Comprehensive chemical and metabolic profiling of anti‐hyperglycemic active fraction from Clerodendranthi Spicati Herba

Luo, Yun; Liu, Yue; Wen, Quan; Feng, Yulin

doi:10.1002/jssc.202000834

Cited by 8 publications

(5 citation statements)

References 22 publications

(27 reference statements)

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“…In a recent study, the 95% ethanol elution fraction (95% EEF) of 80% ethanol extract (0.68 g/kg, 0.34 g/kg and 0.17 g/kg) reduced blood glucose levels in an oral glucose tolerance test in normal C57BL/6J mice after 10-day administration of the extract [39]. The ethanol extract of O. stamineus (0.2 and 0.4 g/kg) obviously reduced fasting blood glucose level in high-fat-diet (HFD) C57BL/6 mice after 8-week administration of the extract [40].…”

Section: Hypoglycemic Activitymentioning

confidence: 99%

“…In the conformation of α-glucosidase-rosmarinic acid complex, there were 6 hydrogen bonds [66]. The 95% EEF showed higher α-glucosidase (86 µg/mL) inhibitory activity (IC50 = 40 ± 0.73 µg/mL) than acarbose (IC50 = 250 ± 1.05 µg/mL) [39]. The ethanol extract (1000 µg/mL) of some genotypes of O. stamineus inhibited α-glucosidase up to 62.84% [46].…”

Section: Inhibit the Activities Of α-Amylase And α-Glucosidasementioning

confidence: 99%

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A Systematic Review of Orthosiphon stamineus Benth. in the Treatment of Diabetes and Its Complications

Wang

et al. 2022

Molecules

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(1) Background: Orthosiphon stamineus Benth. is a traditional medicine used in the treatment of diabetes and chronic renal failure in southern China, Malaysia, and Thailand. Diabetes is a chronic metabolic disease and the number of diabetic patients in the world is increasing. This review aimed to systematically review the effects of O. stamineus in the treatment of diabetes and its complications and the pharmacodynamic material basis. (2) Methods: This systematic review was conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), using the databases ScienceDirect, PubMed, and Web of Science. (3) Results: Thirty-one articles related to O. stamineus and diabetes were included. The mechanisms of O. stamineus in the treatment of diabetes and its complications mainly included inhibiting α-amylase and α-glucosidase activities, antioxidant and anti-inflammatory activities, regulating lipid metabolism, promoting insulin secretion, ameliorating insulin resistance, increasing glucose uptake, promoting glycolysis, inhibiting gluconeogenesis, promoting glucagon-likepeptide-1 (GLP-1) secretion and antiglycation activity. Phenolic acids, flavonoids and triterpenoids might be the main components for hypoglycemia effects in O. stamineus. (4) Conclusion: O. stamineus could be an antidiabetic agent to treat diabetes and its complications. However, it needs further study on a pharmacodynamic substance basis and the mechanisms of effective constituents.

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Section: Hypoglycemic Activitymentioning

confidence: 99%

Section: Inhibit the Activities Of α-Amylase And α-Glucosidasementioning

confidence: 99%

A Systematic Review of Orthosiphon stamineus Benth. in the Treatment of Diabetes and Its Complications

Wang

et al. 2022

Molecules

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“…One animal study has shown that the water extract of O. stamineus has hypoglycemic effects on diabetic rats [ 10 ]. In a recent study, 80% ethanol extract (about 0.4 g/kg) reduced blood glucose levels in an oral glucose tolerance test in normal C57BL/6J mice and high-fat-diet (HFD) C57BL/6 mice after 1.5 [ 11 ] and 8 weeks [ 12 ] administration of the extract, respectively. Water extract of O. stamineus was also administrated orally (0.5 g/kg) to normal and diabetic rats loaded with glucose.…”

Section: Introductionmentioning

confidence: 99%

In Vivo and In Vitro Antidiabetic Efficacy of Aqueous and Methanolic Extracts of Orthosiphon Stamineus Benth

