Compound stress response in stomatal closure: a mathematical model of ABA and ethylene interaction in guard cells

Beguerisse-Díaz, Mariano; Hernandez-Gomez, Mercedes C.; Lizzul, Alessandro M; Barahona, Mauricio; Desikan, Radhika

doi:10.1186/1752-0509-6-146

Cited by 37 publications

(35 citation statements)

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“…ETH plays important roles in regulating plant development and metabolism, including leaf senescence, leaf abscission, and secondary metabolism [ 18 , 65 ]. In plants, ETH is synthesized from L-methionine by a number of enzymes, including 1-Aminocyclopropane-1-Carboxylate Synthase (ACS) and Aminocyclopropane Carboxylate Oxidase (ACO) [ 19 , 20 ].…”

Section: Resultsmentioning

confidence: 99%

Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress

et al. 2015

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BackgroundPhysic nut (Jatropha curcas L.) is a small perennial tree or large shrub, which is well-adapted to semi-arid regions and is considered to have potential as a crop for biofuel production. It is now regarded as an excellent model for studying biofuel plants. However, our knowledge about the molecular responses of this species to drought stress is currently limited.ResultsIn this study, genome-wide transcriptional profiles of roots and leaves of 8-week old physic nut seedlings were analyzed 1, 4 and 7 days after withholding irrigation. We observed a total of 1533 and 2900 differentially expressed genes (DEGs) in roots and leaves, respectively. Gene Ontology analysis showed that the biological processes enriched in droughted plants relative to unstressed plants were related to biosynthesis, transport, nucleobase-containing compounds, and cellular protein modification. The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose. Genes related to ABA signal transduction, and to trehalose and raffinose synthesis, were up-regulated in leaves. Endoplasmic reticulum (ER) stress response genes were significantly up-regulated in leaves under drought stress, while a number of genes related to wax biosynthesis were also up-regulated in leaves. Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation. As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.ConclusionsThis study provides us with new insights to increase our understanding of the response mechanisms deployed by physic nut seedlings under drought stress. The genes and pathways identified in this study also provide much information of potential value for germplasm improvement and breeding for drought resistance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0397-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress

et al. 2015

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“…Numerous studies have shown that H 2 O 2 and NO are involved in stomatal closure by multiple external stimuli or plant hormones, including ABA (Pei et al ., ; Desikan et al ., ; Neill et al ., ). The ethylene inhibition of ABA‐induced stomatal closure was also shown to be relative to an as‐yet‐unidentified antioxidant mechanism‐ and flavonol‐induced reduction in the level of ROS, including H 2 O 2 , in guard cells (Beguerisse‐Diaz et al ., ; Watkins et al ., ). Although H 2 O 2 and NO are known to mediate BR‐induced stress tolerance (Xia et al ., ; Cui et al ., ), the study of the role of H 2 O 2 in BR‐mediated stomatal movement is just beginning, and whether or not NO mediates the action of BR in stomatal behavior remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Although the role of ethylene in stomatal movement has been investigated for decades, its effect on this process seems rather inconsistent. In some investigations, ethylene was shown to open stomata (Levitt et al ., ) or to inhibit ABA‐induced stomatal closure (Tanaka et al ., ; Acharya and Assmann, ; Wilkinson and Davies, ; Beguerisse‐Diaz et al ., ; Chen et al ., ; Watkins et al ., ), or to reduce stomatal sensitivity to stresses (Benlloch‐Gonzalez et al ., ; Iqbal et al ., ), whereas in other works it was reported to close stomata (Pallas and Kays, ; Gunderson and Taylor, ; Young et al ., ; Desikan et al ., ). Yet the role of ethylene in BR‐regulated stomatal movement has not been studied.…”

