Compounds from the Roots of <i>Codonopsis pilosula</i> and Their SIRT1 Regulatory Activity

Zheng, Tao; Cheng, Likun; Yan, Yong‐Ming; Xu, Furong; Tang, Xiaolong; Li, Xiaozhen; Qin, Fu-Ying; Liu, Baohua; Cheng, Yong‐Xian

doi:10.1177/1934578x1801300112

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…The tubers of C. convolvulacea, as found in our results, were rich in amino acids and derivatives, free fatty acids, organic acids, phenolic acids, saccharides, alcohols, and vitamins. This is consistent with the earlier reports on C. lanceolata and C. peninsula ( Du et al, 2018 ; Zheng et al, 2018 ). The compositional analyses of the roots/tubers of these two species have also shown the higher accumulation (or the presence) of amino acids, vitamins, phenolic acids, saccharides, and alcohols.…”

Section: Discussionsupporting

confidence: 94%

Transcriptome and Metabolome Analyses of Codonopsis convolvulacea Kurz Tuber, Stem, and Leaf Reveal the Presence of Important Metabolites and Key Pathways Controlling Their Biosynthesis

Yuan

Zhao

et al. 2022

Front. Genet.

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Codonopsisconvolvulacea Kurz. var. vinciflora (Kom.) L.T. Shen is a member of Campanulaceae, which is used in traditional Chinese medicine. However, apart from a few Codonopsis species, no detailed knowledge is available on the metabolite composition and respective transcriptome signatures. We performed a combined transcriptome and metabolome analysis of the tuber, stem, and leaf of C. convolvulacea and found 1,144 metabolites and 231,840 unigenes in three experimental groups. The analysis revealed considerable variations in the three tissues. Tubers were rich in amino acids and derivatives, flavonoids, and organic acids, whereas the stems and leaves were rich in alkaloids and flavonoids, respectively. Transcriptome sequencing revealed candidate genes being involved in flavonoid, tryptophan, and alkaloid biosyntheses. In particular, we indicated that the variation in the isoflavone content is linked to the expressions of CHI, CYP73A, C3′H, F3H, CYP75B1, anthocyanidin synthase, and FLS. In a similar way, the levels of indole, L-tyrosine, and tryptamine were also consistent with the expressions of TDC/DDCs in the respective tissues. In addition, the expression levels of ASP5, ARO8, GOT, and AOC3 indicated that L-tryptophan is being converted to downstream metabolites. Overall, our datasets present a useful resource for future research on the uses of this medicinal plant and put forward many research questions.

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Section: Discussionsupporting

confidence: 94%

Transcriptome and Metabolome Analyses of Codonopsis convolvulacea Kurz Tuber, Stem, and Leaf Reveal the Presence of Important Metabolites and Key Pathways Controlling Their Biosynthesis

Yuan

Zhao

et al. 2022

Front. Genet.

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“…The effectiveness of the surtuin1 modulator could be demonstrated through sirtuin1 enzyme-stimulating activities. Literature suggested that a surtuin1 modulator could be a natural polyphenol extract from roots of codonopsis pilosula, kaempferia parviflora, drosophila melanogaster and fruits of lycium ruthenicun (Kayashima et al, 2017;Nakata et al, 2014;Qi et al, 2018;Zheng et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparison of pulse electric field, microwave and ultrasonic pretreatment prior to black rice extraction on antioxidant and sirtuin1 enzyme stimulating activities

et al. 2023

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The objective of this research was to investigate the effects of pulse electric field, microwave, and ultrasonic assisted water extractions on the bioactive compounds in black rice, and their antioxidant and sirtuin1 enzyme stimulating activities. The number of pulses were changed from 1,000 to 2,000 and 3,000 at a constant electric field intensity of 5 kV/cm. Microwave and ultrasonic conditions were followed by 800 W for 20 min and 60 °C for 20 min, respectively. The results found that using pulse electric field with 3,000 pulses and microwave achieved high concentrations of total phenolic compound and cyanidin-3-glucoside with non-significantly difference. Extracts from the pulse electric field with 3,000 pulses showed the significantly highest DPPH scavenging activity (30.83 ± 2.06%) and the sirtuin1 enzyme stimulating activity (27.260 ± 0.418 of fluorescence intensity) when compared with microwave and ultrasonic extractions. Scanning electron microscopy and fourier transform infrared spectroscopy results showed distinct differences between the effects of these methods on the outer surface. The overall results revealed that pulse electric field and microwave were similarly effective at increasing the content of bioactive compounds in extract and their antioxidant activities. However, the pulse electric field was considerably more effective for activating sirtuin1 enzyme activity.

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“…Moreover, the polyphenols have effect of anti-aging due to activation of via regulation of some signaling pathways, such as NF-κB 6 . Previously literatures represented that natural extract had able to activate sirtuin1 enzyme stimulation activities, such as polymethoxyflavonoids from Kaempferia parviflora 7 , drosophila melanogaster 8 , flavonoid glucoside from the fruits of lycium ruthenicun 9 , and roots of codonopsis pilosula 10 . Especially, anthocyanins consisted of cyanidin-3-glucoside (C3G) and peonidin-3-glucoside (P3G) in pigmented rice exhibited anti-inflammatory effects by inhibiting NF-κB and ERK/MAPK signaling pathway 11 .…”

Section: Introductionmentioning

confidence: 99%

Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities

Salee

Chaiyana

Yawootti

et al. 2022

Sci Rep

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Cyanidin-3-glucoside (C3G) and peonidin-3-glucoside (P3G) in black rice grain (BRG) demonstrate many beneficial health effects, including antioxidant and anti-aging properties. This research aimed to study on pulsed electric field assisted water extraction (PEF-AWE) on BRG using pre-treatment technique, which was determined for enhanced yields of C3G and P3G, antioxidant and sirtuin1 enzyme stimulation activities. The effects of operating parameters for PEF-AWE (intensity of electric field, X1: 3–5 kV/cm, number of pulse, X2: 1000–3000 pulse and BRG/water ratio, X3: 0.1–0.5 g/mL) were determined using regression analysis and optimized PEF-AWE condition using the response surface methodology. Regression models showed the intensity of electric field and BRG/water ratio were the strong influence parameters significantly on C3G (p < 0.01). The results highlighted optimized conditions of PEF-AWE followed by 5 kV/cm, 3000 pulse and 0.5 g/mL leading to achieve higher C3G (92.59 ± 4.79 mg/g) and P3G (4.59 ± 0.27 mg/g) than no pre-treatment by PEF process, approximately 60%. Additionally, PEF extracts of BRG can modulate the ability of surtuin1 enzyme to deacetylate substrate proteins (26.78 ± 0.50 FIR). Thus, PEF-AWE can be applied to produce BRG extract as natural antioxidant compound and functional ingredient.

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Compounds from the Roots of Codonopsis pilosula and Their SIRT1 Regulatory Activity

Cited by 4 publications

References 22 publications

Transcriptome and Metabolome Analyses of Codonopsis convolvulacea Kurz Tuber, Stem, and Leaf Reveal the Presence of Important Metabolites and Key Pathways Controlling Their Biosynthesis

Transcriptome and Metabolome Analyses of Codonopsis convolvulacea Kurz Tuber, Stem, and Leaf Reveal the Presence of Important Metabolites and Key Pathways Controlling Their Biosynthesis

Comparison of pulse electric field, microwave and ultrasonic pretreatment prior to black rice extraction on antioxidant and sirtuin1 enzyme stimulating activities

Optimization of the pulse electric field assisted extraction of black rice grain for antioxidant and sirtuin1 enzyme stimulation activities

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