Comprehensive analysis of the complete mitochondrial genome of Melanoplus differentialis (Acrididae: Melanoplinae) captured in Korea

Abstract

In this study, we determined the complete mitochondrial genome of the invasive insect species Melanoplus differentialis captured in Korea. The complete mitochondrial genome of M. differentialis is 15,625 bp long and comprises 13 protein‐coding genes, two ribosomal RNA genes, and 22 transfer RNAs, with a GC ratio of 25.2%. In total, 353 SNPs and 11 INDEL regions (total length 67 bp) were found against the previously sequenced M. differentialis mitochondrial genome recorded as public genome data. The number of interspecific variations was greater than the number of intraspecific variations in this insect. Phylogenetic tree analysis showed that the mitochondrial genome clustered the Melanoplus clade with two previously reported Melanoplus sequences. However, the sequences were not divided at the species‐level clade possibly as a consequence of misidentification caused by an error in the public database. Our results extend the molecular database status of Melanoplus by providing a novel complete mitochondrial genome sequence for M. differentialis that could serve as reference for further molecular studies.

Comparative analyses of <i>Agrimonia</i> complete chloroplast genomes with newly assembled chloroplast genomes of <i>A. coreana</i> and <i>A. nipponica</i> (Rosaceae)

“…SSRs identified from ten Agrimonia chloroplast genomes were compared based on their flanking sequences under the SSRDB environment (http://ssrdb.infoboss.co.kr/) (Park et al, in preparation). The pipeline of the SSR comparison implemented in the SSRDB relies on various organelle genome studies (Park et al, 2021i, 2022bKim et al, 2023) and was used with the following conditions: a cut-off e-value of 1e-10 and a maximum flanking sequence for the comparison of 60 bp.…”