Comprehensive characterization of cultivated in vitro Deschampsia antarctica E. Desv. plants with different chromosome numbers

Navrotska, D. O.; Андреев, И. О.; Parnikoza, Іvan; Spiridonova, K. V.; Poronnik, O.; Miryuta, N. Yu.; Myryuta, G. Yu.; Zahrychuk, O. M.; Дробык, Н. М.; Кунах, В. А.

doi:10.3103/s009545271706010x

Cited by 5 publications

(13 citation statements)

References 19 publications

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“…The presence of mixoploid plants in D. antarctica populations is maintained by its capacity of vegetative and apomictic propagation common in populations from the range limit, where environmental stress conditions prevail (e.g., Amosova et al, 2015). However, as it was shown by Navrotska et al (2017), genetic differentiation between D. antarctica individuals with anomalous karyotype and diploids did not differ significantly from genetic differentiation between individuals representing typical diploid plants. Therefore, more detailed cytological and molecular studies with extensive sampling are needed to check whether polyploidization in D. antarctica is common or rather rare event, and to test how the change in ploidy level may affect the genetic diversity of the species.…”

Section: Ta B L E 7 Estimates Of Mismatch Analysis Of Nine Populations Of Deschampsia Antarcticamentioning

confidence: 92%

“…Research conducted in marine Antarctica has shown that diploid individuals of D. antarctica predominate in the populations of this area. However, especially in the Southernmost populations the hypotriploid individuals and genotypes that had the B chromosome were also reported (Amosova et al, 2015;Navrotska et al, 2017). The presence of mixoploid plants in D. antarctica populations is maintained by its capacity of vegetative and apomictic propagation common in populations from the range limit, where environmental stress conditions prevail (e.g., Amosova et al, 2015).…”

Section: Ta B L E 7 Estimates Of Mismatch Analysis Of Nine Populations Of Deschampsia Antarcticamentioning

confidence: 99%

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Retrotransposon‐based genetic diversity of Deschampsia antarctica Desv. from King George Island (Maritime Antarctic)

Androsiuk

Chwedorzewska

Dulska

et al. 2020

Ecology and Evolution

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Section: Ta B L E 7 Estimates Of Mismatch Analysis Of Nine Populations Of Deschampsia Antarcticamentioning

confidence: 92%

Section: Ta B L E 7 Estimates Of Mismatch Analysis Of Nine Populations Of Deschampsia Antarcticamentioning

confidence: 99%

Retrotransposon‐based genetic diversity of Deschampsia antarctica Desv. from King George Island (Maritime Antarctic)

Androsiuk

Chwedorzewska

Dulska

et al. 2020

Ecology and Evolution

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“…Pr ik data sets were obtained by methods described in (Miryuta et al, 2016;; Scion Image http://scion-image.software.informer.com/ 4.0/); Glg i were data on genetic distances. Data were taken from the article (Navrotska et al, 2017) and obtained according to Jacquard method between plants of different D. antarctica genotypes, calculated by the results of ISSR and IRAP analysis (Navrotska et al, 2017), i = 4, 5, ... n (n = 11); Pg ij were the content of photosynthetic pigments, where j = 1, 2, 3 where pigment number corresponds to j = 1 is chlorophyll A (ChlA), j = 2 is chlorophyll B (ChlB), j = 3 is carotenoids (Car) (Poronnik et al, 2019). The data sets for Gs i , Fl i , and Glg i are shown in Tables 1 and 2 correspondingly, for Ph i , Pr ik , Pg ij , are shown in Figures 1-3.…”

Section: Methodsmentioning

confidence: 99%

“…Variability of plants based on molecular markers was studied by PCR analysis with ISSR and IRAP primers. In total, 63 amplicons were obtained for the investigated samples, among which 22 (34.2%) were polymorphic (Table 2) (Navrotska et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

“…But there is another task, to follow how the longterm conditions in different regions have influenced their accumulated in nature adaptive capacity to pre servation. This is more interesting because genetic studies of this species have shown a fairly low genetic heterogeneity (Andreev et al, 2010;Navrotska et al, 2017). The results of our previous studies of individual D. antarctica plants' adaptability of different origins are presented in (Miryuta et al, 2016;Parnikoza et al, 2017).…”

Section: Introductionmentioning

confidence: 95%

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Calculation of United Quality Latent Indices of Deschampsia antarctica plants adaptability of different origin grown in vitro

