Comprehensive Analysis of Blood Coagulation Pathways in Teleostei: Evolution of Coagulation Factor Genes and Identification of Zebrafish Factor VIIi

Hanumanthaiah, Ravikumar; Day, Kenneth; Jagadeeswaran, Pudur

doi:10.1006/bcmd.2002.0534

Cited by 70 publications

(68 citation statements)

References 25 publications

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“…78 Zebrafish possess all the major proteins of the coagulation system, including both vitamin K-dependent procoagulant factors, anticoagulant factors, and the fibrinolytic pathway. 81 The function of the system has been measured with assays equivalent to the activated partial thromboplastin time, prothrombin time, and dilute Russell Viper Venom Time (DRVVT). 81,82 Vitamin K carboxylase is present and warfarin prolongs the prothrombin time in a dose-dependent fashion when administered via the tank water.…”

Section: Morphology and Physiology Of Zebrafish Blood Cellsmentioning

confidence: 99%

“…81 The function of the system has been measured with assays equivalent to the activated partial thromboplastin time, prothrombin time, and dilute Russell Viper Venom Time (DRVVT). 81,82 Vitamin K carboxylase is present and warfarin prolongs the prothrombin time in a dose-dependent fashion when administered via the tank water. 83 …”

Section: Morphology and Physiology Of Zebrafish Blood Cellsmentioning

confidence: 99%

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Zebrafish in hematology: sushi or science?

Carradice

Lieschke

2008

Blood

155

134

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IntroductionZebrafish (Danio rerio) have contributed to hematologic research for more than 50 years. Interest in zebrafish embryology dates to the 1930s, 1 and they have long been used for zoology and toxicology research. 2 Their developmental hematology entered the literature in 1963 with the second appearance of zebrafish in the journal Nature, as a representative teleost fish with an intraembryonic origin for blood. 3 The first descriptions of zebrafish blood cell morphology appeared in the 1970s. [4][5][6] The "modern" phase of zebrafish hematology research, driven by genetic experimental approaches, started just over 10 years ago with the collection of zebrafish mutants with hematopoietic defects, mostly recognized for their anemia. [7][8][9] Genetic approaches to studying hematopoiesis were pioneered in the mouse. Originally, spontaneous mutants provided the majority of models, 10 but functional studies of specific genes, using transgenic and gene targeting methods in vivo, have dominated murine research for the last 25 years. However, the genes selected for such reverse genetic functional analyses are by definition biased by prior knowledge, and a reverse genetic gene-by-gene approach is unlikely to reveal the full set of genes contributing to hematopoiesis. Indeed, the genetic basis of many congenital human blood diseases remains unknown.Unbiased forward genetic approaches, particularly saturation mutagenesis and phenotype-based screening for mutants, sample the genome for all genetically interruptible, functionally mandatory steps in complex biologic pathways and have been of proven value in nonmammalian models (eg, Caenorhabditis elegans, Drosophila). 11 Unfortunately, the hematologic system of Drosophila is different from that of vertebrates such as mammals. 12 At a genetic level, hematologically important transcription factor families and signaling pathways are represented, 12 but at a cellular level, Drosophila hematology is primitive compared with mammals and adaptive immunity is entirely lacking.Outcomes from forward genetic screens in mice looking for hematopoietic mutants are now being reported, with mutants in genes both expected (Ikaros,14 Gata-1 15 ) and unexpected (C1galt1, 16 Bcl-x(L) 17 ). Although these examples show that murine genetic screens are feasible, they are logistically difficult because of their high cost and resource requirements and hence are typically scaled well below saturation screening and are currently limited to a few large projects.Zebrafish combine an affordable, genetically tractable vertebrate model with biology particularly suited to studying early development (eg, rapid ex vivo development, high fecundity, and optical transparency). Key reverse genetic techniques elsewhere reviewed 18,19 for functionally studying genes of interest in zebrafish include transient gene overexpression and knockdown, stable transgenesis, 20 and recovering stable mutated alleles by TILLING (Targeting Induced Local Lesions In Genomes), 21 or from catalogued libraries of insertional muta...

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Section: Morphology and Physiology Of Zebrafish Blood Cellsmentioning

confidence: 99%

Section: Morphology and Physiology Of Zebrafish Blood Cellsmentioning

confidence: 99%

Zebrafish in hematology: sushi or science?

Carradice

Lieschke

2008

Blood

155

134

View full text Add to dashboard Cite

show abstract

“…[9][10][11][12] Zebrafish (Danio rerio) have been widely used to study hemostasis, demonstrating conservation and function of the structural components of coagulation, including fibrinogen 13,14 and von Willebrand factor, 15,16 and the ability to develop thrombosis in response to a laser-induced injury. 14,17 Embryonic development is external, rapid, and transparent, allowing unfettered access to studies of the circulatory system.…”

Section: Introductionmentioning

confidence: 99%

Targeted mutagenesis of zebrafish antithrombin III triggers disseminated intravascular coagulation and thrombosis, revealing insight into function

Liu¹,

Kretz

Maeder

et al. 2014

Blood

108

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“…It was already known that genes for these two proteins are present in jawed fishes like the pufferfish (Davidson et al 2003a;Jiang and Doolittle 2003) and zebrafish (Hanumanthaiah et al 2002), and the proposal was that in the interval between the divergence of the jawless and jawed fish more or less simultaneous duplications of genes for factors X and V gave rise to those for factors IX and VIII. Crucially, factors VIII and IX interact with each other in the same way that factors V and X do, the first-named of each pair (VIII and V) serving as a large molecular weight co-factor for the second-named vitamin K-dependent protease (factors IX and X).…”

Section: Introductionmentioning

confidence: 99%

Bioinformatic Characterization of Genes and Proteins Involved in Blood Clotting in Lampreys

Doolittle

2015

J Mol Evol

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Lampreys and hagfish are the earliest diverging of extant vertebrates and are obvious targets for investigating the origins of complex biochemical systems found in mammals. Currently, the simplest approach for such inquiries is to search for the presence of relevant genes in whole genome sequence (WGS) assemblies. Unhappily, in the past a high-quality complete genome sequence has not been available for either lampreys or hagfish, precluding the possibility of proving gene absence. Recently, improved but still incomplete genome assemblies for two species of lamprey have been posted, and, taken together with an extensive collection of short sequences in the NCBI trace archive, they have made it possible to make reliable counts for specific gene families. Particularly, a multi-source tactic has been used to study the lamprey blood clotting system with regard to the presence and absence of genes known to occur in higher vertebrates. As was suggested in earlier studies, lampreys lack genes for coagulation factors VIII and IX, both of which are critical for the ''intrinsic'' clotting system and responsible for hemophilia in humans. On the other hand, they have three each of genes for factors VII and X, participants in the ''extrinsic'' clotting system. The strategy of using raw trace sequence ''reads'' together with partial WGS assemblies for lampreys can be used in studies on the early evolution of other biochemical systems in vertebrates.

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Comprehensive Analysis of Blood Coagulation Pathways in Teleostei: Evolution of Coagulation Factor Genes and Identification of Zebrafish Factor VIIi

Cited by 70 publications

References 25 publications

Zebrafish in hematology: sushi or science?

Zebrafish in hematology: sushi or science?

Targeted mutagenesis of zebrafish antithrombin III triggers disseminated intravascular coagulation and thrombosis, revealing insight into function

Bioinformatic Characterization of Genes and Proteins Involved in Blood Clotting in Lampreys

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