Comprehensive analysis of lncRNA-mediated ceRNA networkfor hepatocellular carcinoma

Abstract

BackgroundHepatocellular carcinoma (HCC) is a high-burden cancer. The molecular mechanism of HCC has not been fully elucidated. Notably, current research has revealed a significant function for long non-coding RNAs (lncRNAs) in the prognosis of patients with HCC. Here, this study aims to construct a regulated lncRNA-mediated ceRNA network and find biological targets for the treatment of HCC.MethodsBased on the RNA expression patterns from the TCGA, we did an analysis to determine which genes were expressed differently between liver tumor tissues and noncancerous tissues. Then, using bioinformatic tools, we built a lncRNA-miRNA-mRNA ceRNA network and used GO and KEGG functional analyses on the DEmRNAs connected to ceRNA networks. The main lncRNAs in the subnetwork were chosen, and we next looked at the relationships between these lncRNAs and the clinical characteristics of patients with HCC. The prognosis-related genes and immune cells were identified using Kaplan-Meier and Cox proportional hazard analyses, and CIBERSORT was utilized to separate the 22 immune cell types. CCK8 assay was performed to measure cell viability in HCC cells after lncRNA HOTTIP modulation.ResultsDifferentially expressed mRNA and lncRNAs in HCC and paracancerous tissues were identified. There are 245 lncRNAs, 126 miRNAs, and 1980 mRNAs that are expressed differently in liver tumour tissues than in noncancerous cells. Function analysis showed that mRNAs in ceRNA network were significantly enriched in G1/S transition of mototiv cell cycle, positive regulation of cell cycle process, hepatocellular carcinoma, and cancer related pathways. CD8 T cells and T follicular helper cells had a favourable link with a 0.65 correlation coefficient. Additionally, there was a strong correlation between Eosinophils, activated NK cells, and B memory cells. Strikingly, depletion of lncRNA HOTTIP inhibited viability of HCC cells. In addition, miR-205 upregulation suppressed viability of HCC cells, while miR-205 downregulation repressed viability of HCC cells. Notably, miR-205 depletion rescued HOTTIP depletion-mediated suppression of cell viability in HCC.ConclusionA ceRNA network was created by examining the lncRNA, miRNA, and mRNA expression profiles of liver tumours from the TCGA database. LncRNA HOTTIP promoted cell viability via inhibition of miR-205 in HCC cells.

Long noncoding RNA THOR, a novel target biomolecule, is involved in the progression of colorectal cancer

“…About 98% of RNA in the human genome is non-coding RNA, which was previously considered as the "noise" of gene transcription [10,11]. Long noncoding RNAs (lncRNAs) are noncoding RNAs with a length of more than 200 bp and are widely found in the nucleus and cytoplasm [12]. In recent years, lncRNAs have been found to participate in various biological processes such as cell cycle, cell growth, cell differentiation and apoptosis through transcriptional regulation, translation regulation, RNA editing, chromosome remodeling, microRNA sponging and other activities; they also play a role in the regulation of many pathophysiological processes, such as cancer and metabolic diseases, as molecular signals, scaffolds, enhancer or inhibitors [13][14][15].…”

The role of pyroptosis-related lncRNA risk signature in ovarian cancer prognosis and immune system

“…Numerous studies have demonstrated that long non-coding RNA (lncRNA) plays a major role in regulatory functions in carcinogenesis [ 26 – 29 ]. LncRNAs are the RNAs that have length longer than 200 nt, and previously was believed to be transcriptional noise [ 30 ]. For example, GAS5 inhibits ovarian cancer development by inducing inflammation response [ 31 , 32 ].…”