Comprehensive characterization of in vivo metabolic profile of Polygalae radix based on ultra-high-performance liquid chromatography–tandem mass spectrometry

Feng, Guifang; Liu, Shu; Pi, Zifeng; Song, Fengrui; Liu, Zhiqiang

doi:10.1016/j.jpba.2018.12.005

Cited by 13 publications

(6 citation statements)

References 28 publications

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“…In our future report, we aim to show the multi-ion channel mechanism of DFD. In addition, with the development of mass spectrometry, multiple constituents of the components of DFD have been analyzed and characterized in recent years [29][30][31][32][33][34]. Modern pharmacological studies have shown that the components of DFD have anti-inflammatory [35][36][37][38][39], antioxidative stress [40][41][42], and myocardial protection [42][43][44][45] effects.…”

Section: Discussionmentioning

confidence: 99%

Antiarrhythmic Mechanisms of Chinese Herbal Medicine Dingji Fumai Decoction

Liang

Zhou

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Dingji Fumai decoction (DFD) is used to treat ventricular arrhythmia, and it has provided a very good curative effect. However, its cellular electrophysiological mechanism is unknown. Methods. Electrocardiogram was recorded, and oxidative stress response and ion-channel-related molecules were detected in rats with barium chloride- and aconitine-induced ventricular arrhythmia. Moreover, whole-cell patch-clamp assay was used to investigate the inhibitory effect of DFD on Nav1.5 in Chinese hamster ovary cells. Results. DFD prolonged the occurrence time and shortened the duration of ventricular arrhythmia, decreased the malondialdehyde and increased the superoxide dismutase, and alleviated the activation of Na+-K+-ATPase and connexin-43. DFD suppressed Nav1.5dose-dependently with an IC50 of 24.0 ± 2.4 mg/mL. Conclusions. The clinical antiarrhythmic mechanisms of DFD are based on its antioxidant potential, alleviation of Na+-K+-ATPase and connexin-43, and class I antiarrhythmic properties by suppressing Nav1.5dose-dependently with an IC50 of 24.0 ± 2.4 mg/mL.

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Section: Discussionmentioning

confidence: 99%

Antiarrhythmic Mechanisms of Chinese Herbal Medicine Dingji Fumai Decoction

Liang

Zhou

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…The results differ-depending on the structure of the studied compounds. In the studies of Feng et al [87], the saponins from Polygalae radix, which belong to the group of triterpene saponins, e.g., desacylsenegasaponin B, were found to not be able to penetrate the BBB. None from the seven saponins' metabolites present in the plasma were detected in the brains of rats.…”

Section: In Vivo and Postmortem Studiesmentioning

confidence: 99%

In Silico Studies on Triterpenoid Saponins Permeation through the Blood–Brain Barrier Combined with Postmortem Research on the Brain Tissues of Mice Affected by Astragaloside IV Administration

Stępnik

Kukuła-Koch

2020

IJMS

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As the number of central nervous system (CNS) drug candidates is constantly growing, there is a strong need for precise a priori prediction of whether an administered compound is able to cross the blood–brain barrier (BBB). The aim of this study was to evaluate the ability to cross the BBB of triterpenoid saponins occurring in Astragalus mongholicus roots. The research was carried out using in silico methods combined with postmortem studies on the brain tissues of mice treated with isolated astragaloside IV (AIV). Firstly, to estimate the ability to cross the BBB by the tested saponins, new quantitative structure–activity relationship (QSAR) models were established. The reliability and predictability of the model based on the values of the blood–brain barrier penetration descriptor (logBB), the difference between the n-octanol/water and cyclohexane/water logP (ΔlogP), the logarithm of n-octanol/water partition coefficient (logPow), and the excess molar refraction (E) were both confirmed using the applicability domain (AD). The critical leverage value h* was found to be 0.128. The relationships between the standardized residuals and the leverages were investigated here. The application of an in vitro acetylcholinesterase-inhibition test showed that AIV can be recognized as the strongest inhibitor among the tested compounds. Therefore, it was isolated for the postmortem studies on brain tissues and blood using semi-preparative HPLC with the mobile phase composed of water, methanol, and ethyl acetate (1.7:2.1:16.2 v/v/v). The results of the postmortem studies on the brain tissues show a regular dependence of the final concentration of AIV in the analyzed brain samples of animals treated with 12.5 and 25 mg/kg b.w. of AIV (0.00012299 and 0.0002306 mg, respectively, per one brain). Moreover, the AIV logBB value was experimentally determined and found to be equal to 0.49 ± 0.03.

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“…In rats, 29 triterpene metabolites were identified in feces after the administration of an ethanolic P. tenuifolia root extract, indicating that these metabolites are not absorbed in vivo but can have local effects on the intestinal microbiome. The altered microbiome may, in turn, indirectly affect brain function through the MGBA [ 160 ].…”

Section: Resultsmentioning

confidence: 99%

Medicinal Plants and Their Impact on the Gut Microbiome in Mental Health: A Systematic Review

et al. 2022

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Background: Various neurocognitive and mental health-related conditions have been associated with the gut microbiome, implicating a microbiome–gut–brain axis (MGBA). The aim of this systematic review was to identify, categorize, and review clinical evidence supporting medicinal plants for the treatment of mental disorders and studies on their interactions with the gut microbiota. Methods: This review included medicinal plants for which clinical studies on depression, sleeping disorders, anxiety, or cognitive dysfunction as well as scientific evidence of interaction with the gut microbiome were available. The studies were reported using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Results: Eighty-five studies met the inclusion criteria and covered thirty mental health-related medicinal plants with data on interaction with the gut microbiome. Conclusion: Only a few studies have been specifically designed to assess how herbal preparations affect MGBA-related targets or pathways. However, many studies provide hints of a possible interaction with the MGBA, such as an increased abundance of health-beneficial microorganisms, anti-inflammatory effects, or MGBA-related pathway effects by gut microbial metabolites. Data for Panax ginseng, Schisandra chinensis, and Salvia rosmarinus indicate that the interaction of their constituents with the gut microbiota could mediate mental health benefits. Studies specifically assessing the effects on MGBA-related pathways are still required for most medicinal plants.

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Comprehensive characterization of in vivo metabolic profile of Polygalae radix based on ultra-high-performance liquid chromatography–tandem mass spectrometry

Cited by 13 publications

References 28 publications

Antiarrhythmic Mechanisms of Chinese Herbal Medicine Dingji Fumai Decoction

Antiarrhythmic Mechanisms of Chinese Herbal Medicine Dingji Fumai Decoction

In Silico Studies on Triterpenoid Saponins Permeation through the Blood–Brain Barrier Combined with Postmortem Research on the Brain Tissues of Mice Affected by Astragaloside IV Administration

Medicinal Plants and Their Impact on the Gut Microbiome in Mental Health: A Systematic Review

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