Comprehensive circRNA/miRNA/mRNA Analysis Reveals circRNAs Protect Against Toxicity Induced by BPA in GC-2 Cells

Li, Huimin; Dai, Yuwan; Yu, Jiangyu; Duan, Peng; Ma, Xiaoxin; Dong, Wei; Li, Na; Li, Honggang

doi:10.2217/epi-2018-0217

Cited by 22 publications

(15 citation statements)

References 46 publications

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“…Analysis of circRNA–miRNA–mRNA coexpression networks is vital in the preliminary prediction of circRNA function and can also implicate the genes involved in core biological functions 34 . A ceRNA network of 12 screened mRNAs (Table 2) was constructed, and we found that eight mRNAs (Pln, Fabp3, Cdh2, Cdk1, Fxyd1, Nprl3, Dkk3 and Cldn15) and five circRNAs (mmu_circ_0001679, mmu_circ_0001212, mmu_circ_0000372, mmu_circ_0001471 and mmu_circ_0000723) may contribute to P2X 7 R antagonism via A‐438079.…”

Section: Discussionmentioning

confidence: 99%

“…Analysis of circRNA-miRNA-mRNA coexpression networks is vital in the preliminary prediction of circRNA function and can also implicate the genes involved in core biological functions. 34 A F I G U R E 6 Validation of eight screened mRNAs by RT-qPCR. Expression patterns of eight genes were consistent with the sequencing results.…”

Section: Verification Of Dysregulated Circrnas and Mrnasmentioning

confidence: 99%

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Protective effects of P2X7R antagonist in sepsis‐induced acute lung injury in mice via regulation of circ_0001679 and circ_0001212 and downstream Pln, Cdh2, and Nprl3 expression

Zou

Wang

Zhou

et al. 2020

The Journal of Gene Medicine

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Background: Sepsis induces pulmonary P2X 7 receptor (P2X 7 R) expression and P2X 7 R-knockout reduced lung inflammation in mice. The present study investigated the expression of circular RNA (circRNA) and mRNA in sepsis-induced acute lung injury (ALI) treated with a P2X 7 R antagonist. Methods: Sepsis was induced by tracheal administration of lipopolysaccharide (LPS), and the mice were then divided into two groups: without [sepsis + dimethyl sulfoxide (DMSO)] or with P2X 7 R antagonist treatment (sepsis + P2X 7 A). Sham mice were administrated sterile normal saline. Serum levels of interleukin (IL)-1β and tumor necrosis factor (TNF)-α, pathological changes, cell apoptosis and P2X 7 R expression in lung were assessed, followed by RNA sequencing (RNA-seq) and bioinformatics analyses. A quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to validate circRNAs and mRNAs. Results: Compared to the sham group, LPS-induced sepsis produced obvious pathological changes in lung tissue, as well as increased apoptotic lung cells, serum TNF-α and IL-1β levels, and P2X 7 R expression; P2X 7 R antagonism significantly ameliorated these changes. RNA-seq identified many dysregulated circRNAs and mRNAs during sepsis, whereas this changed with P2X 7 R antagonism. RT-qPCR confirmed that Mus musculus (mmu)_circ_0001679, mmu_circ_0001212, phospholamban (Pln), cadherin-2 (Cdh2) and nitrogen permease regulator 3-like (Nprl3) expression were significantly increased in the sepsis + DMSO group compared to that in the sham group but were decreased in the sepsis + P2X 7 A group compared to that in the sepsis + DMSO group. The circRNA-microRNA-mRNA coexpression network indicated that mmu_circ_0001679 may regulate Nprl3 and that mmu_circ_0001212 may similarly regulate Pln, Cdh2 and Nprl3 as a competing endogenous RNA. Conclusions: P2X 7 R antagonism attenuates sepsis-induced ALI by inhibiting dysregulated expression of circRNA (circ_0001679, circ_0001212) and mRNA (Pln, Cdh2 and Nprl3).

