Comprehensive analysis of human IgG Fc N-glycopeptides and construction of a screening model for colorectal cancer

Zou, Yang; Hu, Jianxing; Jie, Jianzheng; Lai, Junyong; Li, Mingna; Liu, Zhenming; Zou, Xiajuan

doi:10.1016/j.jprot.2019.103616

Cited by 12 publications

(11 citation statements)

References 57 publications

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“…Moreover, cancer-derived Ig can be detected by the RP215 antibody in the serum of cancer patients and shows considerable value in monitoring cancer progression ( 95 ). Recently, serum IgG glycosylation has been identified as having great significance in differential diagnosis, disease progression monitoring, and therapeutic efficacy prediction in cancer patients ( 96 – 100 ). Similarly, autoantibodies have also been considered next generation biomarkers of cancers ( 101 – 104 ).…”

Section: Clinical Significance Of Cancer-derived Ig In Cancermentioning

confidence: 99%

Immunoglobulin Expression in Cancer Cells and Its Critical Roles in Tumorigenesis

et al. 2021

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Traditionally, immunoglobulin (Ig) was believed to be produced by only B-lineage cells. However, increasing evidence has revealed a high level of Ig expression in cancer cells, and this Ig is named cancer-derived Ig. Further studies have shown that cancer-derived Ig shares identical basic structures with B cell-derived Ig but exhibits several distinct characteristics, including restricted variable region sequences and aberrant glycosylation. In contrast to B cell-derived Ig, which functions as an antibody in the humoral immune response, cancer-derived Ig exerts profound protumorigenic effects via multiple mechanisms, including promoting the malignant behaviors of cancer cells, mediating tumor immune escape, inducing inflammation, and activating the aggregation of platelets. Importantly, cancer-derived Ig shows promising potential for application as a diagnostic and therapeutic target in cancer patients. In this review, we summarize progress in the research area of cancer-derived Ig and discuss the perspectives of applying this novel target for the management of cancer patients.

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Section: Clinical Significance Of Cancer-derived Ig In Cancermentioning

confidence: 99%

Immunoglobulin Expression in Cancer Cells and Its Critical Roles in Tumorigenesis

et al. 2021

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“…Detection of IgG Fc N -glycopeptides by nanomaterial enrichment with subsequent MALDI-TOF mass spectrometry (MS) analysis of plasma samples from 46 CRC patients and 67 healthy individuals was evaluated in the form of an ROC curve. Machine learning algorithm using analysis of 11 N -glycopeptides was able to distinguish CRC patients from healthy controls with the average AUC value of the ROC of 0.893 ( 57 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray

et al. 2021

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Colorectal cancer (CRC) is one of the most common types of cancer among men and women worldwide. Efforts are currently underway to find novel and more cancer-specific biomarkers that could be detected in a non-invasive way. The analysis of aberrant glycosylation of serum glycoproteins is a way to discover novel diagnostic and prognostic CRC biomarkers. The present study investigated a whole-serum glycome with a panel of 16 different lectins in search for age-independent and CRC-specific glycomarkers using receiver operating characteristic (ROC) curve analyses and glycan heat matrices. Glycosylation changes present in the whole serum were identified, which could lead to the discovery of novel biomarkers for CRC diagnostics. In particular, the change in the bisecting glycans (recognized by Phaseolus vulgaris erythroagglutinin) had the highest discrimination potential for CRC diagnostics in combination with human L selectin providing area under the ROC curve (AUC) of 0.989 (95% CI 0.950–1.000), specificity of 1.000, sensitivity of 0.900, and accuracy of 0.960. We also implemented novel tools for identification of lectins with strong discrimination power.

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“…Many proteins undergo co-or post-translational modifications, including phosphorylation, acetylation, and glycosylation to fulfill their functions (Walsh and Jefferis, 2006;Carubbi et al, 2019). It is estimated that glycosylation modifications are associated with approximately 50% of human proteins (Clerc et al, 2016;Oliveira-Ferrer et al, 2017) and 30% of approved biopharmaceutical proteins (Zou et al, 2020), which are critical for important biological processes in living systems, such as cell's adhesion, recognition, targeting, and differentiation (Varki, 2017;Bhat et al, 2019). Despite the importance of glycosylations, rigorous evaluation of the relationship between the precise structure and biological function of glycoproteins is complicated by the structural heterogeneity of the oligosaccharides in biological organisms, and the difficulty to obtain sufficient amounts of structure-defined glycoproteins with single glycoform from natural sources (Park et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides

Gao

Sun

et al. 2020

Front. Chem.

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Chemical synthesis is an attractive approach allows for the assembly of homogeneous complex N-linked glycopeptides and glycoproteins, but the limited coupling efficiency between glycans and peptides hampered the synthesis and research in the related field. Herein we developed an alternative glycosylation to construct N-linked glycopeptide via efficient selenoester-assisted aminolysis, which employs the peptidyl ω-asparagine selenoester and unprotected glycosylamine to perform rapid amide-bond ligation. This glycosylation strategy is highly compatible with the free carboxylic acids and hydroxyl groups of peptides and carbohydrates, and readily available for the assembly of structure-defined homogeneous N-linked glycopeptides, such as segments derived from glycoprotein EPO and IL-5.

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Comprehensive analysis of human IgG Fc N-glycopeptides and construction of a screening model for colorectal cancer

Cited by 12 publications

References 57 publications

Immunoglobulin Expression in Cancer Cells and Its Critical Roles in Tumorigenesis

Immunoglobulin Expression in Cancer Cells and Its Critical Roles in Tumorigenesis

Identification of Whole-Serum Glycobiomarkers for Colorectal Carcinoma Using Reverse-Phase Lectin Microarray

Peptidyl ω-Asp Selenoesters Enable Efficient Synthesis of N-Linked Glycopeptides

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