Comprehensive chemical analysis of Schisandra chinensis by HPLC–DAD–MS combined with chemometrics

Determination of lignans and phenolic components of Schisandra chinensis (Turcz.) Baill. using HPLC-ESI-ToF-MS and HPLC-online TEAC: Contribution of individual components to overall antioxidant activity and comparison with traditional antioxidant assays

“…Extracts were prepared from the fruits, leaves, and stems as previously described by Liu et al (2013), with slight modification. Methanol, with a solid to solvent ratio of 1:30 (w/v) (Liu et al, 2013) was used as solvent due to its wide application in the extraction of Schisandra lignans (Lee & Kim, 2010;Zhang et al, 2009).…”

“…Methanol, with a solid to solvent ratio of 1:30 (w/v) (Liu et al, 2013) was used as solvent due to its wide application in the extraction of Schisandra lignans (Lee & Kim, 2010;Zhang et al, 2009). Each sample (1 g) was extracted with 30 mL methanol at room temperature for 1 h in a sonication bath (Sonorex Super, Bandelin, electronic GmbH, Berlin, Germany).…”

“…1A presents a typical HPLC-DAD chromatogram of the fruits' extract recorded at 260 nm. The peaks were assigned by comparing their retention times, UV absorbance, and mass spectrometric data with commercially available reference compounds (when available), or were tentatively assigned by comparing their mass spectrometric data with established compound libraries and literature published (Liu et al, 2013). Precursor ions determined based on the m/z value of…”

A high throughput mass spectrometry screening analysis based on two-dimensional carbon microfiber fractionation system

“…Among them, only peaks with the same mass (10 µg mL -1 tolerance) of compounds already revealed in Schisandra Chinensis extracts, which were considered to confirm the presence of the relevant substance in the analyzed real samples [41][42][43][44][45][46][47][48][49][50]. Table 2 shows the results of this investigation.…”

“…The major active components of Schisandra Chinensis are lignans and flavonoids, which have been found to possess bioactive characteristics preventing breast, colon, prostate and thyroid cancer, hepatotoxicity and heart disease. Table 2 The results obtained with this new approach for Schisandra Chinensis extracts were compared with the ones achieved with chromatographic methods such as 2D/1D HPLC-MS [41,44,48,51] and direct MS [42,52] (see Table 3). Analysis time of 2DµCFs-ESI-MS can be considered comparable with direct MS, while the number of detected compounds is similar to that obtained with classical chromatographic methods.…”