Comprehensive analysis of gene expression patterns of hedgehog-related genes

Hao, Limin; Johnsen, Robert; Lauter, Gilbert; Baillie, David L.; Bürglin, Thomas R.

doi:10.1186/1471-2164-7-280

Cited by 82 publications

(80 citation statements)

References 38 publications

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“…Consistent with a role in epidermal activities, the seam cells express 52 collagen genes, several other molting genes, and eight hedgehog-like genes that are known to function in this tissue (Hao et al 2006) (Table S3). In contrast with the hypodermis, the seam cells express a much smaller pool of 105 unique genes (Figure 2B).…”

Section: Resultsmentioning

confidence: 83%

Alternative Polyadenylation Directs Tissue-Specific miRNA Targeting in Caenorhabditis elegans Somatic Tissues

et al. 2017

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Section: Resultsmentioning

confidence: 83%

Alternative Polyadenylation Directs Tissue-Specific miRNA Targeting in Caenorhabditis elegans Somatic Tissues

et al. 2017

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“…To validate this result we examined expression of two other seam cell markers, grd-10p::gfp (Hao et al 2006) and egl-18p::mCherry (Liu et al 2009), in pax-3(RNAi) animals. grd-10 encodes a hedgehog-like protein that is expressed in seam cells in all developmental stages (Aspöck et al 1999).…”

Section: Resultsmentioning

confidence: 99%

The Paired-box protein PAX-3 regulates the choice between lateral and ventral epidermal cell fates in C. elegans

Thompson

Joshi

Dymond

et al. 2016

Developmental Biology

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The development of the single cell layer skin or hypodermis of Caenorhabditis elegans is an excellent model for understanding cell fate specification and differentiation. Early in C. elegans embryogenesis, six rows of hypodermal cells adopt dorsal, lateral or ventral fates that go on to display distinct behaviors during larval life. Several transcription factors are known that function in specifying these major hypodermal cell fates, but our knowledge of the specification of these cell types is sparse, particularly in the case of the ventral hypodermal cells, which become Vulval Precursor Cells and form the vulval opening in response to extracellular signals. Previously, the gene pvl-4 was identified in a screen for mutants with defects in vulval development. We found by whole genome sequencing that pvl-4 is the Paired-box gene pax-3, which encodes the sole PAX-3 transcription factor homolog in C. elegans. pax-3 mutants show embryonic and larval lethality, and body morphology abnormalities indicative of hypodermal cell defects. We report that pax-3 is expressed in ventral P cells and their descendants during embryogenesis and early larval stages, and that in pax-3 reduction-of-function animals the ventral P cells undergo a cell fate transformation and express several markers of the lateral seam cell fate. Furthermore, forced expression of pax-3 in the lateral hypodermal cells causes them to lose expression of seam cell markers. We propose that pax-3 functions in the ventral hypodermal cells to prevent these cells from adopting the lateral seam cell fate. pax-3 represents the first gene required for specification solely of the ventral hypodermal fate in C. elegans providing insights into cell type diversification.

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“…GPN-1 is required to promote some cell migrations, but animals lacking functional GPN-1 have a wild-type body length, indicating that GPN-1 does not normally play a role in this BMP-mediated phenotype (Gumienny et al, 2007; Hudson et al, 2006). However, gpn-1 is expressed in different tissues and at different times than lon-2 (Hao et al, 2006; Hudson et al, 2006; Hunt-Newbury et al, 2007; McKay et al, 2003). We asked if GPN-1 could functionally replace LON-2 by driving expression of gpn-1 cDNA from the lon-2 promoter sequence.…”

Section: Resultsmentioning

confidence: 99%

Two functional domains in C. elegans glypican LON-2 can independently inhibit BMP-like signaling

Taneja-Bageshwar¹,

Gumienny²

2012

Developmental Biology

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Glypicans are multifunctional proteoglycans with regulatory roles in several intercellular signaling pathways. Here, we examine the functional requirements for glypican regulation of bone morphogenetic protein (BMP)-mediated body length in C. elegans. We provide evidence that two parts of C. elegans glypican LON-2 can independently inhibit BMP signaling in vivo: the N-terminal furin protease product and the C-terminal region containing heparan sulfate attachment sequences. While the C-terminal protease product is dispensable for LON-2 minimal core protein activity, it does affect the localization of LON-2. Cleavage of LON-2 into two parts at the conserved furin protease site is not required for LON-2 to inhibit BMP-like signaling. The glycosyl-phosphatidylinositol (GPI) membrane anchor is also not absolutely required for LON-2 activity. Finally, we show that an RGD protein–protein interaction motif in the LON-2 N-terminal domain is necessary for LON-2 core protein activity, suggesting that LON-2 inhibits BMP signaling by acting as a scaffold for BMP and an RGD-binding protein.

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Comprehensive analysis of gene expression patterns of hedgehog-related genes

Cited by 82 publications

References 38 publications

Alternative Polyadenylation Directs Tissue-Specific miRNA Targeting in Caenorhabditis elegans Somatic Tissues

Alternative Polyadenylation Directs Tissue-Specific miRNA Targeting in Caenorhabditis elegans Somatic Tissues

The Paired-box protein PAX-3 regulates the choice between lateral and ventral epidermal cell fates in C. elegans

Two functional domains in C. elegans glypican LON-2 can independently inhibit BMP-like signaling

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