Section: Introductionmentioning

confidence: 99%

Ethylene mediates brassinosteroid‐induced stomatal closure via Gα protein‐activated hydrogen peroxide and nitric oxide production in Arabidopsis

et al. 2015

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SUMMARYBrassinosteroids (BRs) are essential for plant growth and development; however, whether and how they promote stomatal closure is not fully clear. In this study, we report that 24-epibrassinolide (EBR), a bioactive BR, induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by triggering a signal transduction pathway including ethylene synthesis, the activation of Ga protein, and hydrogen peroxide (H 2 O 2 ) and nitric oxide (NO) production. EBR initiated a marked rise in ethylene, H 2 O 2 and NO levels, necessary for stomatal closure in the wild type. These effects were abolished in mutant bri1-301, and EBR failed to close the stomata of gpa1 mutants. Next, we found that both ethylene and Ga mediate the inductive effects of EBR on H 2 O 2 and NO production. EBR-triggered H 2 O 2 and NO accumulation were canceled in the etr1 and gpa1 mutants, but were strengthened in the eto1-1 mutant and the cGa line (constitutively overexpressing the G protein a-subunit AtGPA1). Exogenously applied H 2 O 2 or sodium nitroprusside (SNP) rescued the defects of etr1-3 and gpa1 or etr1 and gpa1 mutants in EBR-induced stomatal closure, whereas the stomata of eto1-1/AtrbohF and cGa/AtrbohF or eto1-1/nia1-2 and cGa/nia1-2 constructs had an analogous response to H 2 O 2 or SNP as those of AtrbohF or Nia1-2 mutants. Moreover, we provided evidence that Ga plays an important role in the responses of guard cells to ethylene. Ga activator CTX largely restored the lesion of the etr1-3 mutant, but ethylene precursor ACC failed to rescue the defects of gpa1 mutants in EBR-induced stomatal closure. Lastly, we demonstrated that Ga-activated H 2 O 2 production is required for NO synthesis. EBR failed to induce NO synthesis in mutant AtrbohF, but it led to H 2 O 2 production in mutant Nia1-2. Exogenously applied SNP rescued the defect of AtrbohF in EBR-induced stomatal closure, but H 2 O 2 did not reverse the lesion of EBR-induced stomatal closure in Nia1-2. Together, our results strongly suggest a signaling pathway in which EBR induces ethylene synthesis, thereby activating Ga, and then promotes AtrbohF-dependent H 2 O 2 production and subsequent Nia1-catalyzed NO accumulation, and finally closes stomata.

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“…Tansley review New Phytologist antioxidant mechanisms during stomatal closure: a slower, delayed response activated by a single stimulus (ABA or ET) and another more rapid mechanism that is only activated when both stimuli are present (Beguerisse-Diaz et al, 2012). Previous studies showed that an increased JA content of plants promoted stomatal closure in response to water stress and drought stress (Creelman & Mullet, 1995).…”

Section: Reviewmentioning

confidence: 99%

Behind the scenes: the roles of reactive oxygen species in guard cells

2013

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1121I.1121II.1122III.1125IV.1129V.11331134References1134 Summary Guard cells regulate stomatal pore size through integration of both endogenous and environmental signals; they are widely recognized as providing a key switching mechanism that maximizes both the efficient use of water and rates of CO2 exchange for photosynthesis; this is essential for the adaptation of plants to water stress. Reactive oxygen species (ROS) are widely considered to be an important player in guard cell signalling. In this review, we focus on recent progress concerning the role of ROS as signal molecules in controlling stomatal movement, the interaction between ROS and intrinsic and environmental response pathways, the specificity of ROS signalling, and how ROS signals are sensed and relayed. However, the picture of ROS‐mediated signalling is still fragmented and the issues of ROS sensing and the specificity of ROS signalling remain unclear. Here, we review some recent advances in our understanding of ROS signalling in guard cells, with an emphasis on the main players known to interact with abscisic acid signalling.

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Compound stress response in stomatal closure: a mathematical model of ABA and ethylene interaction in guard cells

Cited by 37 publications

References 90 publications

Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress

Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress

Ethylene mediates brassinosteroid‐induced stomatal closure via Gα protein‐activated hydrogen peroxide and nitric oxide production in Arabidopsis

Behind the scenes: the roles of reactive oxygen species in guard cells

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