on propolis from agricultural areas allow to conclude that propolis samples from both Poland types of areas are similar and can be classified as poplar propolis.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Chemical Composition and Antioxidant Properties of Propolis from Urban Apiaries

Pobiega,

Kot,

Przybył

et al. 2023

Molecules

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“…The individual phenolics and their concentration in the propolis extracts (EWE, PGE, and BE) were given in Table 1 . The phenolic profiles of samples were typical for poplar propolis with the presence of flavonoids such as CAPE, pinocembrin, pinobaksin, galangin, apigenin, chrysin, quercetin, and phenolic acids such as ferulic acid, p‐coumaric acid, and cinnamic acid (Guzelmeric et al., 2023 ; Ozdal et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…The phenolic profiles of undigested and digested samples was determined according to the method of Guzelmeric et al. ( 2023 ) by using Waters® Xevo (Waters Corporation, Milford, MA, USA) LC–MS/MS system equipped with a binary pump, a temperature‐controlled column compartment, an autosampler, and a triple‐quadrupole (TQ) MS/MS mass analyzer with ESI ion source. An analytical Cortecs T3 (Waters Corporation Milford, USA) column (2.1 × 150 mm, 1.6 μm) was used for the separation.…”

Section: Methodsmentioning

confidence: 99%

The evaluation of L‐arginine solution as a solvent for propolis extraction: The phenolic profile, antioxidant, antibacterial activity, and in vitro bioaccessibility

Mergen Duymaz,

Duz,

Ozkan

et al. 2024

Food Science & Nutrition

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Ethanol has been widely used for the extraction of propolis. Due to its certain disadvantages, there has been an ongoing search to find alternative non‐ethanolic extraction solvents. This study aimed to compare the phenolics, antioxidant, and antibacterial activity of propolis extracts prepared with 70% ethanol (EWE), propylene glycol (PGE), and L‐arginine solution (BE). All extracts were subjected to an in vitro simulated digestion procedure, and the phenolic profile of non‐digested and digested samples was determined by using LC–MS/MS. Additionally, the change in total phenolic (TPC), total flavonoid content (TFC), and antioxidant capacities were determined at each digestion phase. TPC and TFC of non‐digested propolis extracts had similar values, although BE showed higher antioxidant capacity (p < .05). The amount of TPC reached or transformed at the intestinal stage was higher for BE and PG compared to EWE. BE also provided the highest antioxidant capacity assay in digested samples. The most common phenolics were pinocembrin, pinobanskin, galangin, and CAPE in non‐digested extracts. However, their concentration was drastically reduced by digestion, and their recovery (R%) ranged from 0% to 9.38% of the initial amount detected in the non‐digested extracts. Chrysin was the most bioaccessible flavonoid in all extracts. Among phenolic acids, the highest R% was determined for trans‐cinnamic acid (22.14%) from BE. All extracts showed in vitro inhibitory activity against Escherichia coli and Staphylococcus aureus. This study suggests that an L‐arginine solution could be used as an alternative solvent to ethanol and propylene glycol for propolis extraction.

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“…LC-MS/MS analysis was performed according to the method of Guzelmeric et al [ 2 ]. Identification of polyphenol was performed with a Waters Acquity UPLC H-class system coupled to a Waters Xevo TQD triple quadrupole mass spectrometer (Waters Corporation, Milford, MA USA) equipped with an electrospray ionization (ESI) source.…”

Section: Methodsmentioning

confidence: 99%

“…Propolis, a natural resinous beehive product, exhibits diverse biological and pharmacological activities due to the presence of flavonoids, phenolic acids, terpenes, sesquiterpene-alcohols, and their derivatives [ 1 ]. Specifically, among flavonoids, the caffeic acid phenyl ester (CAPE), chrysin, galangin, apigenin, quercetin, kaempferol, naringenin, rutin, taxifolin, pinobanksin, and pinocembrin have been reported as the major active components of propolis [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Propolis-loaded liposomes: characterization and evaluation of the in vitro bioaccessibility of phenolic compounds

Karadag,

Saroglu

2024

ADMET DMPK

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Background and purpose: Propolis has low water solubility, poor stability, and limited bioaccessibility of phenolic constituents when subjected to in vitro digestion. To overcome these drawbacks, the liposomal encapsulation method can be employed. Experimental approach: Soybean phosphatidylcholine lecithin mixed with Tween 80 (T80) and ammonium phosphatides (AMP) was used to produce propolis extract (PE)-loaded liposomes. The mean particle size, zeta potential, encapsulation efficiency values, and transmission electron microscopy analysis were used to characterize liposomes. Individual phenolics were determined for digested and nondigested propolis-loaded liposomes and propolis extract. Key results: Tween 80 incorporation reduced the size of unloaded liposomes, whereas AMP inclusion yielded larger liposomes. In both formulations, PE loading significantly increased the size and reduced the zeta potential values and homogeneity of the size distribution. In free PE, the most bioaccessible polyphenols were phenolic acids (3.20 to 5.63 %), and flavonoids such as caffeic acid phenethyl ester, galangin, pinobanksin, and pinocembrin (0.03 to 2.12 %) were the least bioaccessible. Both liposomal propolis provided significantly higher bioaccessibility of phenolic compounds. The liposomes with T80 and AMP in their compositions recovered 52.43 and 185.90 % of the total amount of phenolic compounds in the nondigested samples, respectively. The liposomes containing AMP not only exhibited high solubility for PE but also provided protection to the phenolic compounds during in vitro digestion. Conclusion: Liposomal encapsulation could be a promising approach to improving the solubility and stability of PE in digestive fluids, making it suitable for the delivery of propolis in oral formulations.

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Comprehensive estrogenic/anti-estrogenic, anticancer, mutagenic/anti-mutagenic, and genotoxic/anti-genotoxic activity studies on chemically characterized black poplar and Eurasian aspen propolis types

Cited by 7 publications

References 29 publications

Comparison of the Chemical Composition and Antioxidant Properties of Propolis from Urban Apiaries

Comparison of the Chemical Composition and Antioxidant Properties of Propolis from Urban Apiaries

The evaluation of L‐arginine solution as a solvent for propolis extraction: The phenolic profile, antioxidant, antibacterial activity, and in vitro bioaccessibility

Propolis-loaded liposomes: characterization and evaluation of the in vitro bioaccessibility of phenolic compounds

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