Comprehensive analysis of miRNA and protein profiles within exosomes derived from canine lymphoid tumour cell lines

Abstract

Exosomes are small extracellular vesicles released from almost all cell types, which play roles in cell-cell communication. Recent studies have suggested that microenvironmental crosstalk mediated by exosomes is an important factor in the escape of tumour cells from the anti-tumour immune system in human haematopoietic malignancies. Here, we conducted comprehensive analysis of the miRNA and protein profiles within the exosomes released from four canine lymphoid tumour cell lines as a model of human lymphoid tumours. The results showed that the major miRNAs and proteins extracted from the exosomes were similar among the four cell lines. However, the miRNA profiles differed among the exosomes of each cell line, which corresponded to the expression patterns of the parent cells. In the comparison of the amounts of miRNAs and proteins among the cell lines, those of three miRNAs (miR-151, miR-8908a-3p, and miR-486) and CD82 protein differed between exosomes derived from vincristine-sensitive and resistant cell lines. Further investigations are needed to elucidate the biological functions of the exosomal contents in the microenvironmental crosstalk of lymphoid tumours.

CD5L as an Extracellular Vesicle-Derived Biomarker for Liquid Biopsy of Lung Cancer

“…Recent studies have revealed that the protein levels in EVs do not always reflect their levels in parent cells. For example, CD82 is not expressed in lymphoid tumor cells but is overexpressed in the EVs derived from these very same lymphoid cells [ 32 ]. Upon comparing the proteome profiles of EVs from three types of pancreatic cancer cells, it was found that pancreatic ductal adenocarcinoma (PDAC)-cell-derived EVs share less than 50% of their proteins with the parent cells [ 33 ].…”

Epigenetic Mechanisms in Canine Cancer

“…A recent study observed that canine mammary epithelial cancer cells shed exosomes that contained differentially expressed miRNAs in comparison with normal cells (139). In a study performed in canine lymphoma, three miRNAs (miR-151, miR-8908a-3p, and miR-486) derived from exosomes demonstrated to be differently expressed between vincristinesensitive and resistant lymphoma cell lines supporting a role of these miRNAs in the resistance of this cancer (140).…”

Exosomes in Age-Related Cognitive Decline: Mechanistic Insights and Improving Outcomes

“…Indeed, recent reports indicate neuronal exosomes can be isolated from saliva, while urinary exosomes can classify dementia patients from healthy controls ( Rani et al, 2019 ; Sun et al, 2019 ). Additionally, the diversity of exosomal content presents investigators with the unique opportunity to improve diagnostic capacity; compared to approaches which assess a single molecular target (e.g., proteins), exosomes can enable researchers to leverage information simultaneously from a variety of molecular targets (e.g., proteins, lipids, RNA, DNA) ( Thakur et al, 2014 ; Dinkins et al, 2017 ; Asada et al, 2019 ; Teruel-Montoya et al, 2019 ). However, comparisons across studies should be restricted to samples of the same biological fluid, given that preparations from different biological fluids incur differing degrees of contamination during preparation ( Grigor’eva et al, 2017 ).…”