Comprehensive analysis of PTEN‐related ceRNA network revealing the key pathways WDFY3‐AS2 ‐ miR‐21‐5p/miR‐221‐3p/miR‐222‐3p ‐ TIMP3 as potential biomarker in tumorigenesis and prognosis of kidney renal clear cell carcinoma

Abstract

Kidney renal clear cell carcinoma (KIRC) is one of the most common malignancies, and there is still a lack of effective biomarkers for early detection and prognostic prediction.In here, we compared the characteristics of RNA sequencing data sets of KIRC samples based on the tumor suppressor gene phosphatase and tensin homolog (PTEN). The 1016 long noncoding RNAs, 48 microRNAs (miRNAs), and 2104 messenger RNAs associated with PTEN were identified and these genes were differentially expressed between tumor and paracancerous tissues. The most relevant pathway was found to be WDFY3-AS2 -miR-21-5p/miR-221-3p/miR-222-3p -TIMP3 according to the rules of competing endogenous RNA (ceRNA) regulation. WDFY3-AS2 and TIMP3 expression were positively correlated and reduced in KIRC samples, while miR-21-5p, miR-221-3p, and miR-222-3p were relatively highly expressed. The relatively low expression of WDFY3-AS2 and TIMP3 in KIRC were associated with poor prognosis in KIRC patients, while higher expression of miR-21-5p, miR-221-3p, and miR-222-3p predicted reduced survival (p < 0.05). Univariate and multivariate Cox regression analysis showed that lower expression of WDFY3-AS2 and TIMP3 was significantly related to tumor grade, tumor size, lymph node metastasis, distant metastasis, and TNM stage. The expression of TIMP3 in KIRC tissues was also verified by immunohistochemistry, and the results were consistent with our analytical data. In summary, this study constructed a new model with clinical predictive value and identified the WDFY3-AS2/TIMP3 pathway

Construction of CDKN2A-related competitive endogenous RNA network and identification of GAS5 as a prognostic indicator for hepatocellular carcinoma

“…, PTEN pseudogene, VAPA, BCL11B, etc. ), thus leading to the development of lung adenocarcinoma[ 30 ], renal clear cell carcinoma[ 31 ], and glioma[ 32 ]. Nevertheless, the predictive significance of ceRNA for HCC remains incompletely understood, and scant research has pinpointed ceRNA regulatory networks associated with CDKN2A that influence patient survival in HCC patients.…”

Construction of a Risk Model and Prediction of prognosis and immunotherapy Based on Cuproptosis-Related LncRNAs in the Urinary System Pan-Cancer

“…BDNF−AS may be a prognosis biomarker and inhibits the proliferation, invasion, migration, and EMT progressionlecular intervening target in prostate cancer [26] . lncRNA WDFY3-AS2 acts as a ceRNA to enhance TIMP3 expression by acting as a sponge for Mir-21-5p, and Mir-221-3p [27] . AC106820.5 is also a CRLs and prognosis biomarker in head and neck squamous cell carcinoma [28] .…”

Construction of a risk model and prediction of prognosis and immunotherapy based on cuproptosis-related LncRNAs in the urinary system pan-cancer

“…BDNF − AS may be a prognosis biomarker and inhibits the proliferation, invasion, migration, and EMT progression molecular intervening target in prostate cancer [ 27 ]. lncRNA WDFY3-AS2 acts as a ceRNA to enhance TIMP3 expression by acting as a sponge for Mir-21-5p, and Mir-221-3p [ 28 ]. AC106820.5 is also a CRLs and prognosis biomarker in head and neck squamous cell carcinoma [ 29 ].…”