Comprehensive co-expression analysis provides novel insights into temporal variation of flavonoids in fresh leaves of the tea plant (Camellia sinensis)

Zhu, Junyan; Xu, Qingshan; Zhao, Sheng; Xia, Xiaobo; Yan, Xiaomei; An, Yanlin; Mi, Xiaozeng; Guo, Lingxiao; Самарина, Л. С.; Wei, Chaoling

doi:10.1016/j.plantsci.2019.110306

Cited by 56 publications

(37 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that there is a rhythm, a time-distribution character, to the biosynthesis and metabolism of flavonoids in Ginkgo leaves (Cheng et al, 2012;Sati et al, 2013). Our results indicated that flavonoid-related metabolism was more active at the transcriptional level in the early stage of leaf development, consistent with previous studies (Yan et al, 2019;Zhu et al, 2020). These findings were also confirmed by the result of HPLC analysis; where we found that the content of TFG peaked in the early stage ( Figure 1A).…”

Section: Flavonoids Metabolism Is Temporospatially Influencedsupporting

confidence: 92%

“…More specifically, we identified one gene (Gb_41796) encoding PAL, which is an upstream key enzyme and rate limiting of the flavonoids biosynthesis pathway (Wang et al, 2014), whose expression was positively correlated with the abundance of three flavones (luteolin, apigenin, and vitexin), but negatively correlated with the two isoflavones (daidzin and genistein). Additionally, we found two genes encoding UGT (Gb_24439 and Gb_26148) whose expression was significantly and positively correlated with the abundance of flavonoid glycosides (astragalin), consistent with and supporting previous studies (Cui et al, 2016;Zhu et al, 2020). The accumulation of anthocyanins has been reported to be positively correlated with the expression of GbDFRs (Ni et al, 2020), while we found the gene encoding DFR (Gb_09086) was negatively associated with cyanidin accumulation.…”

Section: A Regulated Transcriptional Network For Flavonoids Biosynthesissupporting

confidence: 91%

See 1 more Smart Citation

Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves

Guo

Wang

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

Ginkgo (Ginkgo biloba L.) is a high-value medicinal tree species characterized by its flavonoids beneficial effects that are abundant in leaves. We performed a temporospatial comprehensive transcriptome and metabolome dynamics analyses of clonally propagated Ginkgo plants at four developmental stages (time: May to August) across three different environments (space) to unravel leaves flavonoids biosynthesis variation. Principal component analysis revealed clear gene expression separation across samples from different environments and leaf-developmental stages. We found that flavonoid-related metabolism was more active in the early stage of leaf development, and the content of total flavonoid glycosides and the expression of some genes in flavonoid biosynthesis pathway peaked in May. We also constructed a co-expression regulation network and identified eight GbMYBs and combining with other TF genes (3 GbERFs, 1 GbbHLH, and 1 GbTrihelix) positively regulated the expression of multiple structural genes in the flavonoid biosynthesis pathway. We found that part of these GbTFs (Gb_11316, Gb_32143, and Gb_00128) expressions was negatively correlated with mean minimum temperature and mean relative humidity, while positively correlated with sunshine duration. This study increased our understanding of the molecular mechanisms of flavonoids biosynthesis in Ginkgo leaves and provided insight into the proper production and management of Ginkgo commercial plantations.

show abstract

Section: Flavonoids Metabolism Is Temporospatially Influencedsupporting

confidence: 92%

Section: A Regulated Transcriptional Network For Flavonoids Biosynthesissupporting

confidence: 91%

Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves

Guo

Wang

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…Previous studies have elucidated a consistent regulatory mechanism in tea plants. For instance, CsbHLH (TEA003964) acts as a potential repressor to negatively regulate the expression of downstream flavonoid synthesis genes in tea plants [46]. CsMYB5a and CsMYB5e were also demonstrated to be involved in the biosynthesis regulation of multiple flavonoid components [47].…”

