Comprehensive characterization of multiple components and metabolites of Xiaojin Capsule based on ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

Sceptridium ternatum is a medicinal herb with multiple health benefits. However, its antioxidant activity and active components have not been clarified. In this study, the antioxidant capacity of S. ternatum was comprehensively investigated using multiple colorimetric methods and 1,1‐diphenyl‐2‐picrylhydrazyl–high‐performance liquid chromatography analysis. First, the phenolic content, flavonoid content, and radical scavenging ability of S. ternatum were parallelly determined using colorimetric methods performed in 96‐well microplates. The flavonoid content, rather than the phenolic content, was highly correlated with its antioxidant activity. Sceptridium ternatum was shown to be a rich source of flavonoids, with a highest flavonoid yield of 3.44 ± 0.11 mg/g. Subsequently, 1,1‐diphenyl‐2‐picrylhydrazyl–high‐performance liquid chromatography experiment and quadrupole time‐of‐flight mass spectrometry analyses were carried out for rapid screening of the individual antioxidants. A total of 14 O‐glycosyl flavonoids with quercetin or kaempferol aglycone have been characterized. Particularly, quercetin 3‐O‐rhamnoside‐7‐O‐glucoside exhibited the most potent antioxidant ability. Its half‐maximal effective concentrations for scavenging 1,1‐diphenyl‐2‐picrylhydrazyl and 2,2ʹ‐azino‐bis (3‐ethylbenzthiazoline‐6‐sulfonic acid) radicals were 70.55 ± 2.69 and 106.90 ± 1.76 µg/mL, respectively, which were comparable with those of l‐ascorbic acid. Our results indicated that the combined colorimetric and chromatographic methods provided a practical strategy for the discovery of bioactive compounds from natural products.

Section: Structural Identification Of the Compounds In Sceptridium Tementioning

confidence: 99%

Comprehensive evaluation of the antioxidant capacity of Sceptridium ternatum using multiple colorimetric methods and 1,1‐diphenyl‐2‐picrylhydrazyl–high‐performance liquid chromatography analysis

Zhu

Zhong

Kong

et al. 2020

“…Compared with other quantitative methods for 10-HTCP and TET, the internal standard method introduced in our study could greatly reduce the system error of instrument determination process. The technique of UHPLC-ESI-MS/MS is widely implemented to study the drugs in vivo [25][26][27][28]. To the best of our current knowledge, the pharmacokinetic of 10-HCPT-TET complexes liposome has not been reported yet.…”

Section: Introductionmentioning

confidence: 97%

“…The technique of UHPLC-ESI-MS/MS is widely implemented to study the drugs in vivo [25][26][27][28]. Alkaline separation conditions provide improved peak shape for TET, with less tailing.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics of 10‐hydroxycamptothecin–tetrandrine liposome complexes in rat by a simple and sensitive ultra‐high performance liquid chromatography with tandem mass spectrometry

Shuang

Wang

Mao

et al. 2019

10‐Hydroxycamptothecin is a drug to cure various cancers. However, the 10‐hydroxycamptothecin cannot be widely applied in clinics due to fast elimination and resistance of various cancers to the drug. Nevertheless, co‐treatment with tetrandine is known to reverse the resistance of multi‐drug resistant cancers, and may present an effective strategy to improve the efficacy of 10‐hydroxycamptothecin. In order to improve the bioavailability and prolong the treatment time of the 10‐hydroxycamptothecin in vivo, we prepared 10‐hydroxycamptothecin‐tetrandrine liposome complexes with 10‐hydroxycamptothecin as the basic anticancer drug, tetrandrine and liposomes as carriers. In this article, an ultra‐high performance liquid chromatography tandem mass spectrometry method for the analysis of 10‐hydroxycamptothecin and tetrandrine in plasma has been developed, validated, and utilized to compare the pharmacokinetics of both drugs in the original dosage form and administered as liposome complexes. According to the pharmacokinetic parameters of mean residence time, half‐life period and clearance rate, the 10‐hydroxycamptothecin‐tetrandrine liposome complexes prolongs the retention and circulation time of 10‐hydroxycamptothecin in vivo, achieving a good sustained release effect. To the best of our current knowledge, the pharmacokinetic properties of 10‐hydroxycamptothecin‐tetrandrine liposome complexes in rats have not been reported yet. Our study can provide a helpful reference for further related study.

“…LC‐MS/MS is widely regarded as an excellent tool for non‐target identification and characterization of the ingredients in complex TCMs [6–16]. In a previous article, 87 main components in QFPD were characterized using UHPLC‐LTQ‐Orbitrap‐MS/MS [17].…”

Section: Introductionmentioning

confidence: 99%

Chemical profiling of Qingfei Paidu Decoction by triplex off‐line two‐dimensional liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry

Shen

Zhou

Xiong

et al. 2022

Qingfei Paidu Decoction is a Chinese medicine formula that has been proved effective in the treatment of coronavirus disease 2019. However, the comprehensive separation and characterization of Qingfei Paidu Decoction are of a great challenge due to the diversity of chemical components in a wide range of polarity. In this study, a triplex off‐line two‐dimensional liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry is developed for the analysis of Qingfei Paidu Decoction. One reversed‐phase liquid chromatography×hydrophilic interaction liquid chromatography system and two reversed‐phase liquid chromatography×reversed phase liquid chromatography systems were constructed to separate polar components and weak‐polar components in Qingfei Paidu Decoction, respectively. Benefiting from the good orthogonality of two‐dimensional liquid chromatography and high sensitivity of quadrupole time‐of‐flight MS, chemical components with different polarities and content were discovered. A total of 749 peaks were detected in positive and negative ionization mode and presented as a four‐dimensional data plot. Meanwhile, 498 compounds belonging to 14 categories were tentatively identified. These results provide good supplementary to elucidate the material basis of Qingfei Paidu Decoction. The triplex off‐line two‐dimensional liquid chromatography‐quadrupole time‐of‐flight mass spectrometry strategy can be a powerful and efficient tool for the separation and characterization of chemical substances in traditional Chinese medicine formulas.