Compounds fromSedum caeruleumwith antioxidant, anticholinesterase, and antibacterial activities

Abstract

Context: This is the first study on the phytochemistry, antioxidant, anticholinesterase, and antibacterial activities of Sedum caeruleum L. (Crassulaceae). Objective: The objective of this study is to isolate the secondary metabolites and determine the antioxidant, anticholinesterase, and antibacterial activities of S. caeruleum. Materials and methods: Six compounds (1-6) were isolated from the extracts of S. caeruleum and elucidated using UV, 1D-, 2D-NMR, and MS techniques. Antioxidant activity was investigated using DPPH , CUPRAC, and ferrous-ions chelating assays. Anticholinesterase activity was determined against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes using the Ellman method. Antibacterial activity was performed according to disc diffusion and minimum inhibitory concentration (MIC) methods. Results: Isolated compounds were elucidated as ursolic acid (1), daucosterol (2), b-sitosterol-3-O-b-D-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6). The butanol extract exhibited highest antioxidant activity in all tests (IC 50 value: 28.35 ± 1.22 mg/mL in DPPH assay, IC 50 value: 40.83 ± 2.24 mg/L in metal chelating activity, and IC 50 value: 23.52 ± 0.44 mg/L in CUPRAC), and the highest BChE inhibitory activity (IC 50 value: 36.89 ± 0.15 mg/L). Moreover, the chloroform extract mildly inhibited (MIC value: 80 mg/mL) the growth of all the tested bacterial strains. Discussion and conclusion: Ursolic acid (1), daucosterol (2), b-sitosterol-3-O-b-D-galactopyranoside (3), apigenin (4), apigetrin (5), and apiin (6) were isolated from Sedum caeruleum for the first time. In addition, a correlation was observed between antioxidant and anticholinesterase activities of bioactive ingredients of this plant.

Antimicrobial activity of flavonoids from Sedum aizoon L. against Aeromonas in culture medium and in frozen pork

“…(Crassulaceae) delayed the growth of bacteria. Similarly, Bensouici et al () found that the activities of Escherichia coli , Pseudomonas aeruginosa, and Staphylococcus aureus were obviously inhibited when treated with flavonoids from Sedum caeruleum L. (Crassulaceae). In our present work, the results of growth and time–kill curves showed that flavonoids from S. aizoon L. could prevent the logarithmic long‐term proliferation of Aeromonas .…”

Antioxidant and Anti-Inflammatory Flavonoids from the Flowers of Chuju, a Medical Cultivar of Chrysanthemum Morifolim Ramat

“…Thus, further purification was focused on the two flavonoids-enriched fractions (C and D). The isolated compounds ( Fig.1) included quercetin (1) [16], isorhamnetin 3-O-b-D-glucoside (2) [17], eriodictyol (3) [18], pyracanthoside (4) [19], apigenin (5) [20], apigetrin (6) [20], acacetin (7) [21], acacipetalin (8) [21], luteolin (9) [16], diosmetin (10) [22], spinacetin (11) [16], axillarin (12) [16], bonanzin (13) [23], cirsiliol (14) [24], chrysosplenol D (15) [25] and artemetin (16) [23], and were characterized by detailed spectroscopic analysis and comparing with the previous literature data. Among them, compounds 11-16 have never been reported from Chrsanthemum species before, and 14 compounds (1-3, 5-7, 9-16) were isolated from Chuju for the first time.…”

Flavonoids, cytotoxic, antioxidant and antibacterial activities of Evax pygmaea

“… 2004 ; Bensouici et al. 2016 ). One millilitres of copper (II) chloride solution (0.01 M prepared from CuCl 2 ·2H 2 O), 1 mL of ammonium acetate buffer at pH 7.0 and 1 mL of neocaproin solution (0.0075 M) were mixed to 0.5 mL of plant extract or standard of different concentrations solution.…”