Comprehensive Analysis of a Vibrio parahaemolyticus Strain Extracellular Serine Protease VpSP37

Abstract

Proteases play an important role in the field of tissue dissociation combined with regenerative medicine. During the years new sources of proteolytic enzymes have been studied including proteases from different marine organisms both eukaryotic and prokaryotic. Herein we have purified a secreted component of an isolate of Vibrio parahaemolyticus, with electrophoretic mobilities corresponding to 36 kDa, belonging to the serine proteases family. Sequencing of the N-terminus enabled the in silico identification of the whole primary structure consisting of 345 amino acid residues with a calculated molecular mass of 37.4 KDa. The purified enzyme, named VpSP37, contains a Serine protease domain between residues 35 and 276 and a canonical Trypsin/Chimotrypsin 3D structure. Functional assays were performed to evaluate protease activity of purified enzyme. Additionally the performance of VpSP37 was evaluated in tissue dissociations experiments and the use of such enzyme as a component of enzyme blend for tissue dissociation procedures is strongly recommended.

A GntR Family Transcription Factor (VPA1701) for Swarming Motility and Colonization of Vibrio parahaemolyticus

“…parahaemolyticus is a foodborne pathogen sometimes found in contaminated seafood, which can cause acute-gastroenteritis and hemorrhagic sepsis in humans [1,2]. The major virulence factors of V. parahaemolyticus are hemolysin (thermostable direct hemolysin [TDH], TDH-related hemolysin [TRH]), type III secretion systems (T3SS1 and T3SS2), type VI secretion systems (T6SS1 and T6SS2), biofilm formation, motility, protease production and iron uptake system [3][4][5][6][7][8]. The pathogenesis processes of V. parahaemolyticus include motility, adhesion, invasion, proliferation, production of toxins in vivo, and the damage to cells and tissues [9,10].…”

Settlement performance of the Mediterranean reef-builders Dendropoma cristatum (Biondi 1859) in response to natural bacterial films

“…This study shows the presence in the two artificially cultured biofilms of two Vibrio isolates, that may have inhibited the attachment of Dendropoma juveniles by proteases, largely produced by several Vibrio spp. (Salamone et al, 2015).…”

Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine

“…This study assesses the relative contribution of each collagenase in cartilage digestion, and seeks to establish an optimized protocol for NC isolation from cartilage using recombinant collagenases, namely ColG and ColH [18][19][20][21][22][23], in order to obtain highly viable cells which will be useful in producing large amounts of ECM component.…”