Comprehensive Analysis of Mouse Hippocampal Lysine Acetylome Mediated by Sea Cucumber Peptides Preventing Memory Impairment

Abstract

Memory impairment is becoming a potential health issue with the delicacy of diet and social stress. Sea cucumber peptides (SCP) prevent memory impairment, as previously reported. In this study, further research was performed using hippocampal lysine-acetylome to explore molecular regulation mechanisms. C57BL/6 mice were treated with scopolamine via intraperitoneal injection to simulate memory impairment. To determine the influence of SCP on the total acetylated-protein level of the hippocampus, acetylated-proteomics was performed. SCP increased the acetylation level of histone (H3 and H4). Meanwhile, for non-histones, the differentially acetylated proteins were involved in multiple memory-related pathways, as shown by KEGG enrichment analysis. Additionally, long-term potentiation was confirmed by western blotting. Finally, a combined analysis of proteome and lysine acetylome revealed that SCP contributed to synaptic vesicle cycle regulation and dopamine metabolism. Consequently, our findings revealed that SCP was potentially neuroprotective by regulating post-transcriptional hippocampal protein acetylation.

Gaussian Accelerated Molecular Dynamics Simulations Investigation on the Mechanism of Angiotensin-Converting Enzyme (ACE) C-Domain Inhibition by Dipeptides

“…As a result, foodderived ACE-inhibitory peptides have attracted increasing attention due to the fact of their milder side effects during treatment. Currently, ACE-inhibitory peptides can be obtained from dairy products [11,12], eggs [13], seafood [14][15][16], and plants [17][18][19]. Moreover, a number of ACE-inhibiting peptides derived from natural food materials [20,21] have been successfully developed as effective alternatives to synthetic drugs for the prevention of hypertension in a safer manner.…”

Sea Cucumber-Derived Peptide Attenuates Scopolamine-Induced Cognitive Impairment by Preventing Hippocampal Cholinergic Dysfunction and Neuronal Cell Death

“…These results are consistent with those of previous studies on sea cucumber hydrolysate. 27,37 A previous study conducted by Lee et al 38 suggested that obvious cognitive dysfunction was induced by scopolamine in mice. Wu et al 20 also revealed that the QMDDQ peptide derived from shrimp significantly decreased the escape latency of mice induced by scopolamine in the MWM test.…”

Novel peptides from sea cucumber intestinal hydrolysates promote longitudinal bone growth in adolescent mice through accelerating cell cycle progress by regulating glutamine metabolism

“…41 Lu et al found that the peptide from sea cucumber prevented memory impairment by regulating post-transcriptional hippocampal protein acetylation. 42 Likewise, another anti-cancer peptide isolated from soybean exerted bioactivities by inhibiting histone acetylation. 43 For growth plate chondrocytes, Stegen et al showed that glutamine promoted growth plate chondrocyte proliferation during endochondral ossification through acetylation modification of the histone 3K9.…”