Comprehensive analysis of phenolics compounds in citrus fruits peels by UPLC-PDA and UPLC-Q/TOF MS using a fused-core column

Sanches, Vitor L.; Cunha, Tanize A.; Viganó, Juliane; Mesquita, Leonardo M. de Souza; Faccioli, Lúcia Helena; Breitkreitz, Márcia Cristina; Rostagno, Maurı́cio A.

doi:10.1016/j.fochx.2022.100262

Cited by 30 publications

(17 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sanches et al . (2022) conducted a comprehensive analysis of the chemical composition of three citrus peels, namely lime (Citrus × latifolia), lemon (Citrus limon), and rangpur lime (Citrus × limonia), and identified twenty‐four polyphenols and five limonoids. As citrus peels are predominantly composed of polyphenols containing one or more hydroxyl and/or carboxylic acid groups, mass spectrometry data was acquired in negative ionisation mode.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 3b displays the UPLC-HRMS of ATPS, which is comparable to the HPLC and UPLC-HRMS with minor discrepancies potentially attributed to the HPLC utilising solely two wavelengths (285 nm, 210 nm) and variations in mobile phases and columns. Sanches et al (2022) conducted a comprehensive analysis of the chemical composition of three citrus peels, namely lime (Citrus × latifolia), lemon (Citrus limon), and rangpur lime (Citrus × limonia), and identified twenty-four polyphenols and five limonoids. As citrus peels are predominantly composed of polyphenols containing one or more hydroxyl and/or carboxylic acid groups, mass spectrometry data was acquired Ó 2023 Institute of Food Science and Technology.…”

Section: Identification Of Compounds Of Bioactive Constituents Escapingmentioning

confidence: 99%

See 1 more Smart Citation

Ethanol/salt aqueous two‐phase systems (ATPS) for enrichment and identification of bioactive constituents escaping during pectin extraction

Ruan

Jiang

Jin-song

et al. 2023

Int J of Food Sci Tech

View full text Add to dashboard Cite

SummaryThe acid heating extraction process is commonly employed to extract pectin from citrus peels, which results in the leaching of bioactive constituents into solvents. However, the discharge of these solvents represents a significant loss of valuable resources. To address this issue, this study aims to identify the composition and concentration of the bioactive constituents that escape into the solvents and propose an extraction process based on an ethanol‐salt aqueous two‐phase system (ATPS). The well‐designed ATPS enables the enrichment and recovery of the bioactive constituents, and the findings demonstrate that the ethanol/sodium citrate‐based ATPS can recover up to 80.61% of limonoids, 84.11% of flavonoids, and 88.09% of polyphenols that escape at an enrichment efficiency of 50.00%. Moreover, HPLC‐DAD and UPLC‐HRMS analysis of the ATPS extracts revealed the presence of nine bioactive constituents, including flavonoids, phenolic acids, and limonoids. Remarkably, in vitro antioxidant capacity studies indicate that the ATPS extracts exhibit superior antioxidant activity compared to the vacuum‐concentrated solution. Thus, these results suggest that the application of ATPS extraction is a feasible and promising strategy for the recovery of bioactive constituents.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Identification Of Compounds Of Bioactive Constituents Escapingmentioning

confidence: 99%

Ethanol/salt aqueous two‐phase systems (ATPS) for enrichment and identification of bioactive constituents escaping during pectin extraction

Ruan

Jiang

Jin-song

et al. 2023

Int J of Food Sci Tech

View full text Add to dashboard Cite

show abstract

“…Among the bioactive compounds of citrus fruit by‐products, essential oils and phenolic extracts have more popularity in either research or application 10 . A study reported that citrus peels of key lime, orange, sweet orange, tangerine, and tangerine ponkan species contained more than 24 phenolic compounds and at least 5 compounds of the limonoids group 11 . For volatile compounds in essential oils extracted from citrus peels, it has been reported that limonene, terpinene, and myrcene were the major compounds in peels of sweet orange, bitter orange, and mandarins 9 .…”

Section: Introductionmentioning

confidence: 99%

“…10 A study reported that citrus peels of key lime, orange, sweet orange, tangerine, and tangerine ponkan species contained more than 24 phenolic compounds and at least 5 compounds of the limonoids group. 11 For volatile compounds in essential oils extracted from citrus peels, it has been reported that limonene, terpinene, and myrcene were the major compounds in peels of sweet orange, bitter orange, and mandarins. 9 Whereas terpinene-4-ol and citronellal exhibited the highest concentration in key lime peel which was up to 34.58 μg g −1 .…”