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Section: Discussionmentioning

confidence: 99%

Section: Verification Of Dysregulated Circrnas and Mrnasmentioning

confidence: 99%

Protective effects of P2X7R antagonist in sepsis‐induced acute lung injury in mice via regulation of circ_0001679 and circ_0001212 and downstream Pln, Cdh2, and Nprl3 expression

Zou

Wang

Zhou

et al. 2020

The Journal of Gene Medicine

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“…By using paired-end sequencing, the nucleotide length of sequencing was 150 bp. Single-stranded DNA was generated using 0.1 M NaOH and its loading concentration was 8 pM, which was measured by qPCR (11,12). Sequencing quality was evaluated by FastQC v0.11.7 software (http://www.bioinformatics.…”

Section: Methodsmentioning

confidence: 99%

Circular RNA expression profile in human primary multiple intracranial aneurysm

et al. 2021

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Primary multiple intracranial aneurysm (MIA) is a vascular disease that frequently leads to fatal vascular rupture and subarachnoid hemorrhage. However, the epigenetic regulation associated with MIA has remained largely elusive. Circular RNAs (circRNAs) serve important roles in cardiovascular diseases; however, their association with MIA has remained to be investigated. The present study initially aimed to explore novel mechanisms of MIA through examining circRNA expression profiles. Comprehensive circRNA expression profiles were detected by RNA sequencing (RNA-Seq) in human peripheral blood mononuclear cells. The RNA-Seq results were validated by reverse transcription-quantitative PCR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses suggested the functions of these circRNAs. A competing endogenous RNA network was constructed to reveal the circRNA-microRNA-mRNA relationship. Among the 3,328 differentially expressed circRNAs between the MIA and matched control groups, 60 exhibited significant expression changes (|log2 fold change|≥2; P<0.05). Among these 60 circRNAs, 20 were upregulated, while the other 40 were downregulated. A number of downregulated circRNAs were involved in inflammation. The most significant KEGG pathway was ‘leukocyte transendothelial migration’. The circRNAs Homo sapiens (hsa)_circ_0135895, hsa_circ_0000682 and hsa_circ_0000690, which were also associated with the above-mentioned pathway, were indicated to be able to regulate protein tyrosine kinase 2, protein kinase Cβ and integrin subunit αL, respectively. To the best of our knowledge, the present study was the first to perform a circRNA sequencing analysis of MIA. The results specifically predicted the regulatory role of circRNAs in the pathogenesis of MIA. ‘Leukocyte transendothelial migration’ may be critical for the pathogenesis of MIA.

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“…A testis-specific circRNA derived from male sex-determining gene Sry was shown to contain 16 sites for miR-138, acting as its sponge [ 132 ]. Furthermore, circRNAs were shown to respond to toxicity and stress in the germline and may play protective roles in germline development by sponging miRNAs [ 133 , 134 , 135 ].…”

Section: Functions Of Sncrnas During Mammalian Germ Cell Developmentmentioning

confidence: 99%

Small Noncoding RNAs in Reproduction and Infertility

et al. 2021

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Infertility has been reported as one of the most common reproductive impairments, affecting nearly one in six couples worldwide. A large proportion of infertility cases are diagnosed as idiopathic, signifying a deficit in information surrounding the pathology of infertility and necessity of medical intervention such as assisted reproductive therapy. Small noncoding RNAs (sncRNAs) are well-established regulators of mammalian reproduction. Advanced technologies have revealed the dynamic expression and diverse functions of sncRNAs during mammalian germ cell development. Mounting evidence indicates sncRNAs in sperm, especially microRNAs (miRNAs) and transfer RNA (tRNA)-derived small RNAs (tsRNAs), are sensitive to environmental changes and mediate the inheritance of paternally acquired metabolic and mental traits. Here, we review the critical roles of sncRNAs in mammalian germ cell development. Furthermore, we highlight the functions of sperm-borne sncRNAs in epigenetic inheritance. We also discuss evidence supporting sncRNAs as promising biomarkers for fertility and embryo quality in addition to the present limitations of using sncRNAs for infertility diagnosis and treatment.

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Comprehensive circRNA/miRNA/mRNA Analysis Reveals circRNAs Protect Against Toxicity Induced by BPA in GC-2 Cells

Cited by 22 publications

References 46 publications

Protective effects of P2X7R antagonist in sepsis‐induced acute lung injury in mice via regulation of circ_0001679 and circ_0001212 and downstream Pln, Cdh2, and Nprl3 expression

Protective effects of P2X7R antagonist in sepsis‐induced acute lung injury in mice via regulation of circ_0001679 and circ_0001212 and downstream Pln, Cdh2, and Nprl3 expression

Circular RNA expression profile in human primary multiple intracranial aneurysm

Small Noncoding RNAs in Reproduction and Infertility

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