Comprehensive catecholaminergic projectome analysis reveals single-neuron integration of zebrafish ascending and descending dopaminergic systems

Abstract

Essential components of animal behaviour are modulated by dopaminergic (DA) and noradrenergic circuitry. In this study, we reveal at cellular resolution the complete set of projections ('projectome') of every single type of DA and noradrenergio neurons in the central nervous system of zebrafish larvae. The most extensive DA projections are established by posterior tubercular otp-dependent neurons, with individual somata integrating the ascending DA system, the descending diencephalospinal, as well as the endohypothalamic circuitry. These findings suggest a major role in the modulation of physiology and behaviour for otp-dependent DA neurons, which correlate with the mammalian A11 group. We further identified an endogenous subpallial DA system that not only provides most of the local DA projections, but also connects to the ventral diencephalon. The catecholaminergic projectome map provides a framework to understand the evolution and function of these neuromodulatory systems.

The developmental neurotoxicity of polybrominated diphenyl ethers: Effect of DE‐71 on dopamine in zebrafish larvae

“…All rights reserved present anterior to the midbrain-hindbrain boundary in zebrafish [22]. With this in mind, we confirmed our TH western blot expression data by performing whole-mount immunofluorescence assay, as previously described [23].…”

Olfactory projectome in the zebrafish forebrain revealed by genetic single-neuron labelling

“…We find that the axons and their synaptic terminals of OB output neurons are closely associated with processes of dopaminergic neurons in the PT, rendering them a strong candidate for postsynaptic partner. Because a population of dopaminergic neurons in the PT send descending axons directly to the spinal cord 37 , it is conceivable that the OB-PT pathway may play general roles in olfaction such as evoking locomotion 47,48 . Further studies are required to precisely identify types of PT neurons connecting with the OB output neurons and to elucidate the functional aspects of this unique neural circuitry.…”

“…The PT is a hypothalamus-related nucleus, containing populations of dopaminergic neurons that project to distant brain regions 37,38 . A large proportion of tbx21 tg þ neurons from every glomerular cluster (63-100%) and a smaller proportion (33%) of lhx2a tg þ neurons from mdG were observed to send a long axon to the PT (Fig.…”

Amigo Adhesion Protein Regulates Development of Neural Circuits in Zebrafish Brain

“…The vcc and the hindbrain cell cluster are important for the development of early ascending and descending long tracts (35,36) and were found to be correctly located in the knockdown morphants (Fig. 6A, vcc and hc).…”