Comprehensive characterization of the in vitro and in vivo metabolites of ziyuglycoside I in rat microsome, intestinal flora, excretion specimen and fresh tissues based on LC–Q-TOF/MS

Wang, Guangji; Fu, Hanxu; Ye, Wei; Zheng, Xiao; Xiao, Jingcheng; Kang, Dian; Rao, Tai; Shao, Yuhao; Xie, Lin; Liang, Yan

doi:10.1016/j.jpba.2016.05.032

Cited by 26 publications

(10 citation statements)

References 24 publications

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“…Although numerous UHPLC-tandem mass spectrometry (MS/MS) methods have been applied to the determination of bioactivities components in traditional Chinese medicines [19,20], few studies have applied this method to quantitative analysis of flavonoids in D. odorifera [15][16][17]21]. Additionally, UHPLC-quadrupole time-of-flight (Q/TOF)-MS/MS has become increasingly important in compound identification because of its high selectivity, specificity, and accuracy [22].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Flavonoids in Dalbergia odorifera by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry

et al. 2020

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Dalbergia odorifera, a traditional Chinese medicine, has been used to treat cardio- and cerebrovascular diseases in China for thousands of years. Flavonoids are major active compounds in D. odorifera. In this paper, a rapid and sensitive ultra-high performance liquid chromatography-triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 17 flavonoids in D. odorifera. Quantification was performed by multiple reaction monitoring using electrospray ionization in negative ion mode. Under the optimum conditions, calibration curves for the 17 analytes displayed good linearity (r2 > 0.9980). The intra- and inter-day precisions (relative standard deviations) were lower than 5.0%. The limit of quantitation ranged from 0.256 to 18.840 ng/mL. The mean recovery range at three spiked concentrations was 94.18–101.97%. The validated approach was successfully applied to 18 samples of D. odorifera. Large variation was observed for the contents of the 17 analytes. Sativanone and 3′-O-methylviolanone were the dominant compounds. The fragmentation behaviors of six flavonoids were investigated using UPLC with quadrupole time-of-flight tandem mass spectrometry. In negative ion electrospray ionization mass spectrometry, all the flavonoids yielded prominent [M − H]− ions. Fragments for losses of CH3, CO, and CO2 were observed in the mass spectra. Formononetin, liquiritigenin, isoliquiritigenin, sativanone, and alpinetin underwent retro-Diels–Alder fragmentations. The proposed method will be helpful for quality control of D. odorifera.

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Section: Introductionmentioning

confidence: 99%

Analysis of Flavonoids in Dalbergia odorifera by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry

et al. 2020

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“…Anaerobic culture medium was prepared as follows [ 21 ]: K 2 HPO 4 (37.5 mL, 0.78%), solution A (37.5 mL, 0.47% KH 2 PO 4 , 1.18% NaCl, 1.2% (NH 4 ) 2 SO 4 , 0.12% CaCl 2 , and 0.25% MgSO 4 •H 2 O), Na 2 CO 3 (25 mL, 8%), l -cysteine (0.5 g), l -ascorbic acid (2 mL, 25%), eurythrol (1 g), and tryptone (1 g) were mixed together and diluted with distilled water to 1 L. The solution was then adjusted to pH 7.5–8.0 with 2 M HCl. Fresh feces collected from Sprague-Dawley rats were immediately homogenized in 0.9% ( w / v ) NaCl aqueous solution at a ratio of 1 g to 4 mL.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the In Vivo and In Vitro Metabolites of Linarin in Rat Biosamples and Intestinal Flora Using Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

et al. 2018

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Linarin, a flavone glycoside, is considered to be a promising natural product due to its diverse pharmacological activities, including analgesic, antipyretic, anti-inflammatory and hepatoprotective activities. In this research, the metabolites of linarin in rat intestinal flora and biosamples were characterized using ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS). Three ring cleavage metabolites (4-hydroxybenzoic acid, 4-hydroxy benzaldehyde and phloroglucinol) were detected after linarin was incubated with rat intestinal flora. A total of 17 metabolites, including one ring cleavage metabolite (phloroglucinol), were identified in rat biosamples after oral administration of linarin. These results indicate that linarin was able to undergo ring fission metabolism in intestinal flora and that hydrolysis, demethylation, glucuronidation, sulfation, glycosylation, methylation and ring cleavage were the major metabolic pathways. This study provides scientific support for the understanding of the metabolism of linarin and contributes to the further development of linarin as a drug candidate.

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“…Anaerobic culture medium was prepared as follows: the solution A (0.78% K 2 HPO 4 ) 37.5 mL, the solution B (0.47% KH 2 PO 4 , 1.18% NaCl, 1.2% (NH4) 2 SO 4 , 0.12% CaCl 2 , 0.25% MgSO 4 ⋅H 2 O) 37.5 mL, L-cysteine 0.5 g, 25% L-ascorbic acid 2 mL, 8% Na 2 CO 3 50 mL, eurythrol 1 g, peptone 1 g, and nutrient agar 1 g were mixed together and diluted with distilled water to 1 L [9]. The pH was adjusted to 7.5-8.0 with HCl before autoclaving at 121 • C for 20 min.…”

Section: General Anaerobic Medium Broth Preparationmentioning

confidence: 99%

“…Therefore, it can be seen that intestinal bacteria plays an important role in the metabolism of TCM glycosides. Currently, intestinal bacteria have been widely used in the metabolism study in vitro of active ingredients in TCMs [9].…”

mentioning

confidence: 99%

Metabolites study on 5‐O‐methylvisammioside in vivo and in vitro by ultra high performance liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

Fan

Liu

Meng

et al. 2019

J of Separation Science

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5‐O‐Methylvisammioside is one of major chromones of Radix Saposhnikoviae possessing definite pharmacological activities, but there are few reports with respect to the metabolism of 5‐O‐methylvisammioside. In this work, metabolites in vivo were explored in male Sprague‐Dawley rats and in vitro investigated on rat intestinal bacteria incubation model and were identified by using ultra high performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry. An online data acquisition method based on a multiple mass defect filter and dynamic background subtraction was developed to trace all probable metabolites. As a result, 26 metabolites in vivo (including 18, 15, 10, and 10 in rat urine, faece, bile, and blood) and 7 metabolites in vitro were characterized, respectively. Additionally, the main metabolic pathways in vivo and in vitro, including deglycosylation, deglycosylation + demethylation, deglycosylation + oxidation, N‐acetylation, and sulfate conjugation, were summarized by calculating the relative content of each metabolite. The obtained results significantly enriched our knowledge about 5‐O‐methylvisammioside metabolism and will lead to a better understanding of its safety and efficacy.

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Comprehensive characterization of the in vitro and in vivo metabolites of ziyuglycoside I in rat microsome, intestinal flora, excretion specimen and fresh tissues based on LC–Q-TOF/MS

Cited by 26 publications

References 24 publications

Analysis of Flavonoids in Dalbergia odorifera by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry

Analysis of Flavonoids in Dalbergia odorifera by Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry

Characterization of the In Vivo and In Vitro Metabolites of Linarin in Rat Biosamples and Intestinal Flora Using Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

Metabolites study on 5‐O‐methylvisammioside in vivo and in vitro by ultra high performance liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry

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