Comprehensive bioinformatics and experimental analysis of SH3PXD2B reveals its carcinogenic effect in gastric carcinoma

Zhu, Ying; Hu, Yunhong; Wang, Peipei; Dai, Xinyang; Fu, Yuhan; Xia, Yuwei; Sun, Leitao; Ruan, Shanming

doi:10.1016/j.lfs.2023.121792

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…After constructing the network, we further analyzed the immune infiltration related to ENO2 and IGF2BP3 in CRC, observing a connection between gene expression and immune cell infiltration. Although previous studies have reported that the behavior and prognosis of tumor patients may be influenced by immune infiltration (Zhu et al 2023 ), the interaction mechanism between ENO2, IGF2BP3, and the tumor microenvironment (TME) in CRC remains unclear. Our analysis results indicated that the expression of ENO2 and IGF2BP3 was positively correlated with neutrophil, Tregs, T cell follicular helper, macrophage M0, and macrophage M1; while it was negatively correlated with macrophage M2.…”

Section: Discussionmentioning

confidence: 99%

Effects of hsa_circ_0074854 on colorectal cancer progression, construction of a circRNA–miRNA–mRNA network, and analysis of immune infiltration

Fang,

Xu,

Zhang

et al. 2023

J Cancer Res Clin Oncol

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Purpose Circular RNAs have been demonstrated to be closely associated with the onset and metastasis of colorectal cancer. However, the roles and clinical diagnostic value of most circRNAs in colorectal cancer remain unclear. Methods We detected the differential expression of circRNAs in CRC tissues and cells and investigated their relationship in conjunction with clinical pathological features. Additionally, we performed cellular functional experiments in CRC cell lines to explore the functions of circRNAs. To further validate the potential ceRNA network, qPCR was performed to assess the expression of miRNA and mRNA in CRC cells after differential expression of circRNAs knockdown. Furthermore, database analysis was utilized to explore the relationship between the predicted mRNAs and immune infiltration in CRC. Results Our research findings indicate a positive correlation between hsa_circ_0074854 expression and advanced clinical pathological features, as well as an unfavorable prognosis. Knockdown of hsa_circ_0074854 was observed to inhibit proliferation and migration capabilities of colorectal cancer cells, affecting the cell cycle progression, and simultaneously promoting apoptosis. A competing endogenous RNA mechanism may exist among circRNAs, miRNAs, and mRNAs. Furthermore, the expression of target genes displayed correlations with the abundance of certain immune cells. Conclusion We propose a novel ceRNA network and evaluate the interplay between target genes and immune cells, providing novel insights for the diagnosis and targeted therapy of CRC.

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Section: Discussionmentioning

confidence: 99%

Effects of hsa_circ_0074854 on colorectal cancer progression, construction of a circRNA–miRNA–mRNA network, and analysis of immune infiltration

Fang,

Xu,

Zhang

et al. 2023

J Cancer Res Clin Oncol

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“…To analyze small-molecule drugs targeting DHODH, the GSCALite online database 31 was used to calculate the Spearman correlation coefficient to determine the correlation between DHODH expression levels and the drug sensitivity (50% inhibitory concentration (IC50)) of 65 small-molecule drugs from the GDSC and CTRP databases.…”

Section: Methodsmentioning

confidence: 99%

Bioinformatics analysis and experimental verification of the cancer-promoting effect of DHODH in clear cell renal cell carcinoma

Wang,

Li,

Lin

et al. 2024

Sci Rep

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Clear cell renal cell carcinoma (ccRCC) is a malignant tumor of the urinary system. To explore the potential mechanisms of DHODH in ccRCC, we analyzed its molecular characteristics using public databases. TCGA pan-cancer dataset was used to analyze DHODH expression in different cancer types and TCGA ccRCC dataset was used to assess differential expression, prognosis correlation, immune infiltration, single-gene, and functional enrichment due to DHODH. The GSCALite and CellMiner databases were employed to explore drugs and perform molecular docking analysis with DHODH. Protein–protein interaction networks and ceRNA regulatory networks of DHODH were constructed using multiple databases. The effect of DHODH on ccRCC was confirmed in vitro. DHODH was highly expressed in ccRCC. Immune infiltration analysis revealed that DHODH may be involved in regulating the infiltration of immunosuppressive cells such as Tregs. Notably, DHODH influenced ccRCC progression by forming regulatory networks with molecules, such as hsa-miR-26b-5p and UMPS and significantly enhanced the malignant characteristics of ccRCC cells. Several drugs, such as lapatinib, silmitasertib, itraconazole, and dasatinib, were sensitive to DHODH expression and exhibited strong molecular binding with it. Thus, DHODH may promote ccRCC progression and is a candidate effective therapeutic target for ccRCC.

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Surface electric field enhanced biosensor based on symmetrical U-tapered HCF structure for gastric carcinoma biomarker trace detection

Li,

Wang

2024

Biosensors and Bioelectronics

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Comprehensive bioinformatics and experimental analysis of SH3PXD2B reveals its carcinogenic effect in gastric carcinoma

Cited by 4 publications

References 40 publications

Effects of hsa_circ_0074854 on colorectal cancer progression, construction of a circRNA–miRNA–mRNA network, and analysis of immune infiltration

Effects of hsa_circ_0074854 on colorectal cancer progression, construction of a circRNA–miRNA–mRNA network, and analysis of immune infiltration

Bioinformatics analysis and experimental verification of the cancer-promoting effect of DHODH in clear cell renal cell carcinoma

Surface electric field enhanced biosensor based on symmetrical U-tapered HCF structure for gastric carcinoma biomarker trace detection

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