Comprehensive characterization of nanostructured lipid carriers using laboratory and synchrotron X-ray scattering and diffraction

Tetyczka, Carolin; Hodžić, Aden; Kriechbaum, Manfred; Juraić, Krunoslav; Spirk, Christina; Hartl, Sonja; Pritz, Elisabeth; Leitinger, Gerd; Roblegg, Eva

doi:10.1016/j.ejpb.2019.03.017

Cited by 22 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the following scale based on the reduction of the crystallinity value can be described: L-NLC > R-NLC > M-NLC > N-NLC > CT-NLC. The lowest crystallinity found for caprylic/capric triglyceride is consistent with literature findings, in which the addition of a synthetic oil was demonstrated to strongly reduce the crystallinity of the lipid matrix [ 61 , 62 ]. It is interesting to highlight that the crystallinity scale presents the same order of the stability scale obtained by Turbiscan ® analysis, with L-NLC and R-NLC as the most stable formulations compared to other NLC.…”

Section: Resultssupporting

confidence: 90%

Essential Oil-Loaded NLC for Potential Intranasal Administration

et al. 2021

View full text Add to dashboard Cite

Complementary and alternative medicines represent an interesting field of research on which worldwide academics are focusing many efforts. In particular, the possibility to exploit pharmaceutical technology strategies, such as the nanoencapsulation, for the delivery of essential oils is emerging as a promising strategy not only in Italy but also all over the world. The aim of this work was the development of nanostructured lipid carriers (NLC) for the delivery of essential oils (Lavandula, Mentha, and Rosmarinus) by intranasal administration, an interesting topic in which Italian contributions have recently increased. Essential oil-loaded NLC, projected as a possible add-on strategy in the treatment of neurodegenerative diseases, were characterized in comparison to control formulations prepared with Tegosoft CT and Neem oil. Homogeneous (polydispersity index, PDI < 0.2) nanoparticles with a small size (<200 nm) and good stability were obtained. Morphological and physical-chemical studies showed the formation of different structures depending on the nature of the liquid oil component. In particular, NLC prepared with Lavandula or Rosmarinus showed the formation of a more ordered structure with higher cytocompatibility on two cell lines, murine and human fibroblasts. Taken together, our preliminary results show that optimized positively charged NLC containing Lavandula or Rosmarinus can be proposed as a potential add-on strategy in the treatment of neurodegenerative diseases through intranasal administration, due to the well-known beneficial effects of essential oils and the mucoadhesive properties of NLC.

show abstract

Section: Resultssupporting

confidence: 90%

Essential Oil-Loaded NLC for Potential Intranasal Administration

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This suggests that the solid lipid has a crystalline nature and it can be assumed that polymorph C is dominating (see Figure 2 ). ( Kelidari et al, 2017 ; Zoubari et al, 2017 ; Tetyczka et al, 2019 ; Kumar et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Investigation of Cellular Interactions of Lipid-Structured Nanoparticles With Oral Mucosal Epithelial Cells

Jeitler

Glader

Tetyczka

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

Lipid-based nanosystems enable intracellular delivery of drugs in the oral cavity for the treatment of local diseases. To rationally design such systems, suitable matrix compositions and particle properties need to be identified, and manufacturing technologies that allow reproducible production have to be applied. This is a prerequisite for the reliable and predictable performance of in-vitro biological studies. Here, we showed that solid lipid nanoparticles (SLN, palmitic acid) and nanostructured lipid carriers (NLC, palmitic acid and oleic acid in different ratios) with a size of 250 nm, a negative zeta potential, and a polydispersity index (PdI) of less than 0.3 can be reproducibly prepared by high-pressure homogenization using quality by design and a predictive model. SLN and NLC were colloidally stable after contact with physiological fluid and did not form agglomerates. The in-vitro studies clearly showed that besides particle size, surface charge and hydrophobicity, matrix composition had a significant effect. More specifically, the addition of the liquid lipid oleic acid increased the cellular uptake capacity without changing the underlying uptake mechanism. Regardless of the matrix composition, caveolin-mediated endocytosis was the major route of uptake, which was confirmed by particle localization in the endoplasmic reticulum. Thus, this work provides useful insights into the optimal composition of lipid carrier systems to enhance the intracellular uptake capacity of drugs into the oral mucosa.

show abstract

“…Oil red o labeled NLC revealed a z-average of 293.44 ± 19.72 nm with a narrow particle size distribution (i.e., PdI values of 0.241 ± 0.042). The zeta potential of blank NLC and labeled NLC was −37.27 ± 0.82 mV and −39.16 ± 4.09 mV, respectively, indicating good physical stability [ 34 ]. Moreover, the particles remained stable after dispersion in DMEM (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…Oil red o labeled NLC were prepared according to Tetyczka et al [ 33 , 34 ]. Briefly, oil red o (2% w / w ; % with regard to the lipid phase) was mixed with palmitic acid and oleic acid (10% w / w ; ratio 9:1 palmitic acid and oleic acid) and heated to 70 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Oil red o labeled NLC were prepared according to Tetyczka et al [33,34]. Briefly, oil red o (2% w/w; % with regard to the lipid phase) was mixed with palmitic acid and oleic acid (10% w/w; ratio 9:1 palmitic acid and oleic acid) and heated to 70 • C. Subsequently, an aqueous stabilizer solution (i.e., 2% w/w Tween 80) that was heated to the same temperature was added.…”

Section: Preparation Of Nanostructured Lipid Carriers Via High Pressure Homogenizationmentioning

confidence: 99%

See 1 more Smart Citation

Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers

et al. 2021

Self Cite

View full text Add to dashboard Cite

Topical drug administration to the oral mucosa proves to be a promising treatment alternative for inflammatory diseases. However, disease-related changes in the cell barrier must be considered when developing such delivery systems. This study aimed at investigating the changes in the lining mucosa caused by inflammation and evaluating the consequences on drug delivery systems such as nanostructured lipid carriers (NLC). For this, TR146 cells were treated with inflammatory cytokines and bacterial components. Cell viability and integrity, reactive oxygen species (ROS), and interleukin (IL)-8 release were used as endpoints to assess inflammation. Translocation of phosphatidylserine, cytoskeletal arrangement, opening of desmosomes, and cell proliferation were examined. Transport studies with NLC were performed considering active and passive pathways. The results showed that IL-1ß and tumor necrosis factor α induced inflammation by increasing IL-8 and ROS production (22-fold and 2-fold). Morphologically, loss of cell–cell connections and formation of stress fibers and hyperplasia were observed. The charge of the cell membrane shifted from neutral to negative, which increased the absorption of NLC due to the repulsive interactions between the hydrophobic negative particles and the cell membrane on the one hand, and interactions with lipophilic membrane proteins such as caveolin on the other.

show abstract

Comprehensive characterization of nanostructured lipid carriers using laboratory and synchrotron X-ray scattering and diffraction

Cited by 22 publications

References 42 publications

Essential Oil-Loaded NLC for Potential Intranasal Administration

Essential Oil-Loaded NLC for Potential Intranasal Administration

Investigation of Cellular Interactions of Lipid-Structured Nanoparticles With Oral Mucosal Epithelial Cells

Cytokine-Mediated Inflammation in the Oral Cavity and Its Effect on Lipid Nanocarriers

Contact Info

Product

Resources

About