Comprehensive bioinformatics analysis reveals the prognostic value, predictive value, and immunological roles of ANLN in human cancers

Abstract

Anillin (ANLN) is a unique scaffolding, actin-binding protein, which is essential for the integrity and ingression of the cleavage furrow. It is mainly involved in the cytokinesis process, while its role in various tumors has not been fully addressed and remains largely elusive. To provide a thorough perspective of ANLN’s roles among diverse malignancies, we conducted a comprehensive, pan-cancer analysis about ANLN, including but not limited to gene expression levels, prognostic value, biological functions, interacting proteins, immune-related analysis, and predictive value. As a result, when compared to normal tissues, ANLN expression is elevated in most cancers, and its expression also differs in different immune subtypes and molecular subtypes in diverse cancers. In addition, in 17 types of cancer, ANLN expression is increased in early tumor stages, and higher ANLN expression predicts worse survival outcomes in more than ten cancers. Furthermore, ANLN shows close correlations with the infiltration levels of most immune cells, and enrichment analysis using ANLN co-expressed genes reveals that ANLN plays essential roles in cell cycle, mitosis, cellular senescence, and p53 signaling pathways. In the final, ANLN exhibits high accuracy in predicting many cancers, and subsequent multivariate analysis suggests ANLN could be an independent prognostic factor in specific cancer types. Taken together, ANLN is proved to be a novel and promising biomarker for its excellent predictive utility, promising prognostic value, and potential immunological roles in pan-cancer. Targeting ANLN might be an attractive approach to tumor treatment.

Transcriptome analysis of beta-catenin-related genes in CD34+ haematopoietic stem and progenitor cells from patients with AML

“…Another of our hub genes, Anillin (ANLN) , which is essential for cytokinesis, is elevated in the early stages of 17 cancers, and in more than ten tumors, higher expression of ANLN is associated with a poorer prognosis. 62 In our study, we found for the first time that AML patients with high levels of beta-catenin had higher expression of the ANLN gene.…”

Characterization of the prognostic, diagnostic, and immunological roles of DSCC1 and its genomic alteration and instability in human cancers

“…comp-genomics.org/) provides free access to explore gene expression and tumor immune infiltration in TCGA cohorts. Referring to previous research, we assessed the correlation of DSCC1 expression with myeloid-derived suppressor cells (MDSCs) and natural killer (NK) T cells (NKT/T cell NK) infiltration levels under the "immune" module [10]. Additionally, the single-sample GSEA algorithm, with gene sets consisting of markers of individual immune cells, enabled us to estimate the correlation of DSCC1 with the infiltration of 24 types of tumor-infiltrating lymphocytes (TILs) [11].…”