et al. 2023

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Orthosiphon stamineus is a popular folk herb used to treat diabetes and some other disorders. Previous studies have shown that O. stamineus extracts were able to balance blood glucose levels in diabetic rat animal models. However, the antidiabetic mechanism of O. stamineus is not fully known. This study was carried out to test the chemical composition, cytotoxicity, and antidiabetic activity of O. stamineus (aerial) methanol and water extracts. GC/MS phytochemical analysis of O. stamineus methanol and water extracts revealed 52 and 41 compounds, respectively. Ten active compounds are strong antidiabetic candidates. Oral treatment of diabetic mice with O. stamineus extracts for 3 weeks resulted significant reductions in blood glucose levels from 359 ± 7 mg/dL in diabetic non-treated mice to 164 ± 2 mg/dL and 174 ± 3 mg/dL in water- and methanol-based-extract-treated mice, respectively. The efficacy of O. stamineus extracts in augmenting glucose transporter-4 (GLUT4) translocation to the plasma membrane (PM) was tested in a rat muscle cell line stably expressing myc-tagged GLUT4 (L6-GLUT4myc) using enzyme-linked immunosorbent assay. The methanol extract was more efficient in enhancing GLUT4 translocation to the PM. It increased GLUT4 translocation at 250 µg/mL to 279 ± 15% and 351 ± 20% in the absence and presence of insulin, respectively. The same concentration of water extract enhanced GLUT4 translocation to 142 ± 2.5% and 165 ± 5% in the absence and presence of insulin, respectively. The methanol and water extracts were safe up to 250 µg/mL as measured with a Methylthiazol Tetrazolium (MTT) cytotoxic assay. The extracts exhibited antioxidant activity as measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. O. stamineus methanol extract reached the maximal inhibition of 77 ± 10% at 500 µg/mL, and O. stamineus water extract led to 59 ± 3% inhibition at the same concentration. These findings indicate that O. stamineus possesses antidiabetic activity in part by scavenging the oxidants and enhancing GLUT4 translocation to the PM in skeletal muscle.

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“…Many different techniques have been developed to improve the identification of in vivo metabolites of complex components of TCMs, including mass defect filter, diagnostic product ion (DPI) filter, neutral loss (NL) filter and background subtraction. Usually, these techniques were used in combination of two or three to simplify and accelerate the identification of in vivo metabolites of complex components of TCMs 15–18 …”

Section: Introductionmentioning

confidence: 99%

“…Usually, these techniques were used in combination of two or three to simplify and accelerate the identification of in vivo metabolites of complex components of TCMs. [15][16][17][18] In the study reported here, we established an enhanced strategy integrating UHPLC-QTOF-MS/MS with a data filtering strategy, including DPI and NL filters, to systematically characterize the xenobiotics in plasma, urine and feces of rat after oral administration of FCAVA. Firstly, the complex biological samples were analyzed using our reported method.…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of the flower of Citrus aurantium L. var. amara Engl. in rats using ultra‐high‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry with data mining strategy

Zhou,

Huang,

Tan

et al. 2023

Rapid Comm Mass Spectrometry

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RationaleThe flower of Citrus aurantium L. var. amara Engl. (FCAVA), an edible tea and herbal medicine with anti‐obesity effect, has attracted great attention in China. The structural elucidation of chemical components in FCAVA has been realized in our previous work. It is well known that metabolic profiling provided a structural basis to discover potential anti‐obesity ingredients in FCAVA. Nevertheless, there are no reports about in vivo metabolic profiles of FCAVA. Therefore, it is necessary to comprehensively identify in vivo substances of FCAVA.MethodsThe identification of in vivo substances of FCAVA remains a challenge due to the strong interference of complex chemical components, biological matrices and metabolite isomers. In this work, ultra‐high‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry (UHPLC‐QTOF‐MS/MS) analysis with a data mining strategy was established and applied for the metabolic profiling of FCAVA in rats. The data mining strategy, including diagnostic product ions and neutral loss filtering, improved structural elucidation of xenobiotics in rats after oral administration of FCAVA.ResultsA total of 228 xenobiotics, including 80 prototypes (10 unambiguous confirmed with reference standards) and 148 metabolites, were tentatively characterized in rat plasma, urine and fecal samples. Among them, 35 xenobiotics were found in plasma, 124 in urine and 156 in feces. The main biotransformation pathway of FCAVA metabolism was deglycosylation, methylation, glucuronidation and sulfation. The main compounds absorbed into the blood were neohesperidin and naringin, which have been reported to show significant anti‐obesity effect.ConclusionsCollectively, this present study would be conducive to the discovery of active ingredients of FCAVA for the treatment of obesity and the development of quality control of FCAVA.

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Comprehensive chemical and metabolic profiling of anti‐hyperglycemic active fraction from Clerodendranthi Spicati Herba

Cited by 8 publications

References 22 publications

A Systematic Review of Orthosiphon stamineus Benth. in the Treatment of Diabetes and Its Complications

A Systematic Review of Orthosiphon stamineus Benth. in the Treatment of Diabetes and Its Complications

In Vivo and In Vitro Antidiabetic Efficacy of Aqueous and Methanolic Extracts of Orthosiphon Stamineus Benth

Metabolic profiling of the flower of Citrus aurantium L. var. amara Engl. in rats using ultra‐high‐performance liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry with data mining strategy

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