Miryuta

Parnikoza

Poronnik

et al. 2021

UAJ

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The research was to develop and describe in detail the algorithm for calculating the United Quality Latent Index (UQLI, Iq ) of plant adaptability from the collection of Deschampsia antarctica Ė. Desv. genotypes obtained from seeds collected at different sites in the Argentine Islands region, the maritime Antarctic, and grown in vitro at the laboratory conditions. Genome size and genetic distances by ISSR and IRAP markers according to data from published articles were used as basic indices of initial genetic heterogeneity for analyzed plant genotypes. To assess individual adaptability indices for eleven D. antarctica genotypes, we used measurement of the leaf length morphometric index and determination of the flavonoids content by rutin and the content of photosynthetic pigments. The spectra of reserve and protective proteins in leaves were investigated by polyacrylamide gel electrophoresis. To obtain the United Quality Latent Index of Adaptability (Iqi, UQLI), the method of extreme grouping was used. The estimation of Iqi (UQLI) was performed using pairwise comparisons of indices from differences sets for each pair of genotypes. We developed and described in detail the algorithm for Iqi estimation for eleven D. antarctica genotypes. As an example of application, correlation models of probability relations of the indices are presented. To evaluate the complex adaptability for eleven D. antarctica genotypes grown in vitro we used developed algorithm for the UQLI calculation. The individuality of the adaptive portrait for all studied genotypes under in vitro cultivation conditions was shown. The influence of basic genetic characteristics (genome size and genetic distances) on auxin metabolism-related indices of leaf length and flavonoid content was shown. Such effect may be carried out by genetic characteristics both individually and together, probably via auxin metabolism. Among the eight genotypes researched, we distinguish four different variants by correlation models and two (positive and negative) by the general Iqi value. Thus the Iqi (UQLI) is proposed to describe a large number of source data at different organization levels which characterize sample genotypes by reducing the dimensions to one dimensionless number. This genotypes’ individuality and the peculiarities of their grouping by Iqi should be taken into account when doing experimental studies using these genotypes as model plants, especially in experiments studying the regulation of productivity and the effect of the various exogenous factors, etc.

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Polish Polar Research

Nuzhyna,

Parnikoza,

Poronnik

et al. 2019

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This paper presents a comparative study on the anatomy of the Antarctic hairgrass (Deschampsia antarctica É. Desv.) from natural populations of two distant maritime Antarctic regions: the Argentine Islands (Antarctic Peninsula region) and the Point Thomas oasis (King George Island, South Shetland Islands). Comparison of D. antarctica plants from natural populations of Argentine Islands region and plants originated from seeds of these populations cultivated in vitro also was made. Additionally anatomical features of Deschampsia antarctica were compared with ones for D. caespitosa. The results of our study do not provide enough evidence to assert more pronounced xerophytic anatomical features in D. antarctica plants from more harsh conditions of Argentine Islands region. Such features (both qualitative and quantitative) of D. antarctica mainly depend on local conditions, and not on the latitudinal or climatic gradient. In both regions it is possible to find individuals that represent different ecotypes which are adopted to open arid or more humid habitats. It has been shown that Antarctic hairgrass plants germinated from seeds and cultivated in vitro retain the qualitative anatomy features that are typical to plants from the initial natural populations. This is especially noticeable in the case of plants from Berthelot Island (BE1 study plots), which might indicate a genetic fixation and a manifested differentiation similar to DNA haplotypes or chromosomal forms. However, quantitative characteristics, in particular the epidermis parameters, are subject to changes due to the transfer to more favourable conditions. Also qualitative and quantitative difference of D. antarctica in contrast with D. caespitosa have been described. These differences could be useful for identifying these two species. Additionally the quantitative differences (such as the area of the epidermal cells and the number and size of stomata on the adaxial surface) of Alaskan D. caespitosa grown from seeds were detected in contrast to the naturally grown plants of the same species from Ushuaia.

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Comprehensive characterization of cultivated in vitro Deschampsia antarctica E. Desv. plants with different chromosome numbers

Cited by 5 publications

References 19 publications

Retrotransposon‐based genetic diversity of Deschampsia antarctica Desv. from King George Island (Maritime Antarctic)

Retrotransposon‐based genetic diversity of Deschampsia antarctica Desv. from King George Island (Maritime Antarctic)

Calculation of United Quality Latent Indices of Deschampsia antarctica plants adaptability of different origin grown in vitro

Polish Polar Research

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