Section: Introductionmentioning

confidence: 99%

Valorization of kaffir lime peel through extraction of essential oil and process optimization for phenolic compounds

Houng

Lay

2023

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUNDKaffir lime peel is a food waste which contains good aromatic essential oil and phenolic compounds. Extraction of essential oil from kaffir lime peel is conducted to obtain the extract for using as biological ingredients in various products. After essential oil extraction, kaffir lime peel residue has been remained, and it can be considered as another by‐product. Therefore, it is interesting to evaluate the contents of phenolic compounds in the kaffir lime peel residue to understand the possibility of valorization of kaffir lime peel wastes at a practical level. In this study, extraction of essential oil from fresh kaffir lime peel was conducted by hydrodistillation, and phenolic compounds were extracted from the kaffir lime peel residue by process optimization of an ethanolic extraction method.RESULTSThrough hydrodistillation and gas chromatography–mass spectrometry analysis, essential oil of kaffir lime peel contained terpinene‐4‐ol (19%), β‐pinene (15.8%) and d‐limonene (15.6%) as the major volatile compounds. In extraction of phenolic compounds from kaffir lime peel residue, all investigated parameters had significant linear effects on the yield of phenolic compounds, but no interaction effects among the extraction parameters were observed. Optimal conditions for the extraction were 120 min, 60 °C and 1:20 g mL−1, resulting in phenolic compounds of 8.6 mg GAE g−1 sample. The phenolic compound yield extracted from fresh kaffir lime peel at the optimal conditions was close to that extracted from the kaffir lime peel residue.CONCLUSIONSThrough hydrodistillation, only essential oil was extracted from kaffir lime peel, while phenolic compounds are still remained in the solid residue of the kaffir lime peel at high concentration as the fresh sample. Results of this study are useful for maximizing extraction yield of phenolic compounds from kaffir lime peel. The results of this study also revealed the possibility of maximizing the utilization of kaffir lime peel wastes at the practical stage through extraction of both essential oils and phenolic compounds. © 2023 Society of Chemical Industry (SCI).

show abstract

“…Due to their charac-teristics, fused-core particles generate a lower pressure, allowing smaller particles to improve the chromatographic resolution and speed up the analysis. Methods employing columns packed with fused-core particles can usually reduce analysis time by 3-4 times compared to conventional HPLC methods [27][28][29][30]. This particle technology addresses the slow mass transfer of solute molecules inside conventional particles by providing a small diffusion path into and out of the stationary phase, reducing the time molecules spend inside the particles.…”

Section: Introductionmentioning

confidence: 99%

Ultra‐high‐performance liquid chromatography using a fused‐core particle column for fast analysis of propolis phenolic compounds

Contieri

Mesquita

Sanches

et al. 2022

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Propolis is a bee product with a complex chemical composition formed by several species from different geographical origins. The complex propolis composition requires an accurate and reproducible characterization of samples to standardize the quality of the material sold to consumers. This work developed an ultra-highperformance liquid chromatography with a photodiode array detector method to analyze propolis phenolic compounds based on the two key propolis biomarkers, Artepillin C and p-Coumaric acid. This choice was made due to the complexity of the sample with the presence of several compounds. The optimized method was hyphenated with mass spectrometry detection allowing the detection of 23 different compounds. A step-by-step strategy was used to optimize temperature, flow rate, mobile phase composition, and re-equilibration time. Reverse-phase separation was achieved with a C18 fused-core column packed with the commercially available smallest particles (1.3 nm). Using a fused-core column with ultra-high-performance liquid chromatography allows highly efficient, sensitive, accurate, and reproducible determination of compounds extracted from propolis with an outstanding sample throughput and resolution. Optimized conditions permitted the separation of the compounds in 5.50 min with a total analysis time (sample-to-sample) of 6.50 min.

show abstract

Comprehensive analysis of phenolics compounds in citrus fruits peels by UPLC-PDA and UPLC-Q/TOF MS using a fused-core column

Cited by 30 publications

References 40 publications

Ethanol/salt aqueous two‐phase systems (ATPS) for enrichment and identification of bioactive constituents escaping during pectin extraction

Ethanol/salt aqueous two‐phase systems (ATPS) for enrichment and identification of bioactive constituents escaping during pectin extraction

Valorization of kaffir lime peel through extraction of essential oil and process optimization for phenolic compounds

Ultra‐high‐performance liquid chromatography using a fused‐core particle column for fast analysis of propolis phenolic compounds

Contact Info

Product

Resources

About