Compound green tea (CGT) regulates lipid metabolism in high-fat diet induced mice

Zhou, Caibi; Hu, Liuhong; Mu, Ren; Mei, Xin; Wu, Xin; Wang, Chuanming; Zhou, Xiaolu

doi:10.1039/d2ra02831j

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…The extract was analyzed using a UPLC-ESI-MS/MS system (UPLC, SHI-MADZU Nexera X2, https://www.shimadzu.com.cn/, accessed on 9 November 2021; MS, Applied Biosystems 4500 Q TRAP, https:// www.thermofisher.cn/cn/zh/home/brands/applied-biosystems.html, accessed on 9 November 2021) with the analytical conditions described by Zhou et al (2022). The effluent was alternately connected to the ESI-triple quadrupole-linear ion trap (QTRAP)-MS.…”

Section: Uplc and Esi-q Trap-ms/ms Conditionsmentioning

confidence: 99%

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Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

Zhou,

Li,

et al. 2024

Physiologia Plantarum

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Mussaenda pubescens (Mp) is a valuable medicinal plant that has traditionally been used for medicinal purposes or as a tea substitute. However, there are few studies on the comprehensive and dynamic evaluation of Mp metabolites. This study used an ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) approach and biochemical analysis to investigate substance changes in leaves at three different stages and elucidate the relationship between metabolites and antioxidant capacity. The findings showed that Mp leaves contained 957 metabolites, the majority of which were phenolic acids, lipids, and terpenoids. The metabolite profiling of Mp leaves was significantly influenced by their growth and development at different stages. A total of 317 differentially accumulated metabolites (DAMs) were screened, including 150 primary metabolites and 167 secondary metabolites, with 202 DAMs found in bud leaf vs. tender leaf, 54 DAMs in tender leaf vs. mature leaf, and 254 DAMs in bud leaf vs. mature leaf. Total phenolics, flavonoids, and anthocyanin concentrations decreased as Mp leaves grew and developed, whereas terpenoids increased significantly. The secondary metabolites also demonstrated a positive correlation with antioxidant activity. Phenolics, flavonoids, terpenoids, and anthocyanins were the primary factors influencing the antioxidant activity of leaves. These findings provide new insights into the metabolite formation mechanism, as well as the development and utilization of Mp tea.

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Section: Uplc and Esi-q Trap-ms/ms Conditionsmentioning

confidence: 99%

“…The ion spray voltage (IS) was set to 5500 V and À 4500 V in positive and negative ion modes. Other ESI source operation parameters were the same as the Zhou et al (2022) protocol.…”

Section: Uplc and Esi-q Trap-ms/ms Conditionsmentioning

confidence: 99%

Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

Zhou,

Li,

et al. 2024

Physiologia Plantarum

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show abstract

“…Analysis of extract was performed using an UPLC-ESI-MS/MS system (UPLC, SHIMADZU Nexera X2, https://www.shimadzu.com.cn/, accessed on 9 November 2021; MS, Applied Biosystems 4500 Q TRAP, https://www.thermo sher.cn/cn/zh/home/brands/applied-biosystems.html, accessed on 9 November 2021) with the analytical conditions following the method described by Zhou et al [15]. The e uent was alternately connected to ESI-triple quadrupole-linear ion trap (QTRAP)-MS.…”

Section: Uplc and Esi-q Trap-ms/ms Conditionsmentioning

confidence: 99%

“…The ion spray voltage (IS) was set to 5500 V and − 4500 V in positive and negative ion modes. And other ESI source operation parameters were same as the protocol [15].…”

Section: Uplc and Esi-q Trap-ms/ms Conditionsmentioning

confidence: 99%

Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

Zhou,

Li,

et al. 2023

Preprint

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Background Mussaenda pubescens is an important source of medicinal plant that has been used as medicine or dried instead of tea. However, there are few studies on the thorough and dynamic assessment of metabolites presented in Mussaenda pubescens. Methods In this study, UHPLC-MS/MS approach and biochemical analysis were used to investigate the substance changes of leaves at different developmental stages. Result A total of 957 metabolites were identified, among which 202, 54 and 254 metabolites showed differential accumulation in three comparisons. The up-regulated metabolites were the major factors driving the function and quality of Mussaenda pubescens. The main pathways involved “Flavone and flavonol biosynthesis”, “Phenylalanine metabolism”, “ABC transporters”, “Folate biosynthesis”, and “Fructose and mannose metabolism”. Phenolics, flavonoids, terpenoids and anthocyanin were the primary contributors to differential antioxidant activities of bud, tender, and mature leaves. Conclusions These results provide a novel insight into formation mechanism of metabolites and the development of Mussaenda pubescenstea.

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Untargeted metabolomics reveals the regulatory effect of geniposidic acid on lipid accumulation in HepG2 cells and Caenorhabditis elegans and validation in hyperlipidemic hamsters

Huang,

Gu,

Nie

et al. 2024

Phytomedicine

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Compound green tea (CGT) regulates lipid metabolism in high-fat diet induced mice

Abstract: Compound green tea is able to decrease the body weight and regulate the plasma lipid indexes, as well as make the abnormal metabolic state return to normal by intervening in different metabolic pathways to partially reverse the lipid metabolism disorder.

Cited by 4 publications

References 42 publications

Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

Untargeted metabolomics reveals the regulatory effect of geniposidic acid on lipid accumulation in HepG2 cells and Caenorhabditis elegans and validation in hyperlipidemic hamsters

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