Section: Discussionmentioning

confidence: 99%

Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics

Wang

Chen

et al. 2020

IJMS

View full text Add to dashboard Cite

Blue light extensively regulates multiple physiological processes and secondary metabolism of plants. Although blue light quantity (fluence rate) is important for plant life, few studies have focused on the effects of different blue light intensity on plant secondary metabolism regulation, including tea plants. Here, we performed transcriptomic and metabolomic analyses of young tea shoots (one bud and two leaves) under three levels of supplemental blue light, including low-intensity blue light (LBL, 50 μmol m–2 s–1), medium-intensity blue light (MBL, 100 μmol m–2 s–1), and high-intensity blue light (HBL, 200 μmol m–2 s–1). The total number of differentially expressed genes (DEGs) in LBL, MBL and HBL was 1, 7 and 1097, respectively, indicating that high-intensity blue light comprehensively affects the transcription of tea plants. These DEGs were primarily annotated to the pathways of photosynthesis, lipid metabolism and flavonoid synthesis. In addition, the most abundant transcription factor (TF) families in DEGs were bHLH and MYB, which have been shown to be widely involved in the regulation of plant flavonoids. The significantly changed metabolites that we detected contained 15 lipids and 6 flavonoid components. Further weighted gene co-expression network analysis (WGCNA) indicated that CsMYB (TEA001045) may be a hub gene for the regulation of lipid and flavonoid metabolism by blue light. Our results may help to establish a foundation for future research investigating the regulation of woody plants by blue light.

show abstract

“…Ginkgo biloba , one of relic plant species, is looked as one living fossil 14 , contains flavonoids and terpenoids that affect antioxidant activities, platelet-activating factors, peripheral blood vessels, and blood circulation 15 . Flavonoids are synthesized by the combination of the phenylpropanoid and polyketide pathways 16 .Transcription factor were involved in flavonoid biosynthesis by regulating expression of structural genes 17 . Some structural genes related to flavonoid biosynthesis were cloned and characterized from G. biloba , including phenylalanine ammonia-lyase ( PAL ) 18 , flavonol synthase ( FLS ) 19 , flavanone 3-hydroxylase ( F3H ) 20 , chalcone synthase ( CHS ) 21 , chalcone isomerase ( CHI ) 22 , isoflavone reductase-like ( IFR-like ) 23 , dihydroflavonol-4-reductase ( DFR ) 24 , anthocyanidin reductase ( ANR ) 25 , anthocyanidin synthase ( ANS ) 26 , cinnamate‑4‑hydroxylase ( C4H ) 27 .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of bHLH family genes from Ginkgo biloba

Zhou

Liao

Kim

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Basic helix–loop–helix (bHLH) proteins, one of the most important and largest transcription factor family in plants, play important roles in regulating growth and development, stress response. In recent years, many bHLH family genes have been identified and characterized in woody plants. However, a systematic analysis of the bHLH gene family has not been reported in Ginkgo biloba , the oldest relic plant species. In this study, we identifed a total of 85 GbbHLH genes from the genomic and transcriptomic databases of G. biloba , which were classified into 17 subfamilies based on the phylogenetic analysis. Gene structures analysis indicated that the number of exon–intron range in GbbHLHs from 0 to 12. The MEME analysis showed that two conserved motifs, motif 1 and motif 2, distributed in most GbbHLH protein. Subcellular localization analysis exhibited that most GbbHLHs located in nucleus and a few GbbHLHs were distributed in chloroplast, plasma membrane and peroxisome. Promoter cis-element analysis revealed that most of the GbbHLH genes contained abundant cis-elements that involved in plant growth and development, secondary metabolism biosynthesis, various abiotic stresses response. In addition, correlation analysis between gene expression and flavonoid content screened seven candidate GbbHLH genes involved in flavonoid biosynthesis, providing the targeted gene encoding transcript factor for increase the flavonoid production through genetic engineering in G. biloba .

show abstract

Comprehensive co-expression analysis provides novel insights into temporal variation of flavonoids in fresh leaves of the tea plant (Camellia sinensis)

Cited by 56 publications

References 43 publications

Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves

Temporospatial Flavonoids Metabolism Variation in Ginkgo biloba Leaves

Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics

Genome-wide identification and characterization of bHLH family genes from Ginkgo biloba

Contact Info

Product

Resources

About