Compounds without borders: a novel paradigm for quantifying complex odors and responses to scent-pollution in bumblebees

Abstract

Bumblebees are critical pollinators whose populations have been experiencing troubling declines over the past several decades. Successful foraging improves colony fitness, thus understanding how anthropogenic influences modulate foraging behavior may aid conservation efforts. Odor pollution can have negative impacts on bumble-and honey-bees foraging behavior. However, given the vast array of potential scent contaminants, individually testing pollutants is an ineffective approach. The ability to quantitatively measure how much scent-pollution of a floralodor bumblebees can tolerate would represent a paradigm shift in odor-pollution studies. Current statistical methods for analyzing complex odors have poor predictive power because statisticallyderived odor-spaces are rewritten when new odors are added. This study presents an alternative method of analyzing complex odor blends based on the encoding properties of insect olfactory systems. This "Compounds Without Borders" (CWB) method vectorizes odors in a multidimensional space representing relevant functional group and carbon characteristics of their component odorants. A single vector can be built for any scent, which allows the angular distance between any two odors to be calculated -including a learned odor and its polluted counterpart. Data presented here indicate that CWB-angles are capable of both describing and predicting bumblebee odor-discrimination behavior: odor pairs with angular distances in the 20-29° range are generalized, while odor pairs over 30 degrees are differentiated. The neurophysiological properties underlying CWB-vectorization of odors are not unique to bumblebees; CWB-angle analysis of published data on a hawkmoth supports the idea that this method may have broader applications.